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Cited by 645Chromosomal and Genetic VariationsRNA Interference and Gene DeliveryPlant Molecular Biology Research

Endogenous siRNAs Derived from Transposons and mRNAs in Drosophila Somatic Cells

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Ghildiyal, Megha, Seitz, Hervé, Horwich, Michael D., Li, Chengjian, Du, Tingting, Lee, Soohyun, Xu, Jia, Kittler, Ellen L.W. +3 more·2008·Science
DOI 10.1126/science.1157396PMC PMC2953241
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Abstract

Small interfering RNAs (siRNAs) direct RNA interference (RNAi) in eukaryotes. In flies, somatic cells produce siRNAs from exogenous double-stranded RNA (dsRNA) as a defense against viral infection. We identified endogenous siRNAs (endo-siRNAs), 21 nucleotides in length, that correspond to transposons and heterochromatic sequences in the somatic cells of Drosophila melanogaster. We also detected endo-siRNAs complementary to messenger RNAs (mRNAs); these siRNAs disproportionately mapped to the complementary regions of overlapping mRNAs predicted to form double-stranded RNA in vivo. Normal accumulation of somatic endo-siRNAs requires the siRNA-generating ribonuclease Dicer-2 and the RNAi effector protein Argonaute2 (Ago2). We propose that endo-siRNAs generated by the fly RNAi pathway silence selfish genetic elements in the soma, much as Piwi-interacting RNAs do in the germ line.

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  • piRNAs are known to exist in the Drosophila and mammalian brain.

    “While the function of piwi in regard to piRNA production and transposable element silencing is well established in the germline29, and piRNAs are known to exist in the Drosophila and mammalian brain30,31, it is currently unknown if piwi reduction affects transposable element transcripts in the brain.”

    From Pathogenic tau-induced piRNA depletion promotes neuronal death through transposable element dysregulation in neurodegenerative tauopathies (2018) · cites this paper as [30]

    The passage describing Fig. 4C (indexed as Abstract; page unavailable) reports piRNA-like RNAs in Drosophila ago2-mutant heads. It does not establish that these are piRNAs in the brain, and the paper provides no evidence for piRNAs in mammalian brains. Its findings therefore support only a narrower part of the claim. (LLM, conf=0.82)

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References 26 · Cited by 645

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  1. [1]Gunawardane LS, et al. Science. 2007;315:1587. doi: 10.1126/science.1140494. published online 21 February 2007 (10.1126/science.1140494) [DOI] [PubMed] [Google Scholar] · DOI
  2. [2]Brennecke J, et al. Cell. 2007;128:1089. doi: 10.1016/j.cell.2007.01.043. [DOI] [PubMed] [Google Scholar] · DOI · Paper page
  3. [3]Vagin VV, et al. Science. 2006;313:320. doi: 10.1126/science.1129333. published online 28 June 2006 (10.1126/science.1129333) [DOI] [PubMed] [Google Scholar] · DOI
  4. [4]Yin H, Lin H. Nature. 2007;450:304. doi: 10.1038/nature06263. [DOI] [PubMed] [Google Scholar] · DOI
  5. [5]Hamilton A, Voinnet O, Chappell L, Baulcombe D. EMBO J. 2002;21:4671. doi: 10.1093/emboj/cdf464. [DOI] [PMC free article] [PubMed] [Google Scholar] · DOI
  6. [6]Sunkar R, Girke T, Zhu JK. Nucleic Acids Res. 2005;33:4443. doi: 10.1093/nar/gki758. [DOI] [PMC free article] [PubMed] [Google Scholar] · DOI
  7. [7]Yang N, Kazazian HHJ. Nat. Struct. Mol. Biol. 2006;13:763. doi: 10.1038/nsmb1141. [DOI] [PubMed] [Google Scholar] · DOI
  8. [8]Ishizuka A, Siomi MC, Siomi H. Genes Dev. 2002;16:2497. doi: 10.1101/gad.1022002. [DOI] [PMC free article] [PubMed] [Google Scholar] · DOI · Paper page
  9. [9]Csink AK, Linsk R, Birchler JA. Genetics. 1994;138:153. doi: 10.1093/genetics/138.1.153. [DOI] [PMC free article] [PubMed] [Google Scholar] · DOI
  10. [10]Rehwinkel J, et al. Mol. Cell. Biol. 2006;26:2965. doi: 10.1128/MCB.26.8.2965-2975.2006. [DOI] [PMC free article] [PubMed] [Google Scholar] · DOI
  11. [11]Haynes KA, Caudy AA, Collins L, Elgin SC. Curr. Biol. 2006;16:2222. doi: 10.1016/j.cub.2006.09.035. [DOI] [PMC free article] [PubMed] [Google Scholar] · DOI
  12. [12]Pal-Bhadra M, Bhadra U, Birchler JA. Mol. Cell. 2002;9:315. doi: 10.1016/s1097-2765(02)00440-9. [DOI] [PubMed] [Google Scholar] · DOI
  13. [13]Tchurikov NA, Kretova OV. PLoS ONE. 2007;2:e476. doi: 10.1371/journal.pone.0000476. [DOI] [PMC free article] [PubMed] [Google Scholar] · DOI
  14. [14]Horwich MD, et al. Curr. Biol. 2007;17:1265. doi: 10.1016/j.cub.2007.06.030. [DOI] [PubMed] [Google Scholar] · DOI
  15. [15]Pelisson A, Sarot E, Payen-Groschene G, Bucheton A. J. Virol. 2007;81:1951. doi: 10.1128/JVI.01980-06. [DOI] [PMC free article] [PubMed] [Google Scholar] · DOI
  16. [16]Saito K, et al. Genes Dev. 2007;21:1603. doi: 10.1101/gad.1563607. [DOI] [PMC free article] [PubMed] [Google Scholar] · DOI
  17. [17]Okamura K, Ishizuka A, Siomi H, Siomi MC. Genes Dev. 2004;18:1655. doi: 10.1101/gad.1210204. [DOI] [PMC free article] [PubMed] [Google Scholar] · DOI
  18. [18]Nykanen A, Haley B, Zamore PD. Cell. 2001;107:309. doi: 10.1016/s0092-8674(01)00547-5. [DOI] [PubMed] [Google Scholar] · DOI
  19. [20]Lee YS, Carthew RW. Methods. 2003;30:322. doi: 10.1016/s1046-2023(03)00051-3. [DOI] [PubMed] [Google Scholar] · DOI
  20. [21]Seitz H, Ghildiyal M, Zamore PD. Curr. Biol. 2008;18:147. doi: 10.1016/j.cub.2007.12.049. [DOI] [PMC free article] [PubMed] [Google Scholar] · DOI · Paper page
  21. [22]Zamore PD, Tuschl T, Sharp PA, Bartel DP. Cell. 2000;101:25. doi: 10.1016/S0092-8674(00)80620-0. [DOI] [PubMed] [Google Scholar] · DOI
  22. [23]Desset S, Buchon N, Meignin C, Coiffet M, Vaury C. PLoS ONE. 2008;3:e1526. doi: 10.1371/journal.pone.0001526. [DOI] [PMC free article] [PubMed] [Google Scholar] · DOI
  23. [25]Czech B, et al. Nature. 2008 10.1038/nature07007. [Google Scholar] · DOI
  24. [26]Kawamura Y, et al. Nature. 2008 10.1038/nature06938. [Google Scholar] · DOI
  25. [27]Okamura K, et al. Nature. 2008 10.1038/nature07015. [Google Scholar] · DOI

