The DOI matches its Crossref record; 3 of the 3 references it cites were found in a registry or an index.
Counting Low-Copy Number Proteins in a Single Cell
Abstract
We have designed a microfluidic device in which we can manipulate, lyse, label, separate, and quantify the protein contents of a single cell using single-molecule fluorescence counting. Generic labeling of proteins is achieved through fluorescent-antibody binding. The use of cylindrical optics enables high-efficiency (≈60%) counting of molecules in micrometer-sized channels. We used this microfluidic device to quantify β 2 adrenergic receptors expressed in insect cells (SF9). We also analyzed phycobiliprotein contents in individual cyanobacterial cells ( Synechococcus sp. PCC 7942) and observed marked differences in the levels of specific complexes in cell populations that were grown under nitrogen-depleted conditions.
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Cites
Single-cell proteomic analysis of S. cerevisiae reveals the architecture of biological noise
Newman, J. R. S., Ghaemmaghami, S., Ihmels, J., Breslow, D. K., Noble, M., DeRisi, J. L., & Weissman, J. S. (2006) · Nature · doi:10.1038/nature04785 · Cited by 1,483
Stochastic protein expression in individual cells at the single molecule level
Cai, L., Friedman, N., & Xie, X. S. (2006) · Nature · doi:10.1038/nature04599 · Cited by 1,145
Probing Gene Expression in Live Cells, One Protein Molecule at a Time
Yu, J., Xiao, J., Ren, X., Lao, K., & Xie, X. S. (2006) · Science · doi:10.1126/science.1119623 · Cited by 918
Cited by
Paving the way to single-molecule protein sequencing
Restrepo-Pérez, L., Joo, C., & Dekker, C. (2018) · Nature Nanotechnology · doi:10.1038/s41565-018-0236-6 · Cited by 396
Label-free biological and chemical sensors
Hunt, H. K., & Armani, A. M. (2010) · Nanoscale · doi:10.1039/c0nr00201a · Cited by 371
Single Biomolecule Imaging by Electrochemiluminescence
Liu, Y., Zhang, H., Li, B., Liu, J., Jiang, D., Liu, B., & Sojic, N. (2021) · Journal of the American Chemical Society · doi:10.1021/jacs.1c06673 · Cited by 303
Digital Quantification of Proteins and mRNA in Single Mammalian Cells
Albayrak, C., Jordi, C. A., Zechner, C., Lin, J., Bichsel, C. A., Khammash, M., & Tay, S. (2016) · Molecular Cell · doi:10.1016/j.molcel.2016.02.030 · Cited by 176
Han, L., Zi, X., Garmire, L. X., Wu, Y., Weissman, S. M., Pan, X., & Fan, R. (2014) · Scientific Reports · doi:10.1038/srep06485 · Cited by 83
Metadata
- Authors
- Huang, B., Wu, H., Bhaya, D., Grossman, A., Granier, S., Kobilka, B. K., & Zare, R. N.
- Venue
- Science
- Published
- 2007-01-05
- Volume
- 315
- Issue
- 5808
- Pages
- 81-84
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W1974511084
- Cited by
- 387
- References
- 3
- FWCI
- 30.17
- Type
- journal article
- Language
- en
- Open access
- closed
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