The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.
Evidence for Macromolecular Protein Rings in the Absence of Bulk Water
Abstract
We have examined the architecture of a protein complex in the absence of bulk water. By determining collision cross sections of assemblies of the trp RNA binding protein, TRAP, we established that the 11-membered ring topology of the complex can be maintained within a mass spectrometer. We also found that the binding of tryptophan enhances the stability of the ring structure and that addition of a specific RNA molecule increases the size of the complex and prevents structural collapse. These results provide definitive evidence that protein quaternary structure can be maintained in the absence of bulk water and highlight the potential of ion mobility separation for defining shapes of heterogeneous macromolecular assemblies.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Electrospray Ionization for Mass Spectrometry of Large Biomolecules
Fenn, J. B., Mann, M., Meng, C. K., Wong, S. F., & Whitehouse, C. M. (1989) · Science · doi:10.1126/science.2675315 · Cited by 7,634
Principles of protein-protein interactions
Jones, S., & Thornton, J. M. (1996) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.93.1.13 · Cited by 2,684
Cited by
Lanucara, F., Holman, S. W., Gray, C. J., & Eyers, C. E. (2014) · Nature Chemistry · doi:10.1038/nchem.1889 · Cited by 901
Native mass spectrometry: a bridge between interactomics and structural biology
Heck, A. J. R. (2008) · Nature Methods · doi:10.1038/nmeth.1265 · Cited by 766
Native Mass Spectrometry: What is in the Name?
Leney, A. C., & Heck, A. J. R. (2017) · Journal of the American Society for Mass Spectrometry · doi:10.1007/s13361-016-1545-3 · Cited by 602
Ion mobility mass spectrometry of proteins and proteinassemblies
Uetrecht, C., Rose, R. J., van Duijn, E., Lorenzen, K., & Heck, A. J. R. (2010) · Chem. Soc. Rev. · doi:10.1039/b914002f · Cited by 462
Lapthorn, C., Pullen, F., & Chowdhry, B. Z. (2013) · Mass Spectrometry Reviews · doi:10.1002/mas.21349 · Cited by 398
Metadata
- Authors
- Ruotolo, B. T., Giles, K., Campuzano, I., Sandercock, A. M., Bateman, R. H., & Robinson, C. V.
- Venue
- Science
- Published
- 2005-12-09
- Volume
- 310
- Issue
- 5754
- Pages
- 1658-1661
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2071884490
- Cited by
- 596
- References
- 2
- FWCI
- 10.55
- Type
- journal article
- Language
- en
- Open access
- closed
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Evidence for Macromolecular Protein Rings in the Absence of Bulk Water. https://app.cochat.ai/papers/10.1126/science.1120177
- The paper
- Ruotolo, B. T., Giles, K., Campuzano, I., Sandercock, A. M., Bateman, R. H., & Robinson, C. V. (2005). Evidence for Macromolecular Protein Rings in the Absence of Bulk Water. Science. https://doi.org/10.1126/science.1120177
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
