In vivo dynamics of RNA polymerase II transcription
Open accessAbstract
We imaged transcription in living cells using a locus-specific reporter system, which allowed precise, single-cell kinetic measurements of promoter binding, initiation and elongation. Photobleaching of fluorescent RNA polymerase II revealed several kinetically distinct populations of the enzyme interacting with a specific gene. Photobleaching and photoactivation of fluorescent MS2 proteins used to label nascent messenger RNAs provided sensitive elongation measurements. A mechanistic kinetic model that fits our data was validated using specific inhibitors. Polymerases elongated at 4.3 kilobases min(-1), much faster than previously documented, and entered a paused state for unexpectedly long times. Transcription onset was inefficient, with only 1% of polymerase-gene interactions leading to completion of an mRNA. Our systems approach, quantifying both polymerase and mRNA kinetics on a defined DNA template in vivo with high temporal resolution, opens new avenues for studying regulation of transcriptional processes in vivo.
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RNA Pol II speed in live cells was estimated to range from 0.6 kb/min to 4.3 kb/min by FRAP experiments using MS2 fluorescent proteins.
“RNA Pol II speed in live cells was estimated to range from 0.6 kb/min to 4.3 kb/min by fluorescence recovery after photobleaching (FRAP) experiments using MS2 fluorescent proteins to label nascent MS2 repeat‐containing RNAs (Boireau et al, 2007; Darzacq et al, 2007; Ben‐Ari et al, 2010; Yunger et al, 2010; Brody et al, 2011; Maiuri et al, 2011; Muramoto et al, 2012; Corrigan et al, 2016).”
From RNA polymerase II speed: a key player in controlling and adapting transcriptome composition (2021) · cites this paper as Darzacq et al, 2007
Cites
A new look at the statistical model identification
Akaike, H. (1974) · IEEE Transactions on Automatic Control · doi:10.1109/tac.1974.1100705 · Cited by 51,879 · see citing works
Estimating the Dimension of a Model
Schwarz, G. (1978) · The Annals of Statistics · doi:10.1214/aos/1176344136 · Cited by 47,033 · see citing works
Localization of ASH1 mRNA particles in living yeast.
Bertrand, E., Chartrand, P., Schaefer, M., Shenoy, S., Singer, R., & Long, R. M. (1998) · Molecules and Cells · doi:10.1016/s1097-2765(00)80143-4 · Cited by 1,597 · see citing works
Self-citationVisualization of Single RNA Transcripts in Situ
Femino, A. M., Fay, F. S., Fogarty, K., & Singer, R. H. (1998) · Science · doi:10.1126/science.280.5363.585 · Cited by 1,504 · see citing works
Self-citationStructural Basis of Transcription: RNA Polymerase II at 2.8 Ångstrom Resolution
Cramer, P., Bushnell, D. A., & Kornberg, R. D. (2001) · Science · doi:10.1126/science.1059493 · Cited by 1,247 · see citing works
High mobility of proteins in the mammalian cell nucleus
Phair, R. D., & Misteli, T. (2000) · Nature · doi:10.1038/35007077 · Cited by 1,212 · see citing works
Self-citationControlling the elongation phase of transcription with P-TEFb
Peterlin, B. M., & Price, D. H. (2006) · Molecular Cell · doi:10.1016/j.molcel.2006.06.014 · Cited by 1,144 · see citing works
From Birth to Death: The Complex Lives of Eukaryotic mRNAs
Moore, M. J. (2005) · Science · doi:10.1126/science.1111443 · Cited by 1,003 · see citing works
Anti-termination of transcription within the long terminal repeat of HIV-1 by tat gene product
Kao, S.-Y., Calman, A. F., Luciw, P. A., & Peterlin, B. M. (1987) · Nature · doi:10.1038/330489a0 · Cited by 908 · see citing works
Robinett, C. C., Straight, A. F., Li, G., Willhelm, C., Sudlow, G., Murray, A. W., & Belmont, A. S. (1996) · The Journal of Cell Biology · doi:10.1083/jcb.135.6.1685 · Cited by 720 · see citing works
Transcriptional Pulsing of a Developmental Gene
Chubb, J. R., Trcek, T., Shenoy, S. M., & Singer, R. H. (2006) · Current Biology · doi:10.1016/j.cub.2006.03.092 · Cited by 714 · see citing works
Self-citationFrom Silencing to Gene Expression
