The DOI matches its Crossref record.
Design and Chemical Synthesis of a Homogeneous Polymer-Modified Erythropoiesis Protein
Abstract
We report the design and total chemical synthesis of “synthetic erythropoiesis protein” (SEP), a 51-kilodalton protein-polymer construct consisting of a 166-amino–acid polypeptide chain and two covalently attached, branched, and monodisperse polymer moieties that are negatively charged. The ability to control the chemistry allowed us to synthesize a macromolecule of precisely defined covalent structure. SEP was homogeneous as shown by high-resolution analytical techniques, with a mass of 50,825 ±10 daltons by electrospray mass spectrometry, and with a pI of 5.0. In cell and animal assays for erythropoiesis, SEP displayed potent biological activity and had significantly prolonged duration of action in vivo. These chemical methods are a powerful tool in the rational design of protein constructs with potential therapeutic applications.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cited by
Adding New Chemistries to the Genetic Code
Liu, C. C., & Schultz, P. G. (2010) · Annual Review of Biochemistry · doi:10.1146/annurev.biochem.052308.105824 · Cited by 1,725
Hydrolytic Stability of Hydrazones and Oximes
Kalia, J., & Raines, R. T. (2008) · Angewandte Chemie International Edition · doi:10.1002/anie.200802651 · Cited by 916
Native Chemical Ligation and Extended Methods: Mechanisms, Catalysis, Scope, and Limitations
Agouridas, V., El Mahdi, O., Diemer, V., Cargoët, M., Monbaliu, J.-C. M., & Melnyk, O. (2019) · Chemical Reviews · doi:10.1021/acs.chemrev.8b00712 · Cited by 650
Therapeutic Protein–Polymer Conjugates: Advancing Beyond PEGylation
Pelegri-O’Day, E. M., Lin, E.-W., & Maynard, H. D. (2014) · Journal of the American Chemical Society · doi:10.1021/ja504390x · Cited by 622
Nanotechnology for protein delivery: Overview and perspectives
Yu, M., Wu, J., Shi, J., & Farokhzad, O. C. (2016) · Journal of Controlled Release · doi:10.1016/j.jconrel.2015.10.012 · Cited by 410
Metadata
- Authors
- Kochendoerfer, G. G., Chen, S.-Y., Mao, F., Cressman, S., Traviglia, S., Shao, H., Hunter, C. L., Low, D. W., Cagle, E. N., Carnevali, M., Gueriguian, V., Keogh, P. J., Porter, H., Stratton, S. M., Wiedeke, M. C., Wilken, J., Tang, J., Levy, J. J., Miranda, L. P., Crnogorac, M. M., Kalbag, S., Botti, P., Schindler-Horvat, J., Savatski, L., Adamson, J. W., Kung, A., Kent, S. B. H., & Bradburne, J. A.
- Venue
- Science
- Published
- 2003-02-07
- Volume
- 299
- Issue
- 5608
- Pages
- 884-887
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2102893844
- Cited by
- 312
- References
- 0
- FWCI
- 11.35
- Type
- journal article
- Language
- en
- Open access
- closed
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Design and Chemical Synthesis of a Homogeneous Polymer-Modified Erythropoiesis Protein. https://app.cochat.ai/papers/10.1126/science.1079085
- The paper
- Kochendoerfer, G. G., Chen, S.-Y., Mao, F., Cressman, S., Traviglia, S., Shao, H., Hunter, C. L., Low, D. W., Cagle, E. N., Carnevali, M., Gueriguian, V., Keogh, P. J., Porter, H., Stratton, S. M., Wiedeke, M. C., Wilken, J., Tang, J., Levy, J. J., Miranda, L. P., . . . Bradburne, J. A. (2003). Design and Chemical Synthesis of a Homogeneous Polymer-Modified Erythropoiesis Protein. Science. https://doi.org/10.1126/science.1079085
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
