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Protein Splicing Converts the Yeast TFP1 Gene Product to the 69-kdDSubunit of the Vacuolar H + -Adenosine Triphosphatase
Abstract
The TFP1 gene of the yeast Saccharomyces cerevisiae encodes two proteins: the 69-kilodalton (kD) catalytic subunit of the vacuolar proton-translocating adenosine triphosphatase (H + -ATPase) and a 50-kD protein. The 69-kD subunit is encoded by the 5′ and 3′ thirds of the TFP1 coding region, whereas the 50-kD protein is encoded by the central third. Evidence is presented that both the 69-kD and 50-kD proteins are obtained from a single translation product that is cleaved to release the 50-kD protein and spliced to form the 69-kD subunit.
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Citations
Cited by
Inteins: nature's gift to protein chemists
Shah, N. H., & Muir, T. W. (2014) · Chem. Sci. · doi:10.1039/c3sc52951g · Cited by 404
Xie, H., Vucetic, S., Iakoucheva, L. M., Oldfield, C. J., Dunker, A. K., Obradovic, Z., & Uversky, V. N. (2007) · Journal of Proteome Research · doi:10.1021/pr060394e · Cited by 382
Horizontal transfer of ATPase genes — the tree of life becomes a net of life
Hilario, E., & Gogarten, J. P. (1993) · Biosystems · doi:10.1016/0303-2647(93)90038-e · Cited by 241
Nature's recipe for splitting inteins
Aranko, A. S., Wlodawer, A., & Iwai, H. (2014) · Protein Engineering Design and Selection · doi:10.1093/protein/gzu028 · Cited by 116
Marrone, L., Marchi, P. M., & Azzouz, M. (2022) · Expert Opinion on Biological Therapy · doi:10.1080/14712598.2022.2012148 · Cited by 56
“nized the field of protein engineering earning the label of ‘nature’s gift to protein chemists’ [57], were discovered in 1990 within the genome of Saccharomyces cerevisiae [58,59],”
Background
Metadata
- Authors
- Kane, P. M., Yamashiro, C. T., Wolczyk, D. F., Neff, N., Goebl, M., & Stevens, T. H.
- Venue
- Science
- Published
- 1990-11-02
- Volume
- 250
- Issue
- 4981
- Pages
- 651-657
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W1977120851
- Cited by
- 497
- References
- 0
- FWCI
- 8.10
- Type
- journal article
- Language
- en
- Open access
- closed
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- CoChat. (2026). Citation check: Protein Splicing Converts the Yeast TFP1 Gene Product to the 69-kdDSubunit of the Vacuolar H + -Adenosine Triphosphatase. https://app.cochat.ai/papers/10.1126/science.2146742
- The paper
- Kane, P. M., Yamashiro, C. T., Wolczyk, D. F., Neff, N., Goebl, M., & Stevens, T. H. (1990). Protein Splicing Converts the Yeast TFP1 Gene Product to the 69-kdDSubunit of the Vacuolar H + -Adenosine Triphosphatase. Science. https://doi.org/10.1126/science.2146742
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
