Cited by 460Cellular transport and secretionRNA and protein synthesis mechanismsNuclear Structure and Function

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Protein Translocation Across Biological Membranes

Wickner, William, Schekman, Randy·2005·Science

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Abstract

Subcellular compartments have unique protein compositions, yet protein synthesis only occurs in the cytosol and in mitochondria and chloroplasts. How do proteins get where they need to go? The first steps are targeting to an organelle and efficient translocation across its limiting membrane. Given that most transport systems are exquisitely substrate specific, how are diverse protein sequences recognized for translocation? Are they translocated as linear polypeptide chains or after folding? During translocation, how are diverse amino acyl side chains accommodated? What are the proteins and the lipid environment that catalyze transport and couple it to energy? How is translocation coordinated with protein synthesis and folding, and how are partially translocated transmembrane proteins released into the lipid bilayer? We review here the marked progress of the past 35 years and salient questions for future work. Subcellular compartments have unique protein compositions, yet protein synthesis only occurs in the cytosol and in mitochondria and chloroplasts. How do proteins get where they need to go? The first steps are targeting to an organelle and efficient translocation across its limiting membrane. Given that most transport systems are exquisitely substrate specific, how are diverse protein sequences recognized for translocation? Are they translocated as linear polypeptide chains or after folding? During translocation, how are diverse amino acyl side chains accommodated? What are the proteins and the lipid environment that catalyze transport and couple it to energy? How is translocation coordinated with protein synthesis and folding, and how are partially translocated transmembrane proteins released into the lipid bilayer? We review here the marked progress of the past 35 years and salient questions for future work.

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Authors: Wickner, W., & Schekman, R.
Affiliations: Dartmouth College (US)
Corresponding author: Wickner, William
Schekman, Randy
ORCID: Wickner, William
Schekman, Randy
Venue: Science
Publisher: American Association for the Advancement of Science (AAAS)
Published: 2005-12-02
Volume: 310
Issue: 5753
Pages: 1452-1456
ISSN: 0036-8075 (ISSN-L)
1095-9203
DOI: 10.1126/science.1113752
OpenAlex: W2021288400
Cited by: 458
References: 96
Citations by year: 2026: 7 · 2025: 13 · 2024: 10 · 2023: 13 · 2022: 20 · 2021: 20 · 2020: 25 · 2019: 13 · 2018: 13 · 2017: 16 · 2016: 18 · 2015: 18 · 2014: 35 · 2013: 19 · 2012: 28
FWCI: 2.36
Type: journal article
Language: en
Open access: closed
Locations: Science (publishedVersion)
PubMed (publishedVersion)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Nuclear Structure and Function
Keywords: protein translocation, biological membranes, organelle targeting, mitochondrial protein import, chloroplast protein import, substrate specificity, membrane lipid environment, energy coupling, subcellular compartmentalization
MeSH terms: Carrier Proteins, Endoplasmic Reticulum, Intracellular Membranes, Mitochondria, Protein Biosynthesis, Protein Folding, Peroxisomes, Protein Transport

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Protein Translocation Across Biological Membranes

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