High‐level production of amorpha‐4,11‐diene in a two‐phase partitioning bioreactor of metabolically engineered Escherichia coli
Abstract
Reconstructing synthetic metabolic pathways in microbes holds great promise for the production of pharmaceuticals in large-scale fermentations. By recreating biosynthetic pathways in bacteria, complex molecules traditionally harvested from scarce natural resources can be produced in microbial cultures. Here we report on a strain of Escherichia coli containing a heterologous, nine-gene biosynthetic pathway for the production of the terpene amorpha-4,11-diene, a precursor to the anti-malarial drug artemisinin. Previous reports have underestimated the productivity of this strain due to the volatility of amorphadiene. Here we show that amorphadiene evaporates from a fermentor with a half-life of about 50 min. Using a condenser, we take advantage of this volatility by trapping the amorphadiene in the off-gas. Amorphadiene was positively identified using nuclear magnetic resonance spectroscopy and determined to be 89% pure as collected. We captured amorphadiene as it was produced in situ by employing a two-phase partitioning bioreactor with a dodecane organic phase. Using a previously characterized caryophyllene standard to calibrate amorphadiene production and capture, the concentration of amorphadiene produced was determined to be 0.5 g/L of culture medium. A standard of amorphadiene collected from the off-gas showed that the caryophyllene standard overestimated amorphadiene production by approximately 30%.
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Both Saccharomyces cerevisiae and Escherichia coli have been used as hosts when attempting to establish a viable recombinant microbial pathway for artemisinin biosynthesis.
“Both Saccharomyces cerevisiae [379,380] and Escherichia coli [381,382,383] have been used as hosts when attempting to establish a viable recombinant microbial pathway for the biosynthesis of artemisinin.”
From The Biosynthesis of Artemisinin (Qinghaosu) and the Phytochemistry of Artemisia annua L. (Qinghao) (2010) · cites this paper as [380]
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- Authors
- Newman, J. D., Marshall, J., Chang, M., Nowroozi, F., Paradise, E., Pitera, D., Newman, K. L., & Keasling, J. D.
- Affiliations
- University of California, Berkeley (US)
- Lawrence Berkeley National Laboratory (US)
- Corresponding author
- Keasling, Jay D.
- Venue
- Biotechnology and Bioengineering
- Publisher
- Wiley
- Published
- 2006-07-28
- Volume
- 95
- Issue
- 4
- Pages
- 684-691
- ISSN
- 0006-3592 (ISSN-L)
- 1097-0290
- OpenAlex
- W2070190792
- Cited by
- 257
- References
- 16
- Citations by year
- 2026: 3 · 2025: 5 · 2024: 6 · 2023: 3 · 2022: 8 · 2021: 15 · 2020: 11 · 2019: 19 · 2018: 17 · 2017: 6 · 2016: 13 · 2015: 14 · 2014: 22 · 2013: 18 · 2012: 26
- FWCI
- 5.84
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Biotechnology and Bioengineering (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health (1r41ai061936-01)
- National Institute of Allergy and Infectious Diseases (5 R41 AI061936-02)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant biochemistry and biosynthesis
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Biochemical and biochemical processes
- Keywords
- terpene biosynthesis, Escherichia coli, metabolic engineering, two-phase partitioning bioreactor, n-dodecane
- MeSH terms
- Polycyclic Sesquiterpenes, Antimalarials, Escherichia coli, Fermentation, Gases, Half-Life, Sesquiterpenes, Terpenes, Volatilization, Bioreactors, Artemisinins
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