The DOI matches its Crossref record; 20 of the 20 references it cites were found in a registry or an index.
Single-Molecule Measurement of Protein Folding Kinetics
Abstract
In order to investigate the behavior of single molecules under conditions far from equilibrium, we have coupled a microfabricated laminar-flow mixer to a confocal optical system. This combination enables time-resolved measurement of Förster resonance energy transfer after an abrupt change in solution conditions. Observations of a small protein show the evolution of the intramolecular distance distribution as folding progresses. This technique can expose subpopulations, such as unfolded protein under conditions favoring the native structure, that would be obscured in equilibrium experiments.
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Cites
Fluorescence Spectroscopy of Single Biomolecules
Weiss, S. (1999) · Science · doi:10.1126/science.283.5408.1676 · Cited by 2,046
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Probing the free-energy surface for protein folding with single-molecule fluorescence spectroscopy
Schuler, B., Lipman, E. A., & Eaton, W. A. (2002) · Nature · doi:10.1038/nature01060 · Cited by 921
Self-citationA Single-Molecule Study of RNA Catalysis and Folding
Zhuang, X., Bartley, L. E., Babcock, H. P., Russell, R., Ha, T., Herschlag, D., & Chu, S. (2000) · Science · doi:10.1126/science.288.5473.2048 · Cited by 732
Introduction to Polymer Physics
Doi, M., & See, H. (1995) · doi:10.1093/oso/9780198517726.001.0001 · Cited by 728
Resonance Energy Transfer: Theory and Data
van der Meer, B. W., Coker, E. G., & Chen, S.-Y. S. (1994) · Cited by 679
Deniz, A. A., Dahan, M., Grunwell, J. R., Ha, T., Faulhaber, A. E., Chemla, D. S., Weiss, S., & Schultz, P. G. (1999) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.96.7.3670 · Cited by 547
Correlating Structural Dynamics and Function in Single Ribozyme Molecules
Zhuang, X., Kim, H., Pereira, M. J. B., Babcock, H. P., Walter, N. G., & Chu, S. (2002) · Science · doi:10.1126/science.1069013 · Cited by 512
Deniz, A. A., Laurence, T. A., Beligere, G. S., Dahan, M., Martin, A. B., Chemla, D. S., Dawson, P. E., Schultz, P. G., & Weiss, S. (2000) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.090104997 · Cited by 469
Watching proteins fold one molecule at a time
Rhoades, E., Gussakovsky, E., & Haran, G. (2003) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.2628068100 · Cited by 340
Cited by
Statistical Kinetics of Macromolecular Dynamics
Shaevitz, J. W., Block, S. M., & Schnitzer, M. J. (2005) · Biophysical Journal · doi:10.1529/biophysj.105.064295 · Cited by 86
Metadata
- Authors
- Lipman, E. A., Schuler, B., Bakajin, O., & Eaton, W. A.
- Venue
- Science
- Published
- 2003-08-29
- Volume
- 301
- Issue
- 5637
- Pages
- 1233-1235
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2149756514
- Cited by
- 401
- References
- 20
- FWCI
- 11.04
- Type
- journal article
- Language
- en
- Open access
- closed
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