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Functional characterization of dynamic nascent RNA folding ensembles in real time
Open accessPublished version of a preprint
Abstract
RNA structure starts forming cotranscriptionally as the nascent RNA emerges from the RNA polymerase and is dynamically modulated by cellular factors. How individual RNA conformations, out of an ensemble of RNA molecules, relate to function is not well understood. Here, developing multicolor single-molecule fluorescence microscopy experiments, we track in real time nascent RNA structure formation, functionally characterizing up to eight different types of RNA molecules. We find that ribosomal proteins, RNA modification enzymes or antisense oligonucleotides specifically modulate a subset of the RNA folding classes. For example, we provide direct evidence that increased local RNA accessibility at specific sites correlates with the chaperoning activity of ribosomal proteins during ribosome assembly. These experiments provide a general framework to study how dynamic RNA folding, and misfolding, relates to function.
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Highly accurate protein structure prediction with AlphaFold
Jumper, J., Evans, R., Pritzel, A., Green, T., Figurnov, M., Ronneberger, O., Tunyasuvunakool, K., Bates, R., Žídek, A., Potapenko, A., et al. (2021) · Nature · doi:10.1038/s41586-021-03819-2 · Cited by 43,764
Accurate structure prediction of biomolecular interactions with AlphaFold 3
Abramson, J., Adler, J., Dunger, J., Evans, R., Green, T., Pritzel, A., Ronneberger, O., Willmore, L., Ballard, A. J., Bambrick, J., et al. (2024) · Nature · doi:10.1038/s41586-024-07487-w · Cited by 15,861
Research works printed but not linked: 96
- 10.1261/rna.5205404 · DOI
- 10.1073/pnas.96.16.9077 · DOI
- 10.1126/sciadv.abc9191 · DOI
- 10.1093/nar/gkad1120 · DOI
- 10.1038/s41467-023-36184-x · DOI · Paper page
- 10.1038/nature06389 · DOI
- 10.1126/science.abe5650 · DOI
- 10.1038/s41592-024-02487-0 · DOI
- 10.1038/s41592-021-01075-w · DOI
- 10.1038/s41586-020-2253-5 · DOI
- 10.1016/j.molcel.2022.02.009 · DOI
- 10.1038/s41586-022-05135-9 · DOI
- 10.1126/science.8052848 · DOI
- 10.1073/pnas.0705038104 · DOI
- 10.1021/acschembio.3c00435 · DOI
- 10.1038/nchembio.562 · DOI
- 10.1038/ncomms9976 · DOI
- 10.1073/pnas.1304585110 · DOI
- 10.1038/s41467-018-04305-6 · DOI
- 10.1021/bi048533y · DOI
- 10.1073/pnas.2109026118 · DOI
- 10.1038/s41467-018-07423-3 · DOI
- 10.1016/j.cell.2019.10.035 · DOI
- 10.1126/science.1225722 · DOI
- 10.1038/s41467-020-18283-1 · DOI
- 10.1016/j.cell.2019.11.007 · DOI
- 10.1073/pnas.1712983115 · DOI
- 10.1146/annurev-biochem-062608-160432 · DOI
- 10.1146/annurev.biophys.050708.133615 · DOI
- 10.3390/biom13050866 · DOI
- 10.1016/j.cbpa.2008.09.024 · DOI
- 10.1016/0022-2836(88)90243-4 · DOI
- T. Powers, H. F. Noller, Hydroxyl radical footprinting of ribosomal proteins on 16S rRNA. RNA 1, 194–209 (1995).
