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Imaging Intracellular Fluorescent Proteins at Nanometer Resolution
Abstract
We introduce a method for optically imaging intracellular proteins at nanometer spatial resolution. Numerous sparse subsets of photoactivatable fluorescent protein molecules were activated, localized (to ∼2 to 25 nanometers), and then bleached. The aggregate position information from all subsets was then assembled into a superresolution image. We used this method—termed photoactivated localization microscopy—to image specific target proteins in thin sections of lysosomes and mitochondria; in fixed whole cells, we imaged vinculin at focal adhesions, actin within a lamellipodium, and the distribution of the retroviral protein Gag at the plasma membrane.
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Cited by
Welsh, J. A., Goberdhan, D. C. I., O'Driscoll, L., Buzas, E. I., Blenkiron, C., Bussolati, B., Cai, H., Di Vizio, D., Driedonks, T. A. P., Erdbrügger, U., et al. (2024) · Journal of Extracellular Vesicles · doi:10.1002/jev2.12404 · Cited by 4,663
Hell, S. W. (2007) · Science · doi:10.1126/science.1137395 · Cited by 2,967
Lattice light-sheet microscopy: Imaging molecules to embryos at high spatiotemporal resolution
Chen, B.-C., Legant, W. R., Wang, K., Shao, L., Milkie, D. E., Davidson, M. W., Janetopoulos, C., Wu, X. S., Hammer, J. A., Liu, Z., et al. (2014) · Science · doi:10.1126/science.1257998 · Cited by 1,919
Self-citationTranscriptome-scale super-resolved imaging in tissues by RNA seqFISH
Eng, C.-H. L., Lawson, M., Zhu, Q., Dries, R., Koulena, N., Takei, Y., Yun, J., Cronin, C., Karp, C., Yuan, G.-C., et al. (2019) · Nature · doi:10.1038/s41586-019-1049-y · Cited by 1,754
Super-resolution fluorescence microscopy
Huang, B., Bates, M., & Zhuang, X. (2009) · Annual Review of Biochemistry · doi:10.1146/annurev.biochem.77.061906.092014 · Cited by 1,657
Metadata
- Authors
- Betzig, E., Patterson, G. H., Sougrat, R., Lindwasser, O. W., Olenych, S., Bonifacino, J. S., Davidson, M. W., Lippincott-Schwartz, J., & Hess, H. F.
- Venue
- Science
- Published
- 2006-09-15
- Volume
- 313
- Issue
- 5793
- Pages
- 1642-1645
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2054455698
- Cited by
- 8,772
- References
- 0
- FWCI
- 100.38
- Type
- journal article
- Language
- en
- Open access
- closed
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- Betzig, E., Patterson, G. H., Sougrat, R., Lindwasser, O. W., Olenych, S., Bonifacino, J. S., Davidson, M. W., Lippincott-Schwartz, J., & Hess, H. F. (2006). Imaging Intracellular Fluorescent Proteins at Nanometer Resolution. Science. https://doi.org/10.1126/science.1127344
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