Authors
Ghildiyal, M., Seitz, H., Horwich, M. D., Li, C., Du, T., Lee, S., Xu, J., Kittler, E. L. W., Zapp, M. L., Weng, Z., & Zamore, P. D.
Affiliations
  • Boston University (US)
  • Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01605, USA.
Corresponding author
  • Ghildiyal, Megha
  • Zamore, Phillip D.
ORCID
Venue
Science
Publisher
American Association for the Advancement of Science (AAAS)
Published
2008-05-23
Volume
320
Issue
5879
Pages
1077-1081
ISSN
  • 0036-8075 (ISSN-L)
  • 1095-9203
DOI
10.1126/science.1157396
PMC
PMC2953241
OpenAlex
W1991007428
Cited by
645
References
26
Citations by year
2026: 8 · 2025: 19 · 2024: 15 · 2023: 17 · 2022: 23 · 2021: 26 · 2020: 24 · 2019: 25 · 2018: 34 · 2017: 31 · 2016: 26 · 2015: 36 · 2014: 45 · 2013: 62 · 2012: 52
FWCI
81.24
Type
journal article
Language
en
Open access
Locations
  • Science (publishedVersion)
  • PubMed (publishedVersion)
  • PubMed Central (acceptedVersion)
  • eScholarship@UMassChan (submittedVersion)
Funders
  • Howard Hughes Medical Institute
  • National Institutes of Health (5r01hg003367-03)
  • National Institute on Aging (F30AG030283)
  • National Human Genome Research Institute (R01 HG003367, HG003367)
  • National Institute of General Medical Sciences (R01GM062862, GM080625, R01GM080625, GM62862, R01GM065236, GM65236, R37GM062862)
  • National Institute of Allergy and Infectious Diseases (P30AI042845, R01AI043208)
Topics
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Chromosomal and Genetic Variations
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Interference and Gene Delivery
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Molecular Biology Research
Keywords
endogenous small interfering RNAs, RNA interference, transposable elements, heterochromatin, Dicer-2, Argonaute 2, selfish genetic elements, piRNA, gene silencing
MeSH terms
Animals, Base Sequence, Cell Line, DNA Transposable Elements, Drosophila melanogaster, Mutation, RNA, Double-Stranded, RNA, Messenger, Retroelements, RNA Helicases, Drosophila Proteins, RNA Interference, RNA, Small Interfering, RNA-Induced Silencing Complex, Ribonuclease III, Argonaute Proteins

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