The DOI matches its Crossref record; 28 of the 28 references it cites were found in a registry or an index.
Probing Transcription Factor Dynamics at the Single-Molecule Level in a Living Cell
Open accessAbstract
Transcription factors regulate gene expression through their binding to DNA. In a living Escherichia coli cell, we directly observed specific binding of a lac repressor, labeled with a fluorescent protein, to a chromosomal lac operator. Using single-molecule detection techniques, we measured the kinetics of binding and dissociation of the repressor in response to metabolic signals. Furthermore, we characterized the nonspecific binding to DNA, one-dimensional (1D) diffusion along DNA segments, and 3D translocation among segments through cytoplasm at the single-molecule level. In searching for the operator, a lac repressor spends ∼90% of time nonspecifically bound to and diffusing along DNA with a residence time of <5 milliseconds. The methods and findings can be generalized to other nucleic acid binding proteins.
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Growth Rate-Dependent Global Effects on Gene Expression in Bacteria
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CTCF and cohesin regulate chromatin loop stability with distinct dynamics
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Single-Molecule Dynamics of Enhanceosome Assembly in Embryonic Stem Cells
Chen, J., Zhang, Z., Li, L., Chen, B.-C., Revyakin, A., Hajj, B., Legant, W., Dahan, M., Lionnet, T., Betzig, E., et al. (2014) · Cell · doi:10.1016/j.cell.2014.01.062 · Cited by 602
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Metadata
- Authors
- Elf, J., Li, G.-W., & Xie, X. S.
- Venue
- Science
- Published
- 2007-05-25
- Volume
- 316
- Issue
- 5828
- Pages
- 1191-1194
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2110823478
- Cited by
- 1,084
- References
- 28
- FWCI
- 25.98
- Type
- journal article
- Language
- en
- Open access
- green
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