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Cloning and Expression of a Rat Brain GABA Transporter
Abstract
A complementary DNA clone (designated GAT-1) encoding a transporter for the neurotransmitter γ-aminobutyric acid (GABA) has been isolated from rat brain, and its functional properties have been examined in Xenopus oocytes. Oocytes injected with GAT-1 synthetic messenger RNA accumulated [ 3 H]GABA to levels above control values. The transporter encoded by GAT-1 has a high affinity for GABA, is sodium-and chloride-dependent, and is pharmacologically similar to neuronal GABA transporters. The GAT-1 protein shares antigenic determinants with a native rat brain GABA transporter. The nucleotide sequence of GAT-1 predicts a protein of 599 amino acids with a molecular weight of 67 kilodaltons. Hydropathy analysis of the deduced protein suggests multiple transmembrane regions, a feature shared by several cloned transporters; however, database searches indicate that GAT-1 is not homologous to any previously identified proteins. Therefore, GAT-1 appears to be a member of a previously uncharacterized family of transport molecules.
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Cites
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
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Cited by
Danbolt, N. C. (2001) · Progress in Neurobiology · doi:10.1016/s0301-0082(00)00067-8 · Cited by 4,227
Bak, L. K., Schousboe, A., & Waagepetersen, H. S. (2006) · Journal of Neurochemistry · doi:10.1111/j.1471-4159.2006.03913.x · Cited by 1,114
Plasma membrane monoamine transporters: structure, regulation and function
Torres, G. E., Gainetdinov, R. R., & Caron, M. G. (2003) · Nature Reviews Neuroscience · doi:10.1038/nrn1008 · Cited by 939
Expression cloning of a cocaine-and antidepressant-sensitive human noradrenaline transporter
Pacholczyk, T., Blakely, R. D., & Amara, S. G. (1991) · Nature · doi:10.1038/350350a0 · Cited by 933
Local and diffuse synaptic actions of GABA in the hippocampus
Isaacson, J. S., Solis, J. M., & Nicoll, R. A. (1993) · Neuron · doi:10.1016/0896-6273(93)90308-e · Cited by 688
Metadata
- Authors
- Guastella, J., Nelson, N., Nelson, H., Czyzyk, L., Keynan, S., Miedel, M. C., Davidson, N., Lester, H. A., & Kanner, B. I.
- Venue
- Science
- Published
- 1990-09-14
- Volume
- 249
- Issue
- 4974
- Pages
- 1303-1306
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2022200746
- Cited by
- 879
- References
- 2
- FWCI
- 19.43
- Type
- journal article
- Language
- en
- Open access
- closed
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- Guastella, J., Nelson, N., Nelson, H., Czyzyk, L., Keynan, S., Miedel, M. C., Davidson, N., Lester, H. A., & Kanner, B. I. (1990). Cloning and Expression of a Rat Brain GABA Transporter. Science. https://doi.org/10.1126/science.1975955
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