Cited by 680Genomics and Chromatin DynamicsGene Regulatory Network AnalysisRNA Research and Splicing

Real-Time Observation of Transcription Initiation and Elongation on an Endogenous Yeast Gene

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Larson, Daniel R., Zenklusen, Daniel, Wu, Bin, Chao, Jeffrey A., Singer, Robert H.·2011·Science

Abstract

In yeast, the initiation of gene expression is stochastic and is controlled by transcription factor search times.

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Crossref · 10.1126/science.1202142

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  • Other imaging techniques reported RNA Pol II speed between 0.6 and 12 kb/min.

    “Other imaging techniques such as fluctuation analysis of fluorescently labeled nascent RNA or high‐speed 3D fluorescence nanoimaging techniques reported speed in the same range, albeit slightly higher between 0.6 and 12 kb/min (Larson et al, 2011; Martin et al, 2013; Annibale & Gratton, 2015).”

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Research works printed but not linked: 1

  1. [13]Elson E Magde D Biopolymers 13 1 1974 4818131

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Authors
Larson, D. R., Zenklusen, D., Wu, B., Chao, J. A., & Singer, R. H.
Affiliations
Albert Einstein College of Medicine (US)
Corresponding author
Singer, Robert H.
Venue
Science
Published
2011-04-22
Volume
332
Issue
6028
Pages
475-478
ISSN
  • 0036-8075 (ISSN-L)
  • 1095-9203
OpenAlex
W2017886758
Cited by
680
References
30
Citations by year
2026: 31 · 2025: 44 · 2024: 57 · 2023: 32 · 2022: 52 · 2021: 54 · 2020: 45 · 2019: 51 · 2018: 37 · 2017: 46 · 2016: 42 · 2015: 40 · 2014: 37 · 2013: 43 · 2012: 40
FWCI
19.07
Type
journal article
Language
en
Open access
Locations
  • Science (publishedVersion)
  • PubMed (publishedVersion)
  • Europe PMC (PubMed Central) (acceptedVersion)
  • PubMed Central (acceptedVersion)
Funders
National Institute of General Medical Sciences (57071, 86217)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gene Regulatory Network Analysis
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
Keywords
transcription initiation, transcription elongation, nascent RNA, fluctuation analysis, Saccharomyces cerevisiae, transcriptional memory
MeSH terms
ATPases Associated with Diverse Cellular Activities, Adenosine Triphosphatases, Cell Cycle, Cell Nucleus, DNA Polymerase I, Genes, Fungal, Glutamate Synthase, Kinetics, Microscopy, Fluorescence, Models, Genetic, Promoter Regions, Genetic, RNA Polymerase II, RNA Precursors, RNA, Fungal, RNA, Messenger, Saccharomyces cerevisiae, Spectrometry, Fluorescence, Transcription Factors, Transcription, Genetic, Transcriptional Activation, Saccharomyces cerevisiae Proteins, Green Fluorescent Proteins, Facilitated Diffusion

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