Arthropod molting hormone: Radioimmune assay
Abstract
A radioimmune assay for the arthropod molting hormone, ecdysterone, has been developed. The sensitivity of the assay is 200 picograms or 25 times the maximum sensitivity of the bioassay. Closely related steroids also bind the anti-body, but with lower affinities.
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Borst and O'Connor developed the first immunoassay for ecdysteroids.
“Borst and O'Connor [145] developed the first immunoassay for ecdysteroids, and, over the subsequent years, several others have been developed [146][147][148][149][150][151][152][153][154][155][156][157][158][159].”
From Ecdysteroids (2021) · cites this paper as [145]
Cites
CONTROL OF MOLTING IN MANDIBULATE AND CHELICERATE ARTHROPODS BY ECDYSONES
Krishnakumaran, A., & Schneiderman, H. A. (1970) · Biological Bulletin · doi:10.2307/1540371 · Cited by 106 · see citing works
Assay for ecdysone (Molting hormone) activity using the house fly, Musca domestica L.
Kaplanis, J. N., Tabor, L. A., Thompson, M. J., Robbins, W. E., & Shortino, T. J. (1966) · Steroids · doi:10.1016/0039-128x(66)90003-1 · Cited by 94 · see citing works
Gas Chromatographic Separation of Phytoecdysones
IKEKAWA, N., Hattori, F., Rubio-Lightbourn, J., et al. (1972) · Journal of Chromatographic Science · doi:10.1093/chromsci/10.4.233 · Cited by 50 · see citing works
Eine verbesserte, sehr empfindliche Methode zur biologischen Auswertung des Insektenhormones Ecdyson
Adelung, D., & Karlson, P. (1969) · Journal of Insect Physiology · doi:10.1016/0022-1910(69)90192-9 · Cited by 38 · see citing works
AN IMPROVED CALLIPHORA BIOASSAY FOR INSECT MOULTING HORMONES
Thomson, J., Imray, F. P., & Hohn, D. (1970) · Immunology and Cell Biology · doi:10.1038/icb.1970.34 · Cited by 23 · see citing works
An apparatus for investigation of heterogeneous reactions in a flow system
Albeck, M., & Rav‐Acha, C. (1970) · Cellular and Molecular Life Sciences · doi:10.1007/bf02114185 · Cited by 5 · see citing works
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Jones, C. G., & Firn, R. D. (1978) · Journal of Chemical Ecology · doi:10.1007/bf00988049 · Cited by 66 · see citing works
Lafont, R., Balducci, C., & Dinan, L. (2021) · Encyclopedia · doi:10.3390/encyclopedia1040096 · Cited by 49 · see citing works
- Authors
- Borst, D. W., & O'Connor, J. D.
- Affiliations
- University of California, Los Angeles (US)
- Department of Biology, University of California at Los Angeles, Los Angeles 90024
- Venue
- Science
- Published
- 1972-10-27
- Volume
- 178
- Issue
- 4059
- Pages
- 418-419
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2058933189
- Cited by
- 193
- References
- 6
- Citations by year
- 2023: 1 · 2022: 1 · 2021: 1 · 2017: 2 · 2016: 1 · 2014: 2 · 2013: 1 · 2012: 1
- FWCI
- 2.85
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Science (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neurobiology and Insect Physiology Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Steroid Chemistry and Biochemistry
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuropeptides and Animal Physiology
- Keywords
- 20-hydroxyecdysone, radioimmunoassay, steroid quantification, antibody affinity
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