The DOI matches its Crossref record; 70 of the 70 references it cites were found in a registry or an index.
Lattice light-sheet microscopy: Imaging molecules to embryos at high spatiotemporal resolution
Open accessAbstract
From single molecules to embryos in living color: Animation defines life, and the three-dimensional (3D) imaging of dynamic biological processes occurring within living specimens is essential to understand life. However, in vivo imaging, especially in 3D, involves inevitable tradeoffs of resolution, speed, and phototoxicity. Chen et al. describe a microscope that can address these concerns. They used a class of nondiffracting beams, known as 2D optical lattices, which spread the excitation energy across the entire field of view while simultaneously eliminating out-of-focus excitation. Lattice light sheets increase the speed of image acquisition and reduce phototoxicity, which expands the range of biological problems that can be investigated. The authors illustrate the power of their approach using 20 distinct biological systems ranging from single-molecule binding kinetics to cell migration and division, immunology, and embryonic development. Science , this issue 10.1126/science.1257998
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Mediator and RNA polymerase II clusters associate in transcription-dependent condensates
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Applying systems-level spectral imaging and analysis to reveal the organelle interactome
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Metadata
- Authors
- Chen, B.-C., Legant, W. R., Wang, K., Shao, L., Milkie, D. E., Davidson, M. W., Janetopoulos, C., Wu, X. S., Hammer, J. A., Liu, Z., English, B. P., Mimori-Kiyosue, Y., Romero, D. P., Ritter, A. T., Lippincott-Schwartz, J., Fritz-Laylin, L., Mullins, R. D., Mitchell, D. M., Bembenek, J. N., Reymann, A.-C., Böhme, R., Grill, S. W., Wang, J. T., Seydoux, G., Tulu, U. S., Kiehart, D. P., & Betzig, E.
- Venue
- Science
- Published
- 2014-10-24
- Volume
- 346
- Issue
- 6208
- Pages
- 1257998-1257998
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2053270078
- Cited by
- 1,919
- References
- 70
- FWCI
- 77.86
- Type
- journal article
- Language
- en
- Open access
- green
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- Chen, B.-C., Legant, W. R., Wang, K., Shao, L., Milkie, D. E., Davidson, M. W., Janetopoulos, C., Wu, X. S., Hammer, J. A., Liu, Z., English, B. P., Mimori-Kiyosue, Y., Romero, D. P., Ritter, A. T., Lippincott-Schwartz, J., Fritz-Laylin, L., Mullins, R. D., Mitchell, D. M., Bembenek, J. N., . . . Betzig, E. (2014). Lattice light-sheet microscopy: Imaging molecules to embryos at high spatiotemporal resolution. Science. https://doi.org/10.1126/science.1257998
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