The DOI matches its Crossref record; 6 of the 6 references it cites were found in a registry or an index.
Single β-Actin mRNA Detection in Neurons Reveals a Mechanism for Regulating Its Translatability
Open accessAbstract
The physical manifestation of learning and memory formation in the brain can be expressed by strengthening or weakening of synaptic connections through morphological changes. Local actin remodeling underlies some forms of plasticity and may be facilitated by local β-actin synthesis, but dynamic information is lacking. In this work, we use single-molecule in situ hybridization to demonstrate that dendritic β-actin messenger RNA (mRNA) and ribosomes are in a masked, neuron-specific form. Chemically induced long-term potentiation prompts transient mRNA unmasking, which depends on factors active during synaptic activity. Ribosomes and single β-actin mRNA motility increase after stimulation, indicative of release from complexes. Hence, the single-molecule assays we developed allow for the quantification of activity-induced unmasking and availability for active translation. Further, our work demonstrates that β-actin mRNA and ribosomes are in a masked state that is alleviated by stimulation.
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Cites
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FMRP Stalls Ribosomal Translocation on mRNAs Linked to Synaptic Function and Autism
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Characterization of an RNA Granule from Developing Brain
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Cited by
Spatially resolved, highly multiplexed RNA profiling in single cells
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Single-Cell Phenotyping within Transparent Intact Tissue through Whole-Body Clearing
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Dynamic Axonal Translation in Developing and Mature Visual Circuits
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Metadata
- Authors
- Buxbaum, A. R., Wu, B., & Singer, R. H.
- Venue
- Science
- Published
- 2014-01-24
- Volume
- 343
- Issue
- 6169
- Pages
- 419-422
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2082832087
- Cited by
- 280
- References
- 6
- FWCI
- 14.49
- Type
- journal article
- Language
- en
- Open access
- green
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