The DOI matches its Crossref record.
Biosynthesis of Complex Polyketides in a Metabolically Engineered Strain of E. coli
Abstract
The macrocyclic core of the antibiotic erythromycin, 6-deoxyerythronolide B (6dEB), is a complex natural product synthesized by the soil bacterium Saccharopolyspora erythraea through the action of a multifunctional polyketide synthase (PKS). The engineering potential of modular PKSs is hampered by the limited capabilities for molecular biological manipulation of organisms (principally actinomycetes) in which complex polyketides have thus far been produced. To address this problem, a derivative of Escherichia coli has been genetically engineered. The resulting cellular catalyst converts exogenous propionate into 6dEB with a specific productivity that compares well with a high-producing mutant of S. erythraea that has been incrementally enhanced over decades for the industrial production of erythromycin.
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Cited by
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Wargacki, A. J., Leonard, E., Win, M. N., Regitsky, D. D., Santos, C. N. S., Kim, P. B., Cooper, S. R., Raisner, R. M., Herman, A., Sivitz, A. B., et al. (2012) · Science · doi:10.1126/science.1214547 · Cited by 734
Combinatorial biosynthesis of reduced polyketides
Weissman, K. J., & Leadlay, P. F. (2005) · Nature Reviews Microbiology · doi:10.1038/nrmicro1287 · Cited by 466
Biosynthesis of polyketide synthase extender units
Chan, Y. A., Podevels, A. M., Kevany, B. M., & Thomas, M. G. (2009) · Nat. Prod. Rep. · doi:10.1039/b801658p · Cited by 337
Newman, J. D., Marshall, J., Chang, M., Nowroozi, F., Paradise, E., Pitera, D., Newman, K. L., & Keasling, J. D. (2006) · Biotechnology and Bioengineering · doi:10.1002/bit.21017 · Cited by 257
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Metadata
- Authors
- Pfeifer, B. A., Admiraal, S. J., Gramajo, H., Cane, D. E., & Khosla, C.
- Venue
- Science
- Published
- 2001-03-02
- Volume
- 291
- Issue
- 5509
- Pages
- 1790-1792
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2076915648
- Cited by
- 750
- References
- 0
- FWCI
- 14.57
- Type
- journal article
- Language
- en
- Open access
- closed
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- Pfeifer, B. A., Admiraal, S. J., Gramajo, H., Cane, D. E., & Khosla, C. (2001). Biosynthesis of Complex Polyketides in a Metabolically Engineered Strain of E. coli. Science. https://doi.org/10.1126/science.1058092
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