- 10.1016/0022-2836(88)90468-8 · DOI
- 10.1126/science.2658053 · DOI
- 10.1016/S0021-9258(19)42644-6 · DOI
- 10.1038/2261214a0 · DOI
- 10.1038/nature07298 · DOI
- 10.1016/j.jmb.2005.06.020 · DOI
- 10.1038/nature04261 · DOI
- 10.1038/nature13039 · DOI
- 10.1126/science.1193220 · DOI
- 10.3390/biom13060951 · DOI
- 10.1038/nsmb.2294 · DOI
- 10.1021/acssynbio.9b00471 · DOI
- 10.1016/j.bpj.2013.05.061 · DOI
- 10.1006/jmbi.1993.1396 · DOI
- 10.1038/nbt.3246 · DOI
- 10.1016/j.tibs.2021.05.009 · DOI
- 10.1074/jbc.270.36.20871 · DOI
- 10.1016/j.jmb.2022.167665 · DOI
- 10.1093/nar/19.25.7089 · DOI
- 10.1021/ja412084b · DOI
- 10.1093/nar/gks508 · DOI · Paper page
- 10.1038/nature12894 · DOI
- 10.1016/j.cell.2016.11.020 · DOI
- 10.1021/cb3001606 · DOI
- 10.1038/s41589-019-0384-5 · DOI
- 10.1038/s41573-022-00521-4 · DOI
- 10.1128/AAC.46.5.1546-1549.2002 · DOI
- 10.1038/nchembio.596 · DOI
- 10.1038/s41467-024-49638-7 · DOI
- 10.1038/s41586-024-07559-x · DOI · Paper page
- 10.1038/s41586-024-08336-6 · DOI
- 10.1016/j.chembiol.2023.09.005 · DOI
- 10.1093/nar/29.17.3664 · DOI
- 10.1016/j.omtn.2024.102186 · DOI
- 10.1089/nat.2022.0060 · DOI
- 10.1093/nar/gkl715 · DOI
- 10.1016/S0021-9258(18)55250-9 · DOI
- 10.1038/nm0696-668 · DOI
- A. S. Alharbi, A. J. Garcin, K. A. Lennox, S. Pradeloux, C. Wong, S. Straub, R. Valentin, G. Pepin, H. M. Li, M. F. Nold, C. A. Nold-Petry, M. A. Behlke, M. P. Gantier, Rational design of antisense oligonucleotides modulating the activity of TLR7/8 agonists. Nucleic Acids Res. 48, 7052–7065 (2020).
- 10.1073/pnas.0912007107 · DOI
- 10.1016/j.jmb.2012.11.040 · DOI
- 10.1016/j.molcel.2013.09.020 · DOI
- 10.1126/sciadv.abq4144 · DOI
- 10.1073/pnas.2209146119 · DOI
- 10.1038/s41467-024-52914-1 · DOI
- 10.1016/S0959-440X(99)80045-1 · DOI
- 10.1016/S0959-440X(00)00206-2 · DOI
- 10.1126/science.271.5247.345 · DOI
- 10.1038/s41589-019-0382-7 · DOI
- 10.1038/nsmb.3316 · DOI
- 10.1126/science.279.5358.1943 · DOI
- 10.1093/dnares/dsi012 · DOI
- 10.1038/s41586-024-08308-w · DOI
- S. D. Chandradoss, A. C. Haagsma, Y. K. Lee, J. H. Hwang, J. M. Nam, C. Joo, Surface passivation for single-molecule protein studies. J. Vis. Exp., 50549 (2014).
- 10.1529/biophysj.107.117689 · DOI
- 10.1038/nmeth934 · DOI
- 10.1038/nmeth.3769 · DOI
- 10.1073/pnas.1315735111 · DOI
- 10.1038/nsmb.2567 · DOI
- 10.1038/s41586-022-04858-z · DOI · Paper page
- 10.1016/0003-9861(86)90568-0 · DOI
- 10.1093/nar/gkq808 · DOI
- 10.1002/jcp.1040740428 · DOI
Metadata
- Authors
- Gor, K., Geissen, E. M., & Duss, O.
- Affiliations
- European Molecular Biology Laboratory (DE)
- Corresponding author
- Duss, Olivier
- Venue
- Science Advances
- Published
- 2026-03-20
- Volume
- 12
- Issue
- 12
- Pages
- eaec4037-eaec4037
- ISSN
- 2375-2548 (ISSN-L)
- OpenAlex
- W7139960558
- Cited by
- 4
- References
- 2
- Citations by year
- 2026: 4
- FWCI
- 8.21
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- cc-by-nc
- Locations
- Science Advances (publishedVersion, cc-by-nc)
- bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by-nc)
- PubMed (publishedVersion)
- Funders
- Deutsche Forschungsgemeinschaft (512397425)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Structure and Dynamics
- Keywords
- ribosomal proteins, ribosome assembly, RNA accessibility, RNA-modifying enzymes, antisense oligonucleotides, RNA misfolding, single-molecule fluorescence microscopy, multicolor imaging
- MeSH terms
- Microscopy, Fluorescence, Nucleic Acid Conformation, Ribosomal Proteins, Ribosomes, RNA, RNA Folding
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