Cited by 345HIV Research and TreatmentHIV/AIDS drug development and treatmentVirus-based gene therapy research

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Retroviral DNA Integration Directed by HIV Integration Protein in Vitro

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Bushman, Frederic D., Fujiwara, Tamio, Craigie, Robert·1990·Science

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Abstract

Efficient retroviral growth requires integration of a DNA copy of the viral RNA genome into a chromosome of the host. As a first step in analyzing the mechanism of integration of human immunodeficiency virus (HIV) DNA, a cell-free system was established that models the integration reaction. The in vitro system depends on the HIV integration (IN) protein, which was partially purified from insect cells engineered to express IN protein in large quantities. Integration was detected in a biological assay that scores the insertion of a linear DNA containing HIV terminal sequences into a λ DNA target. Some integration products generated in this assay contained five-base pair duplications of the target DNA at the recombination junctions, a characteristic of HIV integration in vivo; the remaining products contained aberrant junctional sequences that may have been produced in a variation of the normal reaction. These results indicate that HIV IN protein is the only viral protein required to insert model HIV DNA sequences into a target DNA in vitro.

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Crossref · 10.1126/science.2171144

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DNA sequencing with chain-terminating inhibitors
Sanger, F., Nicklen, S., & Coulson, A. R. (1977) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.74.12.5463 · Cited by 68,572
Sequence from picomole quantities of proteins electroblotted onto polyvinylidene difluoride membranes.
Matsudaira, P. T. (1987) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)61070-1 · Cited by 4,951
Production of acquired immunodeficiency syndrome-associated retrovirus in human and nonhuman cells transfected with an infectious molecular clone
Adachi, A., Gendelman, H. E., Koenig, S., Folks, T. M., Willey, R. L., Rabson, A. B., & Martín, M. Á. (1986) · Journal of Virology · doi:10.1128/jvi.59.2.284-291.1986 · Cited by 2,805
Nucleic acid structure and expression of the human AIDS/lymphadenopathy retrovirus
Muesing, M. A., Smith, D. H., Cabradilla, C. D., Benton, C. V., Lasky, L. A., & Capon, D. J. (1985) · Nature · doi:10.1038/313450a0 · Cited by 684
Computer analysis of retroviral pol genes: assignment of enzymatic functions to specific sequences and homologies with nonviral enzymes
Johnson, M. S., McClure, M. A., Feng, D. F., Gray, J., & Doolittle, R. F. (1986) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.83.20.7648 · Cited by 480
Correct integration of retroviral DNA in vitro
Brown, P. O., Bowerman, B. A., VARMUS, H. E., & Bishop, J. M. (1987) · Cell · doi:10.1016/0092-8674(87)90287-x · Cited by 460
The IN protein of Moloney murine leukemia virus processes the viral DNA ends and accomplishes their integration in vitro
Craigie, R., Fujiwara, T., & Bushman, F. (1990) · Cell · doi:10.1016/0092-8674(90)90126-y · Cited by 409
Self-citation
Retroviral integration: structure of the initial covalent product and its precursor, and a role for the viral IN protein
Brown, P. O., Bowerman, B., Varmus, H. E., & Bishop, J. M. (1989) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.86.8.2525 · Cited by 399
Retroviral DNA integration: structure of an integration intermediate
Fujiwara, T., & Mizuuchi, K. (1988) · Cell · doi:10.1016/0092-8674(88)90071-2 · Cited by 385
Self-citation
A nucleoprotein complex mediates the integration of retroviral DNA.Preprint
Bowerman, B. A., Brown, P. O., Bishop, J. M., & Varmus, H. E. (1989) · Genes & Development · doi:10.1101/gad.3.4.469 · Cited by 378

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Show all 26 references

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Donehower, L. A., Proceedings of the National Academy of Sciences of the United States of America 81: 6461 (1984).
Panganiban, A. T., Proceedings of the National Academy of Sciences of the United States of America 81: 7885 (1984).
VARMUS, H, MOBILE DNA: 53 (1989).

