The DOI matches its Crossref record; 23 of the 23 references it cites were found in a registry or an index.
Activation of Mitogen-Activated Protein Kinase Kinase by v-Raf in NIH 3T3 Cells and in Vitro
Abstract
Mitogen-activated protein (MAP) kinases are 42- and 44-kD serine-threonine protein kinases that are activated by tyrosine and threonine phosphorylation in cells stimulated with mitogens and growth factors. MAP kinase and the protein kinase that activates it (MAP kinase kinase) were constitutively activated in NIH 3T3 cells infected with viruses containing either of two oncogenic forms (p35 EC12 , p37 22W ) of the c-Raf-1 protein kinase. The v-Raf proteins purified from cells infected with EC12 or 22W viruses activated MAP kinase kinase from skeletal muscle in vitro. Furthermore, a bacterially expressed v-Raf fusion protein (glutathione S-transferase-p37 22W ) also activated MAP kinase kinase in vitro. These findings suggest that one function of c-Raf-1 in mitogenic signaling is to phosphorylate and activate MAP kinase kinase.
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Cites
Okadaic acid: a new probe for the study of cellular regulation
Cohen, P., Holmes, C. F. B., & Tsukitani, Y. (1990) · Trends in Biochemical Sciences · doi:10.1016/0968-0004(90)90192-e · Cited by 1,387
Raf-1 activates MAP kinase-kinase
Kyriakis, J. M., App, H., Zhang, X.-F., Banerjee, P., Brautigan, D. L., Rapp, U. R., & Avruch, J. (1992) · Nature · doi:10.1038/358417a0 · Cited by 1,283
Payne, D. M., Rossomando, A. J., Martino, P., Erickson, A. K., Her, J. H., Shabanowitz, J., Hunt, D. F., Weber, M. J., & Sturgill, T. W. (1991) · The EMBO Journal · doi:10.1002/j.1460-2075.1991.tb08021.x · Cited by 1,040
Self-citationWood, K. W., Sarnecki, C., Roberts, T. M., & Blenis, J. (1992) · Cell · doi:10.1016/0092-8674(92)90076-o · Cited by 903
Self-citationThomas, S. M., DeMarco, M., D'Arcangelo, G., Halegoua, S., & Brugge, J. S. (1992) · Cell · doi:10.1016/0092-8674(92)90075-n · Cited by 676
Ray, L. B., & Sturgill, T. W. (1987) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.84.6.1502 · Cited by 592
Self-citationDissection of the protein kinase cascade by which nerve growth factor activates MAP kinases
Gómez, N., & Cohen, P. (1991) · Nature · doi:10.1038/353170a0 · Cited by 569
Raf-1 protein kinase is required for growth of induced NIH/3T3 cells
Kolch, W., Heidecker, G., Lloyd, P., & Rapp, U. R. (1991) · Nature · doi:10.1038/349426a0 · Cited by 474
Mitogen-activated protein kinases: versatile transducers for cell signaling
Pelech, S. L., & Sanghera, J. S. (1992) · Trends in Biochemical Sciences · doi:10.1016/s0968-0004(00)80005-5 · Cited by 428
Involvement of p21ras in activation of extracellular signal-regulated kinase 2
de Vries-Smits, A. M. M., Burgering, B. M. T., Leevers, S. J., Mar shall, C. J., & Bos, J. L. (1992) · Nature · doi:10.1038/357602a0 · Cited by 387
Cited by
Mitogen-Activated Protein Kinases: Specific Messages from Ubiquitous Messengers
Schaeffer, H. J., & Weber, M. J. (1999) · Molecular and Cellular Biology · doi:10.1128/mcb.19.4.2435 · Cited by 1,600
A Divergence in the MAP Kinase Regulatory Network Defined by MEK Kinase and Raf
Lange-Carter, C. A., Pleiman, C. M., Gardner, A. M., Blumer, K. J., & Johnson, G. L. (1993) · Science · doi:10.1126/science.8385802 · Cited by 827
Activation mechanism of the MAP kinase ERK2 by dual phosphorylation
Canagarajah, B. J., Khokhlatchev, A., Cobb, M. H., & Goldsmith, E. J. (1997) · Cell · doi:10.1016/s0092-8674(00)80351-7 · Cited by 729
Ras history: The saga continues
Cox, A. D., & Der, C. J. (2010) · Small GTPases · doi:10.4161/sgtp.1.1.12178 · Cited by 706
Raf meets Ras: completing the framework of a signal transduction pathway
Avruch, J., Zhang, X.-F., & Kyriakis, J. M. (1994) · Trends in Biochemical Sciences · doi:10.1016/0968-0004(94)90005-1 · Cited by 577
Metadata
- Authors
- Dent, P., Haser, W., Haystead, T. A. J., Vincent, L. A., Roberts, T. M., & Sturgill, T. W.
- Venue
- Science
- Published
- 1992-09-04
- Volume
- 257
- Issue
- 5075
- Pages
- 1404-1407
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2009338295
- Cited by
- 668
- References
- 23
- FWCI
- 40.59
- Type
- journal article
- Language
- en
- Open access
- closed
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- Dent, P., Haser, W., Haystead, T. A. J., Vincent, L. A., Roberts, T. M., & Sturgill, T. W. (1992). Activation of Mitogen-Activated Protein Kinase Kinase by v-Raf in NIH 3T3 Cells and in Vitro. Science. https://doi.org/10.1126/science.1326789
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