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Protein Conformational Dynamics Probed by Single-Molecule Electron Transfer
Abstract
Electron transfer is used as a probe for angstrom-scale structural changes in single protein molecules. In a flavin reductase, the fluorescence of flavin is quenched by a nearby tyrosine residue by means of photo-induced electron transfer. By probing the fluorescence lifetime of the single flavin on a photon-by-photon basis, we were able to observe the variation of flavin-tyrosine distance over time. We could then determine the potential of mean force between the flavin and the tyrosine, and a correlation analysis revealed conformational fluctuation at multiple time scales spanning from hundreds of microseconds to seconds. This phenomenon suggests the existence of multiple interconverting conformers related to the fluctuating catalytic reactivity.
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Citations
Cited by
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Henzler-Wildman, K., & Kern, D. (2007) · Nature · doi:10.1038/nature06522 · Cited by 2,545
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McKinney, S. A., Joo, C., & Ha, T. (2006) · Biophysical Journal · doi:10.1529/biophysj.106.082487 · Cited by 760
Power-law intermittency of single emitters
CICHOS, F., VONBORCZYSKOWSKI, C., & ORRIT, M. (2007) · Current Opinion in Colloid & Interface Science · doi:10.1016/j.cocis.2007.07.012 · Cited by 301
(2006) · Principles of Fluorescence Spectroscopy · doi:10.1007/978-0-387-46312-4_8 · Cited by 278
Labeling and Single-Molecule Methods To Monitor G Protein-Coupled Receptor Dynamics
Tian, H., Fürstenberg, A., & Huber, T. (2017) · Chemical Reviews · doi:10.1021/acs.chemrev.6b00084 · Cited by 114
Metadata
- Authors
- Yang, H., Luo, G., Karnchanaphanurach, P., Louie, T.-M., Rech, I., Cova, S., Xun, L., & Xie, X. S.
- Venue
- Science
- Published
- 2003-10-10
- Volume
- 302
- Issue
- 5643
- Pages
- 262-266
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2031573489
- Cited by
- 843
- References
- 0
- FWCI
- 16.51
- Type
- journal article
- Language
- en
- Open access
- closed
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- Yang, H., Luo, G., Karnchanaphanurach, P., Louie, T.-M., Rech, I., Cova, S., Xun, L., & Xie, X. S. (2003). Protein Conformational Dynamics Probed by Single-Molecule Electron Transfer. Science. https://doi.org/10.1126/science.1086911
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