Real-Time Observation of Transcription Initiation and Elongation on an Endogenous Yeast Gene
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In yeast, the initiation of gene expression is stochastic and is controlled by transcription factor search times.
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Other imaging techniques reported RNA Pol II speed between 0.6 and 12 kb/min.
“Other imaging techniques such as fluctuation analysis of fluorescently labeled nascent RNA or high‐speed 3D fluorescence nanoimaging techniques reported speed in the same range, albeit slightly higher between 0.6 and 12 kb/min (Larson et al, 2011; Martin et al, 2013; Annibale & Gratton, 2015).”
From RNA polymerase II speed: a key player in controlling and adapting transcriptome composition (2021) · cites this paper as Larson et al, 2011
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- Authors
- Larson, D. R., Zenklusen, D., Wu, B., Chao, J. A., & Singer, R. H.
- Affiliations
- Albert Einstein College of Medicine (US)
- Corresponding author
- Singer, Robert H.
- Venue
- Science
- Published
- 2011-04-22
- Volume
- 332
- Issue
- 6028
- Pages
- 475-478
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- PMC
- PMC3152976
- OpenAlex
- W2017886758
- Cited by
- 680
- References
- 30
- Citations by year
- 2026: 31 · 2025: 44 · 2024: 57 · 2023: 32 · 2022: 52 · 2021: 54 · 2020: 45 · 2019: 51 · 2018: 37 · 2017: 46 · 2016: 42 · 2015: 40 · 2014: 37 · 2013: 43 · 2012: 40
- FWCI
- 19.07
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Science (publishedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- Funders
- National Institute of General Medical Sciences (57071, 86217)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gene Regulatory Network Analysis
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
- Keywords
- transcription initiation, transcription elongation, nascent RNA, fluctuation analysis, Saccharomyces cerevisiae, transcriptional memory
- MeSH terms
- ATPases Associated with Diverse Cellular Activities, Adenosine Triphosphatases, Cell Cycle, Cell Nucleus, DNA Polymerase I, Genes, Fungal, Glutamate Synthase, Kinetics, Microscopy, Fluorescence, Models, Genetic, Promoter Regions, Genetic, RNA Polymerase II, RNA Precursors, RNA, Fungal, RNA, Messenger, Saccharomyces cerevisiae, Spectrometry, Fluorescence, Transcription Factors, Transcription, Genetic, Transcriptional Activation, Saccharomyces cerevisiae Proteins, Green Fluorescent Proteins, Facilitated Diffusion
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