Cited by 1,247Genomics and Chromatin DynamicsEnzyme Structure and FunctionProtein Structure and Dynamics

The DOI matches its Crossref record; 71 of the 71 references it cites were found in a registry or an index.

Structural Basis of Transcription: RNA Polymerase II at 2.8 Ångstrom Resolution

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Cramer, Patrick, Bushnell, David A., Kornberg, Roger D.·2001·Science

Abstract

Structures of a 10-subunit yeast RNA polymerase II have been derived from two crystal forms at 2.8 and 3.1 angstrom resolution. Comparison of the structures reveals a division of the polymerase into four mobile modules, including a clamp, shown previously to swing over the active center. In the 2.8 angstrom structure, the clamp is in an open state, allowing entry of straight promoter DNA for the initiation of transcription. Three loops extending from the clamp may play roles in RNA unwinding and DNA rewinding during transcription. A 2.8 angstrom difference Fourier map reveals two metal ions at the active site, one persistently bound and the other possibly exchangeable during RNA synthesis. The results also provide evidence for RNA exit in the vicinity of the carboxyl-terminal repeat domain, coupling synthesis to RNA processing by enzymes bound to this domain.

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Crossref · 10.1126/science.1059493

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  1. 10.1016/0968-0004(96)10050-5 · DOI
  2. ; published online 19 April 2001 (10.1126/science.1059495). · DOI
  3. Werner M., Hermann-Le Denmat S., Treich I., Sentenac A., Thuriaux P., Mol. Cell. Biol. 12, 1087 (1992).
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  9. Cowtan K., Joint CCP4 ESF-EACBM Newslett. Protein Crystallogr. 31, 34 (1994).

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Metadata

Authors
Cramer, P., Bushnell, D. A., & Kornberg, R. D.
Affiliations
Stanford Medicine (US)
Corresponding author
  • Cramer, Patrick
  • Kornberg, Roger D.
Venue
Science
Published
2001-06-08
Volume
292
Issue
5523
Pages
1863-1876
ISSN
  • 0036-8075 (ISSN-L)
  • 1095-9203
OpenAlex
W2074206760
Cited by
1,247
References
71
Citations by year
2026: 18 · 2025: 16 · 2024: 40 · 2023: 29 · 2022: 29 · 2021: 38 · 2020: 38 · 2019: 48 · 2018: 34 · 2017: 51 · 2016: 33 · 2015: 37 · 2014: 50 · 2013: 55 · 2012: 63
FWCI
27.00
Type
journal article
Language
en
Open access
Licence
other-oa
Locations
  • Science (publishedVersion)
  • PubMed (publishedVersion)
  • Max Planck Digital Library (submittedVersion, other-oa)
  • GRO (submittedVersion)
  • MPG.PuRe (Max Planck Society) (submittedVersion, other-oa)
Funders
National Institute of General Medical Sciences (GM49985, R01GM049985)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Structural Biology › Enzyme Structure and Function
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Structure and Dynamics
Keywords
RNA polymerase II, transcription initiation, active site metal ions, RNA unwinding, promoter DNA, crystal structure
MeSH terms
Amino Acid Sequence, Binding Sites, DNA, Fungal, Fourier Analysis, Hydrogen Bonding, Magnesium, Metals, Models, Molecular, Molecular Sequence Data, Promoter Regions, Genetic, Protein Conformation, RNA Polymerase II, RNA Processing, Post-Transcriptional, RNA, Fungal, RNA, Messenger, Saccharomyces cerevisiae, Transcription Factors, Transcription, Genetic, Conserved Sequence, Protein Structure, Secondary, Protein Structure, Tertiary, Crystallography, X-Ray, Protein Structure, Quaternary, Protein Subunits

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