Cited by 825Microbial Metabolic Engineering and BioproductionFungal and yeast genetics researchBiofuel production and bioconversion

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Engineering Yeast Transcription Machinery for Improved Ethanol Tolerance and Production

Alper, Hal, Moxley, Joel, Nevoigt, Elke, Fink, Gerald R., Stephanopoulos, Gregory·2006·Science

DOI 10.1126/science.1131969

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Abstract

Global transcription machinery engineering (gTME) is an approach for reprogramming gene transcription to elicit cellular phenotypes important for technological applications. Here we show the application of gTME to Saccharomyces cerevisiae for improved glucose/ethanol tolerance, a key trait for many biofuels programs. Mutagenesis of the transcription factor Spt15p and selection led to dominant mutations that conferred increased tolerance and more efficient glucose conversion to ethanol. The desired phenotype results from the combined effect of three separate mutations in the SPT15 gene [serine substituted for phenylalanine (Phe 177 Ser) and, similarly, Tyr 195 His, and Lys 218 Arg]. Thus, gTME can provide a route to complex phenotypes that are not readily accessible by traditional methods.

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BackgroundInfluential
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Background

Authors: Alper, H., Moxley, J., Nevoigt, E., Fink, G. R., & Stephanopoulos, G.
Affiliations: Whitehead Institute for Biomedical Research (US)
Corresponding author: Stephanopoulos, Gregory
ORCID: Alper, Hal
Nevoigt, Elke
Fink, Gerald R.
Stephanopoulos, Gregory
Venue: Science
Publisher: American Association for the Advancement of Science (AAAS)
Published: 2006-12-08
Volume: 314
Issue: 5805
Pages: 1565-1568
ISSN: 0036-8075 (ISSN-L)
1095-9203
DOI: 10.1126/science.1131969
OpenAlex: W2080537888
Cited by: 823
References: 26
Citations by year: 2026: 10 · 2025: 23 · 2024: 31 · 2023: 18 · 2022: 28 · 2021: 39 · 2020: 43 · 2019: 37 · 2018: 39 · 2017: 44 · 2016: 49 · 2015: 61 · 2014: 55 · 2013: 57 · 2012: 68
FWCI: 14.67
Type: journal article
Language: en
Open access: closed
Locations: Science (publishedVersion)
PubMed (publishedVersion)
Funders: National Institute of General Medical Sciences (GM035010)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
Physical Sciences › Engineering › Biomedical Engineering › Biofuel production and bioconversion
Keywords: Saccharomyces cerevisiae, ethanol tolerance, glucose fermentation, transcription factor mutations, dominant mutations, biofuel production, yeast strain engineering
MeSH terms: Ethanol, Fermentation, Genetic Engineering, Glucose, Phenotype, Saccharomyces cerevisiae, Transcription Factors, Transcription, Genetic, Transformation, Genetic, Up-Regulation, Gene Expression Regulation, Fungal, Mutagenesis, Cell Cycle Proteins, Amino Acid Substitution, Gene Expression Profiling, Saccharomyces cerevisiae Proteins, TATA-Box Binding Protein, Transcription Factor TFIID, TATA-Binding Protein Associated Factors

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Engineering Yeast Transcription Machinery for Improved Ethanol Tolerance and Production

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