Janicki, S. M., Tsukamoto, T., Salghetti, S. E., Tansey, W. P., Sachidanandam, R., Prasanth, K. V., Ried, T., Shav-Tal, Y., Bertrand, E., Singer, R. H., et al. (2004) · Cell · doi:10.1016/s0092-8674(04)00171-0 · Cited by 704 · see citing works
Self-citationRougvie, A. E., & Lis, J. T. (1988) · Cell · doi:10.1016/s0092-8674(88)91087-2 · Cited by 680 · see citing works
Transcriptional noise and the fidelity of initiation by RNA polymerase II
Struhl, K. (2007) · Nature Structural & Molecular Biology · doi:10.1038/nsmb0207-103 · Cited by 643 · see citing works
Single mRNA Molecules Demonstrate Probabilistic Movement in Living Mammalian Cells
Fusco, D. N., Accornero, N., Lavoie, B. A., Shenoy, S. M., Blanchard, J., Singer, R. H., & Bertrand, É. (2003) · Current Biology · doi:10.1016/s0960-9822(02)01436-7 · Cited by 583 · see citing works
Self-citationMechanism of Action of Camptothecin
Liu, L. F., Duann, P., Lin, C., D’Arpa, P., & Wu, J. (1996) · Annals of the New York Academy of Sciences · doi:10.1111/j.1749-6632.1996.tb26375.x · Cited by 517 · see citing works
Breaking barriers to transcription elongation
Saunders, A., Core, L. J., & Lis, J. T. (2006) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm1981 · Cited by 506 · see citing works
Dynamics of Single mRNPs in Nuclei of Living Cells
Shav‐Tal, Y., Darzacq, X., Shenoy, S. M., Fusco, D. N., Janicki, S. M., Spector, D. L., & Singer, R. H. (2004) · Science · doi:10.1126/science.1099754 · Cited by 494 · see citing works
Self-citationRules of engagement: co-transcriptional recruitment of pre-mRNA processing factors
Bentley, D. L. (2005) · Current Opinion in Cell Biology · doi:10.1016/j.ceb.2005.04.006 · Cited by 489 · see citing works
Mechanism of Action of Camptothecin
Liu, L. F., Desai, S. D., Li, T., Mao, Y., Sun, M., & Sim, S.-P. (2000) · Annals of the New York Academy of Sciences · doi:10.1111/j.1749-6632.2000.tb07020.x · Cited by 476 · see citing works
Initial Transcription by RNA Polymerase Proceeds Through a DNA-Scrunching Mechanism
Kapanidis, A. N., Margeat, E., Ho, S. O., Kortkhonjia, E., Weiss, S., & Ebright, R. H. (2006) · Science · doi:10.1126/science.1131399 · Cited by 453 · see citing works
A Kinetic Framework for a Mammalian RNA Polymerase in Vivo
Dundr, M., Hoffmann‐Rohrer, U., Hu, Q., Grummt, I., Rothblum, L. I., Phair, R. D., & Misteli, T. (2002) · Science · doi:10.1126/science.1076164 · Cited by 436 · see citing works
Self-citationThe human dystrophin gene requires 16 hours to be transcribed and is cotranscriptionally spliced
Tennyson, C. N., Klamut, H. J., & Worton, R. G. (1995) · Nature Genetics · doi:10.1038/ng0295-184 · Cited by 369 · see citing works
Distinction and Relationship between Elongation Rate and Processivity of RNA Polymerase II In Vivo
Mason, P. B., & Struhl, K. (2005) · Molecular Cell · doi:10.1016/j.molcel.2005.02.017 · Cited by 359 · see citing works
Single-Molecule Study of Transcriptional Pausing and Arrest by E. coli RNA Polymerase
John, R., Davenport, Wuite, G. J. L., Landick, R. C., & Bustamante, C. (2000) · Science · doi:10.1126/science.287.5462.2497 · Cited by 342 · see citing works
Bregman, D. B., Du, L., van der Zee, S. A., & Warren, S. L. (1995) · The Journal of Cell Biology · doi:10.1083/jcb.129.2.287 · Cited by 328 · see citing works
Listerman, I., Sapra, A. K., & Neugebauer, K. M. (2006) · Nature Structural & Molecular Biology · doi:10.1038/nsmb1135 · Cited by 309 · see citing works
Jackson, D. A., Iborra, F. J., Manders, E. M. M., & Cook, P. R. (1998) · Molecular Biology of the Cell · doi:10.1091/mbc.9.6.1523 · Cited by 292 · see citing works
The transcription cycle of RNA polymerase II in living cells
Kimurâ, H., Sugaya, K., & Cook, P. R. (2002) · The Journal of Cell Biology · doi:10.1083/jcb.200206019 · Cited by 264 · see citing works
Kinetic modelling approaches to in vivo imaging
Phair, R. D., & Misteli, T. (2001) · Nature Reviews Molecular Cell Biology · doi:10.1038/35103000 · Cited by 262 · see citing works
Self-citationIn vivo degradation of RNA polymerase II largest subunit triggered by alpha-amanitin
