The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Partitioning of Lipid-Modified Monomeric GFPs into Membrane Microdomains of Live Cells
Abstract
Many proteins associated with the plasma membrane are known to partition into submicroscopic sphingolipid- and cholesterol-rich domains called lipid rafts, but the determinants dictating this segregation of proteins in the membrane are poorly understood. We suppressed the tendency of Aequorea fluorescent proteins to dimerize and targeted these variants to the plasma membrane using several different types of lipid anchors. Fluorescence resonance energy transfer measurements in living cells revealed that acyl but not prenyl modifications promote clustering in lipid rafts. Thus the nature of the lipid anchor on a protein is sufficient to determine submicroscopic localization within the plasma membrane.
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Cites
Pike, L. J., & Miller, J. M. (1998) · Journal of Biological Chemistry · doi:10.1074/jbc.273.35.22298 · Cited by 374
Cited by
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Campbell, R. E., Tour, O., Palmer, A. E., Steinbach, P. A., Baird, G. S., Zacharias, D. A., & Tsien, R. Y. (2002) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.082243699 · Cited by 2,365
Self-citationTransgenic strategies for combinatorial expression of fluorescent proteins in the nervous system
Livet, J., Weissman, T. A., Kang, H., Draft, R. W., Lu, J., Bennis, R. A., Sanes, J. R., & Lichtman, J. W. (2007) · Nature · doi:10.1038/nature06293 · Cited by 1,876
Principles of two-photon excitation microscopy and its applications to neuroscience
Svoboda, K., & Yasuda, R. (2006) · Neuron · doi:10.1016/j.neuron.2006.05.019 · Cited by 1,027
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Lippincott-Schwartz, J., & Patterson, G. H. (2003) · Science · doi:10.1126/science.1082520 · Cited by 992
Cholesterol, lipid rafts, and disease
Simons, K., & Ehehalt, R. (2002) · Journal of Clinical Investigation · doi:10.1172/jci0216390 · Cited by 916
Metadata
- Authors
- Zacharias, D. A., Violin, J. D., Newton, A. C., & Tsien, R. Y.
- Venue
- Science
- Published
- 2002-05-03
- Volume
- 296
- Issue
- 5569
- Pages
- 913-916
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2055864348
- Cited by
- 2,235
- References
- 1
- FWCI
- 33.91
- Type
- journal article
- Language
- en
- Open access
- closed
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
