The DOI matches its Crossref record; 4 of the 4 references it cites were found in a registry or an index.
Multiple High-Throughput Analyses Monitor the Response of E. coli to Perturbations
Abstract
Analysis of cellular components at multiple levels of biological information can provide valuable functional insights. We performed multiple high-throughput measurements to study the response of Escherichia coli cells to genetic and environmental perturbations. Analysis of metabolic enzyme gene disruptants revealed unexpectedly small changes in messenger RNA and proteins for most disruptants. Overall, metabolite levels were also stable, reflecting the rerouting of fluxes in the metabolic network. In contrast, E. coli actively regulated enzyme levels to maintain a stable metabolic state in response to changes in growth rate. E. coli thus seems to use complementary strategies that result in a metabolic network robust against perturbations.
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Cites
Construction of Escherichia coli K‐12 in‐frame, single‐gene knockout mutants: the Keio collection
Baba, T., Ara, T., Hasegawa, M., Takai, Y., Okumura, Y., Baba, M., Datsenko, K. A., Tomita, M., Wanner, B. L., & Mori, H. (2006) · Molecular Systems Biology · doi:10.1038/msb4100050 · Cited by 7,858
Self-citationKitano, H. (2004) · Nature Reviews Genetics · doi:10.1038/nrg1471 · Cited by 2,278
Role of duplicate genes in genetic robustness against null mutations
Gu, Z., Steinmetz, L. M., Gu, X., Scharfe, C., Davis, R. W., & Li, W.-H. (2003) · Nature · doi:10.1038/nature01198 · Cited by 889
Metabolic network structure determines key aspects of functionality and regulation
Stelling, J., Klamt, S., Bettenbrock, K., Schuster, S., & Gilles, E. D. (2002) · Nature · doi:10.1038/nature01166 · Cited by 802
Cited by
Tuning gene expression to changing environments: from rapid responses to evolutionary adaptation
López-Maury, L., Marguerat, S., & Bähler, J. (2008) · Nature Reviews Genetics · doi:10.1038/nrg2398 · Cited by 1,061
The Moderately Efficient Enzyme: Evolutionary and Physicochemical Trends Shaping Enzyme Parameters
Bar-Even, A., Noor, E., Savir, Y., Liebermeister, W., Davidi, D., Tawfik, D. S., & Milo, R. (2011) · Biochemistry · doi:10.1021/bi2002289 · Cited by 1,050
Glycolytic strategy as a tradeoff between energy yield and protein cost
Flamholz, A., Noor, E., Bar-Even, A., Liebermeister, W., & Milo, R. (2013) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1215283110 · Cited by 531
Multidimensional Optimality of Microbial Metabolism
Schuetz, R., Zamboni, N., Zampieri, M., Heinemann, M., & Sauer, U. (2012) · Science · doi:10.1126/science.1216882 · Cited by 420
Rea, S. L., Ventura, N., & Johnson, T. E. (2007) · PLoS Biology · doi:10.1371/journal.pbio.0050259 · Cited by 383
Metadata
- Authors
- Ishii, N., Nakahigashi, K., Baba, T., Robert, M., Soga, T., Kanai, A., Hirasawa, T., Naba, M., Hirai, K., Hoque, A., Ho, P. Y., Kakazu, Y., Sugawara, K., Igarashi, S., Harada, S., Masuda, T., Sugiyama, N., Togashi, T., Hasegawa, M., Takai, Y., Yugi, K., Arakawa, K., Iwata, N., Toya, Y., Nakayama, Y., Nishioka, T., Shimizu, K., Mori, H., & Tomita, M.
- Venue
- Science
- Published
- 2007-04-27
- Volume
- 316
- Issue
- 5824
- Pages
- 593-597
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2141776893
- Cited by
- 674
- References
- 4
- FWCI
- 17.96
- Type
- journal article
- Language
- en
- Open access
- closed
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- Ishii, N., Nakahigashi, K., Baba, T., Robert, M., Soga, T., Kanai, A., Hirasawa, T., Naba, M., Hirai, K., Hoque, A., Ho, P. Y., Kakazu, Y., Sugawara, K., Igarashi, S., Harada, S., Masuda, T., Sugiyama, N., Togashi, T., Hasegawa, M., . . . Tomita, M. (2007). Multiple High-Throughput Analyses Monitor the Response of E. coli to Perturbations. Science. https://doi.org/10.1126/science.1132067
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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