Nguyen, V. T. (1996) · Nucleic Acids Research · doi:10.1093/nar/24.15.2924 · Cited by 257 · see citing works
Transcriptional arrest within the first exon is a fast control mechanism in c-myc gene expression
Eick, D., & Bornkamm, G. W. (1986) · Nucleic Acids Research · doi:10.1093/nar/14.21.8331 · Cited by 253 · see citing works
The RNA Polymerase II Elongation Complex
Shilatifard, A., Conaway, R. C., & Conaway, J. W. (2003) · Annual Review of Biochemistry · doi:10.1146/annurev.biochem.72.121801.161551 · Cited by 253 · see citing works
The regulatory roles and mechanism of transcriptional pausing
Landick, R. C. (2006) · Biochemical Society Transactions · doi:10.1042/bst0341062 · Cited by 251 · see citing works
Investigating RNA polymerase II carboxyl‐terminal domain (CTD) phosphorylation
Palancade, B., & Bensaude, O. (2003) · European Journal of Biochemistry · doi:10.1046/j.1432-1033.2003.03794.x · Cited by 250 · see citing works
Cooperation Between RNA Polymerase Molecules in Transcription Elongation
Epshtein, V., & Nudler, E. (2003) · Science · doi:10.1126/science.1083219 · Cited by 234 · see citing works
Krumm, A., Hickey, L. B., & Groudine, M. T. (1995) · Genes & Development · doi:10.1101/gad.9.5.559 · Cited by 229 · see citing works
Dynamic behavior of transcription factors on a natural promoter in living cells
Becker, M., Baumann, C. T., John, S., Walker, D. A., Vigneron, M., McNally, J. G., & Hager, G. L. (2002) · EMBO Reports · doi:10.1093/embo-reports/kvf244 · Cited by 211 · see citing works
Single molecule analysis of RNA polymerase elongation reveals uniform kinetic behavior
Adelman, K., Porta, A. L., Santangelo, T. J., Lis, J. T., Roberts, J. W., & Wang, M. D. (2002) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.212358999 · Cited by 204 · see citing works
Three transitions in the RNA polymerase II transcription complex during initiation
Holstege, F. C. P., Fiedler, U., & Timmers, H. T. M. (1997) · The EMBO Journal · doi:10.1093/emboj/16.24.7468 · Cited by 193 · see citing works
Dynamics of heat shock factor association with native gene loci in living cells
Yao, J., Munson, K. M., Webb, W. W., & Lis, J. T. (2006) · Nature · doi:10.1038/nature05025 · Cited by 192 · see citing works
Stepwise RNP assembly at the site of H/ACA RNA transcription in human cells
Darzacq, X., Kittur, N. D., Roy, S., Shav‐Tal, Y., Singer, R. H., & Meier, U. T. (2006) · The Journal of Cell Biology · doi:10.1083/jcb.200601105 · Cited by 187 · see citing works
Self-citationDissecting the Contribution of Diffusion and Interactions to the Mobility of Nuclear Proteins
Beaudouin, J., Mora‐Bermúdez, F., Klee, T., Daigle, N., & Ellenberg, J. (2005) · Biophysical Journal · doi:10.1529/biophysj.105.071241 · Cited by 175 · see citing works
Rapid Changes in Drosophila Transcription After an Instantaneous Heat Shock
O’Brien, T., & Lis, J. T. (1993) · Molecular and Cellular Biology · doi:10.1128/mcb.13.6.3456 · Cited by 163 · see citing works
Structural basis of eukaryotic gene transcription
Boeger, H., Bushnell, D. A., Davis, R., Griesenbeck, J., Lorch, Y., Strattan, J. S., Westover, K. D., & Kornberg, R. D. (2005) · FEBS Letters · doi:10.1016/j.febslet.2004.11.027 · Cited by 151 · see citing works
Visualization of RNA Synthesis on Chromosomes
Miller, O. L., & Hamkalo, B. A. (1972) · International review of cytology · doi:10.1016/s0074-7696(08)61446-1 · Cited by 143 · see citing works
Margeat, E., Kapanidis, A. N., Tinnefeld, P., et al. (2005) · Biophysical Journal · doi:10.1529/biophysj.105.069252 · Cited by 142 · see citing works
Transcriptional Consequences of Topoisomerase Inhibition
Collins, I. J., Weber, A., & Levens, D. (2001) · Molecular and Cellular Biology · doi:10.1128/mcb.21.24.8437-8451.2001 · Cited by 121 · see citing works
Imaging gene expression in single living cells
Shav‐Tal, Y., Singer, R. H., & Darzacq, X. (2004) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm1494 · Cited by 104 · see citing works
Self-citationSprague, B. L., Mueller, F., Pego, R. L., Bungay, P. M., Stavreva, D. A., & McNally, J. G. (2006) · Biophysical Journal · doi:10.1529/biophysj.105.073676 · Cited by 104 · see citing works
