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Polymerase II Promoter Activation: Closed Complex Formation and ATP-Driven Start Site Opening
Abstract
Studies on bacterial RNA polymerases have divided the initiation pathway into three steps, namely (i) promoter binding to form the closed complex; (ii) DNA melting to form an open complex, and (iii) messenger RNA initiation. Potassium permanganate was used to detect DNA melting by mammalian RNA polymerase II in vitro. Closed complexes formed in a rate-limiting step that was stimulated by the activator GAL4-VP16. Adenosine triphosphate was then hydrolyzed to rapidly melt the DNA within the closed complex to form an open complex. Addition of nucleoside triphosphates resulted in the melted bubble moving away from the start site, completing initiation.
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Cited by
Wada, T., Takagi, T., Yamaguchi, Y., Ferdous, A., Imai, T., Hirose, S., Sugimoto, S., Yano, K., Hartzog, G. A., Winston, F., et al. (1998) · Genes & Development · doi:10.1101/gad.12.3.343 · Cited by 750
Synergism in transcriptional activation: a kinetic view.
Herschlag, D., & Johnson, F. B. (1993) · Genes & Development · doi:10.1101/gad.7.2.173 · Cited by 190
Molecular Structure of Human TFIIH
Schultz, P., Fribourg, S., Poterszman, A., Mallouh, V., Moras, D., & Egly, J. M. (2000) · Cell · doi:10.1016/s0092-8674(00)00082-9 · Cited by 180
Pause & go: from the discovery of RNA polymerase pausing to its functional implications
Mayer, A., Landry, H. M., & Churchman, L. S. (2017) · Current Opinion in Cell Biology · doi:10.1016/j.ceb.2017.03.002 · Cited by 125
Structural basis of SNAPc-dependent snRNA transcription initiation by RNA polymerase II
(2022) · doi:10.1038/s41594-022-00857-w · Cited by 19
Metadata
- Authors
- Wang, W., Carey, M., & Gralla, J. D.
- Venue
- Science
- Published
- 1992-01-24
- Volume
- 255
- Issue
- 5043
- Pages
- 450-453
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2090554343
- Cited by
- 218
- References
- 0
- FWCI
- 10.31
- Type
- journal article
- Language
- en
- Open access
- closed
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- Wang, W., Carey, M., & Gralla, J. D. (1992). Polymerase II Promoter Activation: Closed Complex Formation and ATP-Driven Start Site Opening. Science. https://doi.org/10.1126/science.1310361
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
