The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Structural basis of aquaporin-4 autoantibody binding in neuromyelitis optica
Open accessPublished version of a preprint
Abstract
Neuromyelitis optica (NMO) is an autoimmune disease of the central nervous system where pathogenic autoantibodies target the water channel aquaporin-4 on human astrocytes causing neurological impairment. Autoantibody binding leads to complement-dependent and complement-independent cytotoxicity, ultimately resulting in astrocyte death, demyelination, and neuronal loss. Aquaporin-4 assembles in astrocyte plasma membranes as symmetric tetramers or as arrays of tetramers. We report molecular structures of aquaporin-4 alone and bound to Fab fragments from patient-derived NMO autoantibodies using cryogenic electron microscopy. Each antibody binds to epitopes comprised of three extracellular loops of aquaporin-4 with contributions from multiple molecules in the assembly. The structures distinguish between antibodies that bind to the tetrameric form of aquaporin-4 and those targeting higher-order orthogonal arrays of tetramers that provide more diverse bridging epitopes.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Highly accurate protein structure prediction with AlphaFold
Jumper, J., Evans, R., Pritzel, A., Green, T., Figurnov, M., Ronneberger, O., Tunyasuvunakool, K., Bates, R., Žídek, A., Potapenko, A., et al. (2021) · Nature · doi:10.1038/s41586-021-03819-2 · Cited by 43,764
Flagged first, then by citations
Research works printed but not linked: 34
- 10.1038/s41572-020-0214-9 · DOI
- 10.1097/WNO.0000000000000508 · DOI
- 10.1007/s00401-013-1220-8 · DOI
- 10.1002/ana.24630 · DOI
- 10.1002/ana.21802 · DOI
- 10.1007/s00401-013-1172-z · DOI
- 10.1093/brain/awac079 · DOI
- 10.1172/JCI122942 · DOI
- 10.1016/S1474-4422(20)30392-6 · DOI
- 10.1242/jcs.042341 · DOI
- 10.1074/jbc.M110.123000 · DOI
- 10.1074/jbc.M115.647149 · DOI
- 10.1016/j.jneuroim.2011.05.001 · DOI
- 10.1073/pnas.0902725106 · DOI
- 10.1002/glia.20855 · DOI
- 10.1074/jbc.M111.227298 · DOI
- 10.1016/S0006-3495(93)81293-1 · DOI
- 10.1016/j.jmb.2005.10.081 · DOI
- 10.1073/pnas.1012867108 · DOI
- 10.1523/JNEUROSCI.1987-15.2015 · DOI
- 10.1002/glia.23231 · DOI
- 10.1002/jnr.22822 · DOI
- 10.1002/glia.22417 · DOI
- 10.1073/pnas.1109980108 · DOI
- 10.1016/j.jmb.2007.05.022 · DOI
- P. L. Chiu J. D. Orjuela B. L. de Groot C. Aponte-Santamaria T. Walz Structure and dynamics of cholesterol-mediated aquaporin-0 arrays and implications for lipid rafts. bioRxiv 2023.05.16.540959 (2023); https://www.biorxiv.org/content/10.1101/2023.05.16.540959v3. · DOI
- 10.1038/nature04321 · DOI
- 10.1073/pnas.0405274101 · DOI
- 10.3389/fcell.2022.866980 · DOI
- 10.1002/glia.22635 · DOI
- 10.1016/j.bpj.2017.03.012 · DOI
- 10.1096/fj.11-201608 · DOI
- 10.1038/nmeth.4169 · DOI · Paper page
- 10.7554/eLife.01456 · DOI
Metadata
- Authors
- Gupta, M., Khandelwal, N. K., Nelson, A., Hwang, P., Pourmal, S., Bennett, J. L., & Stroud, R. M.
- Affiliations
- Oregon Health & Science University (US)
- University of California, San Francisco (US)
- University of Colorado Anschutz (US)
- Corresponding author
- Gupta, Meghna
- Bennett, Jeffrey L.
- Stroud, Robert M.
- Venue
- Science Advances
- Published
- 2025-02-21
- Volume
- 11
- Issue
- 8
- Pages
- eadq7560-eadq7560
- ISSN
- 2375-2548 (ISSN-L)
- PMC
- PMC11844742
- OpenAlex
- W4407802849
- Cited by
- 11
- References
- 1
- Citations by year
- 2026: 6 · 2025: 4
- FWCI
- 2.99
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- cc-by
- Locations
- Science Advances (publishedVersion, cc-by)
- bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by-nd)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- eScholarship (California Digital Library) (submittedVersion, cc-by)
- PubMed Central (submittedVersion, cc-by-nd)
- Funders
- National Institutes of Health (1s10od026881-01, 1s10od021741-01, 1s10od020054-01)
- National Institute on Aging (K99AG070271)
- National Eye Institute (R01EY022936)
- National Institute of General Medical Sciences (R01GM024485, R35GM156263, T32GM145460)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Ion Transport and Channel Regulation
- Health Sciences › Medicine › Clinical Neurology › Multiple Sclerosis Research Studies
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Structural Biology › Enzyme Structure and Function
- Keywords
- neuromyelitis optica, aquaporin-4, autoantibodies, antibody-antigen binding, astrocytes, aquaporins, tetramer assembly, extracellular loop, antigenic determinants, cryo-EM structure, demyelination, complement-dependent cytotoxicity
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
