The DOI matches its Crossref record; 28 of the 28 references it cites were found in a registry or an index.
Tracking FACT and the RNA Polymerase II Elongation Complex Through Chromatin in Vivo
Abstract
RNA polymerase II (Pol II) transcription through nucleosomes is facilitated in vitro by the protein complex FACT ( Fa cilitates C hromatin T ranscription). Here we show that FACT is associated with actively transcribed Pol II genes on Drosophila polytene chromosomes. FACT displays kinetics of recruitment and of chromosome tracking in vivo similar to Pol II and elongation factors Spt5 and Spt6. Interestingly, FACT does not colocalize with Pol III–transcribed genes, which are known to undergo nucleosome transfer rather than disassembly in vitro. Our observations are consistent with FACT being restricted to transcription that involves nucleosome disassembly mechanisms.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
The 5′ ends of Drosophila heat shock genes in chromatin are hypersensitive to DNase I
Wu, C. (1980) · Nature · doi:10.1038/286854a0 · Cited by 1,097
FACT Facilitates Transcription-Dependent Nucleosome Alteration
Belotserkovskaya, R., Oh, S., Bondarenko, V. A., Orphanides, G., Studitsky, V. M., & Reinberg, D. (2003) · Science · doi:10.1126/science.1085703 · Cited by 868
Self-citationRougvie, A. E., & Lis, J. T. (1988) · Cell · doi:10.1016/s0092-8674(88)91087-2 · Cited by 666
Self-citationTsukiyama, T., Becker, P. B., & Wu, C. (1994) · Nature · doi:10.1038/367525a0 · Cited by 664
FACT, a Factor that Facilitates Transcript Elongation through Nucleosomes
Orphanides, G., LeRoy, G., Chang, C.-H., Luse, D. S., & Reinberg, D. (1998) · Cell · doi:10.1016/s0092-8674(00)80903-4 · Cited by 656
Self-citationThe chromatin-specific transcription elongation factor FACT comprises human SPT16 and SSRP1 proteins
Orphanides, G., Wu, W.-H., Lane, W. S., Hampsey, M., & Reinberg, D. (1999) · Nature · doi:10.1038/22350 · Cited by 554
Self-citationRNA Polymerase II Elongation Factors of Saccharomyces cerevisiae: a Targeted Proteomics Approach
Krogan, N. J., Kim, M., Ahn, S. H., Zhong, G., Kobor, M. S., Cagney, G., Emili, A., Shilatifard, A., Buratowski, S., & Greenblatt, J. F. (2002) · Molecular and Cellular Biology · doi:10.1128/mcb.22.20.6979-6992.2002 · Cited by 497
Hartzog, G. A., Wada, T., Handa, H., & Winston, F. (1998) · Genes & Development · doi:10.1101/gad.12.3.357 · Cited by 461
Nucleosome Remodeling Induced by RNA Polymerase II
Kireeva, M. L., Walter, W., Tchernajenko, V., Bondarenko, V., Kashlev, M., & Studitsky, V. M. (2002) · Molecular Cell · doi:10.1016/s1097-2765(02)00472-0 · Cited by 440
Deuring, R., Fanti, L., Armstrong, J. A., Sarte, M., Papoulas, O., Prestel, M., Daubresse, G., Verardo, M., Moseley, S. L., Berloco, M., et al. (2000) · Molecular Cell · doi:10.1016/s1097-2765(00)80430-x · Cited by 385
Cited by
Cellular processing of platinum anticancer drugs
Wang, D., & Lippard, S. J. (2005) · Nature Reviews Drug Discovery · doi:10.1038/nrd1691 · Cited by 3,801
Functions of Site-Specific Histone Acetylation and Deacetylation
Shahbazian, M. D., & Grunstein, M. (2007) · Annual Review of Biochemistry · doi:10.1146/annurev.biochem.76.052705.162114 · Cited by 1,635
Proteotoxic stress and inducible chaperone networks in neurodegenerative disease and aging
Morimoto, R. I. (2008) · Genes & Development · doi:10.1101/gad.1657108 · Cited by 872
Histones: annotating chromatin
Campos, E. I., & Reinberg, D. (2009) · Annual Review of Genetics · doi:10.1146/annurev.genet.032608.103928 · Cited by 852
Self-citationRegulation of H SF 1 Function in the Heat Stress Response: Implications in Aging and Disease
Anckar, J., & Sistonen, L. (2011) · Annual Review of Biochemistry · doi:10.1146/annurev-biochem-060809-095203 · Cited by 773
Metadata
- Authors
- Saunders, A., Werner, J., Andrulis, E. D., Nakayama, T., Hirose, S., Reinberg, D., & Lis, J. T.
- Venue
- Science
- Published
- 2003-08-22
- Volume
- 301
- Issue
- 5636
- Pages
- 1094-1096
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W1971196679
- Cited by
- 289
- References
- 28
- FWCI
- 8.26
- Type
- journal article
- Language
- en
- Open access
- closed
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Tracking FACT and the RNA Polymerase II Elongation Complex Through Chromatin in Vivo. https://app.cochat.ai/papers/10.1126/science.1085712
- The paper
- Saunders, A., Werner, J., Andrulis, E. D., Nakayama, T., Hirose, S., Reinberg, D., & Lis, J. T. (2003). Tracking FACT and the RNA Polymerase II Elongation Complex Through Chromatin in Vivo. Science. https://doi.org/10.1126/science.1085712
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
