The DOI matches its Crossref record; 22 of the 22 references it cites were found in a registry or an index.
Protein Insertion into the Mitochondrial Inner Membrane by a Twin-Pore Translocase
Abstract
The mitochondrial inner membrane imports numerous proteins that span it multiple times using the membrane potential Δψ as the only external energy source. We purified the protein insertion complex (TIM22 complex), a twin-pore translocase that mediated the insertion of precursor proteins in a three-step process. After the precursor is tethered to the translocase without losing energy from the Δψ, two energy-requiring steps were needed. First, Δψ acted on the precursor protein and promoted its docking in the translocase complex. Then, Δψ and an internal signal peptide together induced rapid gating transitions in one pore and closing of the other pore and drove membrane insertion to completion. Thus, protein insertion was driven by the coordinated action of a twin-pore complex in two voltage-dependent steps.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Tom40 forms the hydrophilic channel of the mitochondrial import pore for preproteins
Hill, K., Model, K., Ryan, M. T., Dietmeier, K., Martin, F., Wagner, R., & Pfanner, N. (1998) · Nature · doi:10.1038/26780 · Cited by 512
Self-citationVersatility of the mitochondrial protein import machinery
Pfanner, N., & Geissler, A. (2001) · Nature Reviews Molecular Cell Biology · doi:10.1038/35073006 · Cited by 505
Self-citationThe Preprotein Translocation Channel of the Outer Membrane of Mitochondria
Künkele, K.-P., Heins, S., Dembowski, M., Nargang, F. E., Benz, R., Thieffry, M., Walz, J., Lill, R., Nussberger, S., & Neupert, W. (1998) · Cell · doi:10.1016/s0092-8674(00)81206-4 · Cited by 398
Import of carrier proteins into the mitochondrial inner membrane mediated by Tim22
Sirrenberg, C., Bauer, M. F., Guiard, B., Neupert, W., & Brunner, M. (1996) · Nature · doi:10.1038/384582a0 · Cited by 342
Import of Mitochondrial Carriers Mediated by Essential Proteins of the Intermembrane Space
Koehler, C. M., Jarosch, E., Tokatlidis, K., Schmid, K., Schweyen, R. J., & Schatz, G. (1998) · Science · doi:10.1126/science.279.5349.369 · Cited by 326
Pfanner, N., Tropschug, M., & Neupert, W. (1987) · Cell · doi:10.1016/0092-8674(87)90619-2 · Cited by 319
Self-citationSirrenberg, C., Endres, M., Fölsch, H., Stuart, R. A., Neupert, W., & Brunner, M. (1998) · Nature · doi:10.1038/36136 · Cited by 299
Brix, J., Rüdiger, S., Bukau, B., Schneider-Mergener, J., & Pfanner, N. (1999) · Journal of Biological Chemistry · doi:10.1074/jbc.274.23.16522 · Cited by 249
Self-citationProtein translocation into mitochondria: the role of TIM complexes
Bauer, M. F., Hofmann, S., Neupert, W., & Brunner, M. (2000) · Trends in Cell Biology · doi:10.1016/s0962-8924(99)01684-0 · Cited by 238
Pfanner, N., & Neupert, W. (1985) · The EMBO Journal · doi:10.1002/j.1460-2075.1985.tb04009.x · Cited by 236
Self-citation
Cited by
Mitochondrial proteins: from biogenesis to functional networks
Pfanner, N., Warscheid, B., & Wiedemann, N. (2019) · Nature Reviews Molecular Cell Biology · doi:10.1038/s41580-018-0092-0 · Cited by 1,028
Self-citationProtein translocation across the eukaryotic endoplasmic reticulum and bacterial plasma membranes.
Rapoport, T. A. (2007) · Nature · doi:10.1038/nature06384 · Cited by 977
Mitochondrial protein import: from proteomics to functional mechanisms
Schmidt, O., Pfanner, N., & Meisinger, C. (2010) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm2959 · Cited by 717
Self-citationThe Protein Import Machinery of Mitochondria
Wiedemann, N., Frazier, A. E., & Pfanner, N. (2004) · Journal of Biological Chemistry · doi:10.1074/jbc.r400003200 · Cited by 336
Self-citationFunction of Cytosolic Chaperones in Tom70-Mediated Mitochondrial Import
C.Y. Fan, A., & C. Young, J. (2011) · Protein & Peptide Letters · doi:10.2174/092986611794475020 · Cited by 64
Metadata
- Authors
- Rehling, P., Model, K., Brandner, K., Kovermann, P., Sickmann, A., Meyer, H. E., Kühlbrandt, W., Wagner, R., Truscott, K. N., & Pfanner, N.
- Venue
- Science
- Published
- 2003-03-14
- Volume
- 299
- Issue
- 5613
- Pages
- 1747-1751
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W1967092231
- Cited by
- 332
- References
- 22
- FWCI
- 7.29
- Type
- journal article
- Language
- en
- Open access
- closed
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Protein Insertion into the Mitochondrial Inner Membrane by a Twin-Pore Translocase. https://app.cochat.ai/papers/10.1126/science.1080945
- The paper
- Rehling, P., Model, K., Brandner, K., Kovermann, P., Sickmann, A., Meyer, H. E., Kühlbrandt, W., Wagner, R., Truscott, K. N., & Pfanner, N. (2003). Protein Insertion into the Mitochondrial Inner Membrane by a Twin-Pore Translocase. Science. https://doi.org/10.1126/science.1080945
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
