The DOI matches its Crossref record; 43 of the 43 references it cites were found in a registry or an index.
Site-Specific Modification of Pre-mRNA: the 2′-Hydroxyl Groups at the Splice Sites
Abstract
A simple and efficient method for synthesizing long, site-specifically modified RNA molecules was developed whereby segments of RNA were joined with the use of bacteriophage T4 DNA ligase. A single hydrogen or O -methyl group was substituted for the 2′-hydroxyl group at either splice site of a nuclear pre-messenger RNA substrate. Splicing of the modified pre-messenger RNA's in vitro revealed that, although a 2′-hydroxyl is not absolutely required at either splice site, the 2′-hydroxyl at the 3′ splice site is important for the second step of splicing. These results are compared to previous studies of analogous 2′-hydroxyl groups in the self-splicing Tetrahymena group I intron.
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Cites
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Saenger, W. (1984) · Springer Advanced Texts in Chemistry · doi:10.1007/978-1-4612-5190-3 · Cited by 4,150
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Splicing of Messenger RNA Precursors
Sharp, P. A. (1987) · Science · doi:10.1126/science.3544217 · Cited by 901
Self-citationRNA evolution and the origins of life
Joyce, G. F. (1989) · Nature · doi:10.1038/338217a0 · Cited by 658
The role of small nuclear ribonucleoprotein particles in pre-mRNA splicing
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The Chemistry of Self-Splicing RNA and RNA Enzymes
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On the origin of RNA splicing and introns
Sharp, P. A. (1985) · Cell · doi:10.1016/0092-8674(85)90092-3 · Cited by 485
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Cited by
RNA-catalyzed RNA polymerization: Accurate and general RNA-templated primer extension.
Johnston, W. K., Unrau, P. J., Lawrence, M. S., Glasner, M. E., & Bartel, D. P. (2001) · Science · doi:10.1126/science.1060786 · Cited by 768
Initiation of Protein Synthesis by the Eukaryotic Translational Apparatus on Circular RNAs
Chen, C.-Y., & Sarnow, P. (1995) · Science · doi:10.1126/science.7536344 · Cited by 766
Sharp, P. (1994) · Cell · doi:10.1016/0092-8674(94)90130-9 · Cited by 567
Self-citationSensing Self and Foreign Circular RNAs by Intron Identity
Chen, Y. G., Kim, M. V., Chen, X., Batista, P. J., Aoyama, S., Wilusz, J. E., Iwasaki, A., & Chang, H. Y. (2017) · Molecular Cell · doi:10.1016/j.molcel.2017.05.022 · Cited by 475
RNA circularization strategies in vivo and in vitro
Petkovic, S., & Müller, S. (2015) · Nucleic Acids Research · doi:10.1093/nar/gkv045 · Cited by 386
“T4 DNA ligase-mediated RNA ligation was pioneered by Moore and Sharp (78,79), and ever since has been used in a number of protocols.”
Background
Metadata
- Authors
- Moore, M. J., & Sharp, P. A.
- Venue
- Science
- Published
- 1992-05-15
- Volume
- 256
- Issue
- 5059
- Pages
- 992-997
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2006144062
- Cited by
- 469
- References
- 43
- FWCI
- 9.67
- Type
- journal article
- Language
- en
- Open access
- closed
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
