High Deleterious Genomic Mutation Rate in Stationary Phase of Escherichia coli
Open accessAbstract
In natural habitats, bacteria spend most of their time in some form of growth arrest. Little is known about deleterious mutations in such stages, and consequently there is limited understanding of what evolutionary events occur. In a deleterious mutation accumulation experiment in prolonged stationary phase of Escherichia coli , about 0.03 slightly deleterious mutations were observed per genome per day. This is over an order of magnitude higher than extrapolations from fast-growing cells, but in line with inferences from observations in adaptive stationary phase mutation experiments. These findings may affect understanding of bacterial evolution and the emergence of bacterial pathogenicity.
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Mutations in carbon source metabolism-associated genes such as lacZ can occur after plating due to adaptive mutagenesis, because the selective agent does not immediately kill the non-mutant cells.
“Mutations in carbon source metabolism-associated genes such as lacZ can occur after plating due to adaptive mutagenesis, because the selective agent does not immediately kill the non-mutant cells [18,19].”
From Efficient, robust, and versatile fluctuation data analysis using MLE MUtation Rate calculator (mlemur) (2023) · cites this paper as [18]
The passage labeled Abstract (page unavailable) describes a Lac frameshift reversion assay in which starving E. coli can acquire adaptive mutations that permit growth on an alternative nutrient. It does not specify lacZ, establish that the mutations occur after plating, or say that the selective agent does not immediately kill non-mutant cells. (LLM, conf=0.84)
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- Authors
- Loewe, L., Textor, V., & Scherer, S.
- Affiliations
- Technical University of Munich (DE)
- Corresponding author
- Loewe, Laurence
- Venue
- Science
- Published
- 2003-11-28
- Volume
- 302
- Issue
- 5650
- Pages
- 1558-1560
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2011836722
- Cited by
- 85
- References
- 2
- Citations by year
- 2026: 1 · 2025: 2 · 2024: 2 · 2023: 3 · 2022: 3 · 2021: 1 · 2020: 6 · 2019: 4 · 2018: 1 · 2017: 2 · 2016: 6 · 2015: 1 · 2014: 3 · 2013: 1 · 2012: 6
- FWCI
- 3.52
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Science (publishedVersion)
- PubMed (publishedVersion)
- ERA (submittedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Evolution and Genetic Dynamics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Phylogenetic Studies
- Keywords
- deleterious mutations, mutation rate, Escherichia coli, stationary phase, growth arrest, mutation accumulation experiment, bacterial evolution, bacterial pathogenicity
- MeSH terms
- Escherichia coli, Biological Evolution, Mutation, Phenotype, Selection, Genetic, Temperature, Time Factors, Likelihood Functions, Genome, Bacterial
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