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Messenger RNA Splicing in Yeast: Clues to Why the Spliceosome Is a Ribonucleoprotein
Abstract
The removal of introns from eukaryotic messenger RNA precursors shares mechanistic characteristics with the self-splicing of certain introns, prompting speculation that the catalytic reactions of nuclear pre-messenger RNA splicing are fundamentally RNA-based. The participation of five small nuclear RNAs (snRNAs) in splicing is now well documented. Genetic analysis in yeast has revealed the requirement, in addition, for several dozen proteins. Some of these are tightly bound to snRNAs to form small nuclear ribonucleoproteins (snRNPs); such proteins may promote interactions between snRNAs or between an snRNA and the intron. Other, non-snRNP proteins appear to associate transiently with the spliceosome. Some of these factors, which include RNA-dependent adenosine triphosphatases, may promote the accurate recognition of introns.
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Cited by
The C. elegans heterochronic gene lin-4 encodes small RNAs with antisense complementarity to lin-14
Lee, R. C., Feinbaum, R. L., & Ambros, V. (1993) · Cell · doi:10.1016/0092-8674(93)90529-y · Cited by 12,948
A general two-metal-ion mechanism for catalytic RNA
Steitz, T. A., & Steitz, J. A. (1993) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.90.14.6498 · Cited by 1,171
Sharp, P. (1994) · Cell · doi:10.1016/0092-8674(94)90130-9 · Cited by 567
Site-Specific Modification of Pre-mRNA: the 2′-Hydroxyl Groups at the Splice Sites
Moore, M. J., & Sharp, P. A. (1992) · Science · doi:10.1126/science.1589782 · Cited by 469
U5 snRNA interacts with exon sequences at 5′ and 3′ splice sites
Newman, A. J., & Norman, C. (1992) · Cell · doi:10.1016/0092-8674(92)90149-7 · Cited by 360
Metadata
- Authors
- Guthrie, C.
- Venue
- Science
- Published
- 1991-07-12
- Volume
- 253
- Issue
- 5016
- Pages
- 157-163
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W1988271866
- Cited by
- 418
- References
- 0
- FWCI
- 28.67
- Type
- journal article
- Language
- en
- Open access
- closed
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- Guthrie, C. (1991). Messenger RNA Splicing in Yeast: Clues to Why the Spliceosome Is a Ribonucleoprotein. Science. https://doi.org/10.1126/science.1853200
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