RNA polymerase II structure: from core to functional complexes
Cramer, P. (2004) · Current Opinion in Genetics & Development · doi:10.1016/j.gde.2004.01.003 · Cited by 94 · see citing works
Promoter escape by RNA polymerase II
Dvir, A. (2002) · Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression · doi:10.1016/s0167-4781(02)00453-0 · Cited by 83 · see citing works
Interplay between positive and negative elongation factors: drawing a new view of DRB
Yamaguchi, Y., Wada, T., & Handa, H. (1998) · Genes to Cells · doi:10.1046/j.1365-2443.1998.00162.x · Cited by 81 · see citing works
Cheng, C., & Sharp, P. A. (2003) · Molecular and Cellular Biology · doi:10.1128/mcb.23.6.1961-1967.2003 · Cited by 81 · see citing works
[13] Visualization of single molecules of mRNAin Situ
Femino, A. M., Fogarty, K. E., Lifshitz, L. M., Carrington, W. A., & Singer, R. H. (2003) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/s0076-6879(03)61015-3 · Cited by 76 · see citing works
Self-citationBonnet, F., Vigneron, M., Bensaude, O., & Dubois, M. (1999) · Nucleic Acids Research · doi:10.1093/nar/27.22.4399 · Cited by 60 · see citing works
[33] Sensitive and high-resolution detection of RNA in situ
Chartrand, P., Bertrand, É., Singer, R. H., & Long, R. M. (2000) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/s0076-6879(00)18072-3 · Cited by 56 · see citing works
Self-citationDifferent populations of RNA polymerase II in living mammalian cells
Hieda, M., Winstanley, H. F., Maini, P. K., Iborra, F. J., & Cook, P. R. (2005) · Chromosome Research · doi:10.1007/s10577-005-7720-1 · Cited by 53 · see citing works
A Ribonucleolytic Rat Torpedoes RNA Polymerase II
Luo, W., & Bentley, D. L. (2004) · Cell · doi:10.1016/j.cell.2004.11.041 · Cited by 52 · see citing works
Efficient and selective initiation by yeast RNA polymerase B in a dinucleotide-primed reaction
Lescure, B., Williamson, V. M., & Sentenac, A. (1981) · Nucleic Acids Research · doi:10.1093/nar/9.1.31 · Cited by 50 · see citing works
Dynamics of transcription and mRNA export
Darzacq, X., Singer, R. H., & Shav‐Tal, Y. (2005) · Current Opinion in Cell Biology · doi:10.1016/j.ceb.2005.04.004 · Cited by 50 · see citing works
Self-citationRapid Changes in Drosophila Transcription After an Instantaneous Heat Shock
O’Brien, T., & Lis, J. T. (1993) · Molecular and Cellular Biology · doi:10.1128/mcb.13.6.3456-3463.1993 · Cited by 48 · see citing works
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- Authors
- Darzacq, X., Shav-Tal, Y., de Turris, V., Brody, Y., Shenoy, S. M., Phair, R. D., & Singer, R. H.
- Affiliations
- Albert Einstein College of Medicine (US)
- Bar-Ilan University (IL)
- Corresponding author
- Singer, Robert H
- Venue
- Nature Structural & Molecular Biology
- Published
- 2007-08-05
- Volume
- 14
- Issue
- 9
- Pages
- 796-806
- ISSN
- 1545-9985 (ISSN-L)
- 1545-9993
- PMC
- PMC4942130
- OpenAlex
- W1964259728
- Cited by
- 665
- References
- 62
- Citations by year
- 2026: 16 · 2025: 22 · 2024: 28 · 2023: 22 · 2022: 28 · 2021: 35 · 2020: 45 · 2019: 35 · 2018: 35 · 2017: 22 · 2016: 34 · 2015: 34 · 2014: 48 · 2013: 43 · 2012: 39
- FWCI
- 16.81
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Nature Structural & Molecular Biology (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- Funders
- National Institute of Biomedical Imaging and Bioengineering (R01EB002060, EB-002060)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gene Regulatory Network Analysis
- Keywords
- RNA polymerase II, transcription kinetics, transcription initiation, transcription elongation, promoter binding, transcriptional pausing, nascent mRNA, photoactivation, photobleaching, single-cell imaging, kinetic model, transcriptional regulation
- MeSH terms
- Base Sequence, Humans, Kinetics, Phosphorylation, Photochemistry, RNA Polymerase II, Transcription, Genetic, In Situ Hybridization, Fluorescence, DNA Primers, Cell Line, Tumor
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