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Pemberton, I. K., Buckle, M., & BUC, H. C. (1996) · Journal of Biological Chemistry · doi:10.1074/jbc.271.3.1498 · Cited by 62
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Triplex-mediated inhibition of HIV DNA integration in vitro.
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Self-citation
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Li, L., Farnet, C. M., Anderson, W. F., & Bushman, F. D. (1998) · Journal of Virology · doi:10.1128/jvi.72.3.2125-2131.1998 · Cited by 49
Self-citation
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Pahl, A., & Flügel, R. M. (1993) · Journal of Virology · doi:10.1128/jvi.67.9.5426-5434.1993 · Cited by 48
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Self-citation
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Integration complexes derived from HIV vectors for rapid assays in vitro
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Self-citation
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A covalent complex between retroviral integrase and nicked substrate DNA.
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Self-citation
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Self-citation
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Self-citation
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Nilsen, B. M., Haugan, I. R., Berg, K., Olsen, L., Brown, P. O., & Helland, D. E. (1996) · Journal of Virology · doi:10.1128/jvi.70.3.1580-1587.1996 · Cited by 44
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Self-citation
Retroviral integration: in vitro host site selection by avian integrase
Fitzgerald, M. L., & Grandgenett, D. P. (1994) · Journal of Virology · doi:10.1128/jvi.68.7.4314-4321.1994 · Cited by 42
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Hassounah, S. A., Alikhani, A., Oliveira, M., Bharaj, S., Ibanescu, R.-I., Osman, N., Xu, H.-T., Brenner, B. G., Mesplède, T., & Wainberg, M. A. (2017) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.01695-17 · Cited by 40
Multimodal mechanism of action of allosteric HIV-1 integrase inhibitors
Jurado, K. A., & Engelman, A. (2013) · Expert Reviews in Molecular Medicine · doi:10.1017/erm.2013.15 · Cited by 43
The HIV-1 Integrase Monomer Induces a Specific Interaction with LTR DNA for Concerted Integration
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Therapeutic modulation of gene expression in the disease state: Treatment strategies and approaches for the development of next-generation of the epigenetic drugs
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Design, synthesis and biological evaluation of heteroaryl diketohexenoic and diketobutanoic acids as HIV-1 integrase inhibitors endowed with antiretroviral activity
Santo, R. D., Costi, R., Artico, M., Ragno, R., Greco, G., Novellino, E., Marchand, C., & Pommier, Y. G. (2005) · Il Farmaco · doi:10.1016/j.farmac.2005.03.008 · Cited by 39
Ty3 integrase mutants defective in reverse transcription or 3'-end processing of extrachromosomal Ty3 DNA
Kirchner, J., & Sandmeyer, S. B. (1996) · Journal of Virology · doi:10.1128/jvi.70.7.4737-4747.1996 · Cited by 39
Mutations affecting interaction of integrase with TNPO3 do not prevent HIV-1 cDNA nuclear import
Cribier, A., Ségéral, E., Delelis, O., Parissi, V., Simon, A., Ruff, M., Bénarous, R., & Emiliani, S. (2011) · Retrovirology · doi:10.1186/1742-4690-8-104 · Cited by 48
3'-end processing and kinetics of 5'-end joining during retroviral integration in vivo
Roe, T., Chow, S. A., & Brown, P. O. (1997) · Journal of Virology · doi:10.1128/jvi.71.2.1334-1340.1997 · Cited by 38
Retrotransposition mechanisms
Boeke, J. D., & Chapman, K. B. (1991) · Current Opinion in Cell Biology · doi:10.1016/0955-0674(91)90079-e · Cited by 37
Characterization of Mg2+-Dependent 3'-Processing Activity for Human Immunodeficiency Virus Type 1 Integrase in Vitro: Real-Time Kinetic Studies Using Fluorescence Resonance Energy Transfer
Lee, S. P., Kim, H. G., Censullo, M. L., & Han, M. K. (1995) · Biochemistry · doi:10.1021/bi00032a014 · Cited by 37
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Characterization of recombinant murine leukemia virus integrase
Dotan, I., Scottoline, B. P., Heuer, T. S., & Brown, P. O. (1995) · Journal of Virology · doi:10.1128/jvi.69.1.456-468.1995 · Cited by 36
Avian Retrovirus U3 and U5 DNA Inverted Repeats
Vora, A. C., Chiu, R., McCord, M., Goodarzi, G., Stahl, S. J., Mueser, T. C., Hyde, C. C., & Grandgenett, D. P. (1997) · Journal of Biological Chemistry · doi:10.1074/jbc.272.38.23938 · Cited by 36
Proteomic Analysis of Early HIV-1 Nucleoprotein Complexes
Schweitzer, C. J., Jagadish, T., Haverland, N. A., Ciborowski, P. S., & Belshan, M. (2013) · Journal of Proteome Research · doi:10.1021/pr300869h · Cited by 35
Poly(ADP-Ribose) Polymerase 1 Is Not Strictly Required for Infection of Murine Cells by Retroviruses
Siva, A., & Bushman, F. (2002) · Journal of Virology · doi:10.1128/jvi.76.23.11904-11910.2002 · Cited by 36
Self-citation
Multimodal Functionalities of HIV-1 Integrase
Engelman, A., & Kvaratskhelia, M. (2022) · Viruses · doi:10.3390/v14050926 · Cited by 37
Cellular Cofactors of Lentiviral Integrase: From Target Validation to Drug Discovery
Taltynov, O., Desimmie, B. A., Demeulemeester, J., Christ, F., & Debyser, Z. (2012) · Molecular Biology International · doi:10.1155/2012/863405 · Cited by 35
Viral gene products and replication of the human immunodeficiency type 1 virus
Morrow, C. D., Park, J., & Wakefield, J. (1994) · American Journal of Physiology-Cell Physiology · doi:10.1152/ajpcell.1994.266.5.c1135 · Cited by 34
HIV type 1 integrase inhibitors: from basic research to clinical implications.
Jegede, O., Babu, J., Santo, R. D., McColl, D. J., Weber, J., & Quiñones‐Mateu, M. E. (2008) · PubMed · Cited by 32
Folding of the multidomain human immunodeficiency virus type‐I integrase
Grandgenett, D. P., & Goodarzi, G. (1994) · Protein Science · doi:10.1002/pro.5560030604 · Cited by 33
Retroviral cDNA Integration: Mechanism, Applications and Inhibition
Hansen, M. S. T., Carteau, S., Hoffmann, C. J., Li, L., & Bushman, F. D. (1998) · Springer eBooks · doi:10.1007/978-1-4899-1739-3_3 · Cited by 33
Self-citation
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Self-citation

