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A Kinetic Framework for a Mammalian RNA Polymerase in Vivo
Abstract
We have analyzed the kinetics of assembly and elongation of the mammalian RNA polymerase I complex on endogenous ribosomal genes in the nuclei of living cells with the use of in vivo microscopy. We show that components of the RNA polymerase I machinery are brought to ribosomal genes as distinct subunits and that assembly occurs via metastable intermediates. With the use of computational modeling of imaging data, we have determined the in vivo elongation time of the polymerase, and measurements of recruitment and incorporation frequencies show that incorporation of components into the assembling polymerase is inefficient. Our data provide a kinetic and mechanistic framework for the function of a mammalian RNA polymerase in living cells.
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Citations
Cited by
Prescher, J. A., & Bertozzi, C. R. (2005) · Nature Chemical Biology · doi:10.1038/nchembio0605-13 · Cited by 1,456
In vivo dynamics of RNA polymerase II transcription
Darzacq, X., Shav-Tal, Y., de Turris, V., Brody, Y., Shenoy, S. M., Phair, R. D., & Singer, R. H. (2007) · Nature Structural & Molecular Biology · doi:10.1038/nsmb1280 · Cited by 665
Self-citationAnalysis of Binding Reactions by Fluorescence Recovery after Photobleaching
Sprague, B. L., Pego, R. L., Stavreva, D. A., & McNally, J. G. (2004) · Biophysical Journal · doi:10.1529/biophysj.103.026765 · Cited by 616
Life on a planet of its own: Regulation of RNA polymerase I transcription in the nucleolus
Grummt, I. (2003) · Genes & Development · doi:10.1101/gad.1098503r · Cited by 540
Self-citationEukaryotic transcriptional dynamics: from single molecules to cell populations
Coulon, A., Chow, C. C., Singer, R. H., & Larson, D. R. (2013) · Nature Reviews Genetics · doi:10.1038/nrg3484 · Cited by 309
Metadata
- Authors
- Dundr, M., Hoffmann-Rohrer, U., Hu, Q., Grummt, I., Rothblum, L. I., Phair, R. D., & Misteli, T.
- Venue
- Science
- Published
- 2002-11-22
- Volume
- 298
- Issue
- 5598
- Pages
- 1623-1626
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2037228253
- Cited by
- 436
- References
- 0
- FWCI
- 11.77
- Type
- journal article
- Language
- en
- Open access
- closed
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- Dundr, M., Hoffmann-Rohrer, U., Hu, Q., Grummt, I., Rothblum, L. I., Phair, R. D., & Misteli, T. (2002). A Kinetic Framework for a Mammalian RNA Polymerase in Vivo. Science. https://doi.org/10.1126/science.1076164
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
