The DOI matches its Crossref record; 22 of the 27 references it cites were found in a registry or an index.
Direct Restart of a Replication Fork Stalled by a Head-On RNA Polymerase
Open accessAbstract
When Polymerases Collide: Genomes must be replicated by DNA polymerase and copied by RNA polymerase. The two processes occur concurrently in bacteria and, although the majority of bacterial genes are co-oriented with replication, head-on collisions still occur and can result in double-strand breaks in the genome. Pomerantz and O'Donnell (p. 590 ) show, in vitro, that both Escherichia coli DNA and RNA polymerases grind to a halt when they collide bumper to bumper but that the replication fork remains intact, with the RNA polymerase being shunted off the DNA and out of the way. Mfd, a transcription-repair coupling factor, promotes DNA replication restart probably by rewinding separated DNA stands ahead of the halted transcription complex, promoting its displacement from the DNA.
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Citations
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Metadata
- Authors
- Pomerantz, R. T., & O’Donnell, M.
- Affiliations
- Howard Hughes Medical Institute (US)
- Corresponding author
- O’Donnell, Mike
- Venue
- Science
- Published
- 2010-01-29
- Volume
- 327
- Issue
- 5965
- Pages
- 590-592
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- PMC
- PMC2861996
- OpenAlex
- W2086307439
- Cited by
- 150
- References
- 27
- Citations by year
- 2026: 3 · 2025: 7 · 2024: 4 · 2023: 8 · 2022: 7 · 2021: 10 · 2020: 10 · 2019: 4 · 2018: 4 · 2017: 6 · 2016: 15 · 2015: 9 · 2014: 7 · 2013: 17 · 2012: 14
- FWCI
- 7.30
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Science (publishedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- Funders
- Howard Hughes Medical Institute
- National Institute of General Medical Sciences (R01GM038839)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA Repair Mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Keywords
- replication fork restart, replication fork stalling, replisome, transcription-coupled repair, Mfd, Escherichia coli, DNA replication, transcription-replication conflict
- MeSH terms
- Bacterial Proteins, DNA-Directed DNA Polymerase, DNA Repair, DNA Replication, DNA, Bacterial, Escherichia coli, DNA-Directed RNA Polymerases, Templates, Genetic, Transcription Factors, Transcription, Genetic, Escherichia coli Proteins
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