Authors: Bushman, F. D., Fujiwara, T., & Craigie, R.
Affiliations: National Institutes of Health (US)
ORCID: Bushman, Frederic D.
Craigie, Robert
Venue: Science
Publisher: American Association for the Advancement of Science (AAAS)
Published: 1990-09-28
Volume: 249
Issue: 4976
Pages: 1555-1558
ISSN: 0036-8075 (ISSN-L)
1095-9203
DOI: 10.1126/science.2171144
OpenAlex: W2050569727
Cited by: 338
References: 26
Citations by year: 2026: 1 · 2025: 1 · 2023: 3 · 2022: 3 · 2021: 7 · 2020: 1 · 2019: 5 · 2018: 2 · 2017: 5 · 2016: 7 · 2015: 11 · 2014: 12 · 2013: 8 · 2012: 9
FWCI: 6.48
Type: journal article
Language: en
Open access: green
zenodo.org
Licence: public-domain
Locations: Science (publishedVersion)
PubMed (publishedVersion)
Zenodo (CERN European Organization for Nuclear Research) (submittedVersion, public-domain)
Topics: Life Sciences › Immunology and Microbiology › Virology › HIV Research and Treatment
Health Sciences › Medicine › Infectious Diseases › HIV/AIDS drug development and treatment
Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Virus-based gene therapy research
Keywords: retroviral integration, HIV-1, terminal repeats, recombination junctions, site-specific integration
MeSH terms: Animals, Base Sequence, Cell Line, Cell-Free System, DNA Transposable Elements, DNA Nucleotidyltransferases, DNA, Viral, HIV, Insect Viruses, Models, Structural, Molecular Sequence Data, Leukemia Virus, Murine, Nucleic Acid Conformation, Restriction Mapping, Oligonucleotide Probes, Integrases

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Retroviral DNA Integration Directed by HIV Integration Protein in Vitro

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