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A Simple and General Method for Transferring Genes into Plants
Abstract
Transformed petunia, tobacco, and tomato plants have been produced by means of a novel leaf disk transformation-regeneration method. Surface-sterilized leaf disks were inoculated with an Agrobacterium tumefaciens strain containing a modified tumor-inducing plasmid (in which the phytohormone biosynthetic genes from transferred DNA had been deleted and replaced with a chimeric gene for kanamycin resistance) and cultured for 2 days. The leaf disks were then transferred to selective medium containing kanamycin. Shoot regeneration occurred within 2 to 4 weeks, and transformants were confirmed by their ability to form roots in medium containing kanamycin. This method for producing transformed plants combines gene transfer, plant regeneration, and effective selection for transformants into a single process and should be applicable to plant species that can be infected by Agrobacterium and regenerated from leaf explants.
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Cited by
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Werner, T., Motyka, V., Strnad, M., & Schmülling, T. (2001) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.171304098 · Cited by 1,133
Sirikantaramas, S., Taura, F., Tanaka, Y., Ishikawa, Y., Morimoto, S., & Shoyama, Y. (2005) · Plant and Cell Physiology · doi:10.1093/pcp/pci166 · Cited by 237
Romano, C. P., Hein, M. B., & Klee, H. J. (1991) · Genes & Development · doi:10.1101/gad.5.3.438 · Cited by 232
Expression of an Expansin Gene Is Correlated with Root Elongation in Soybean
Lee, D.-K., Ahn, J. H., Song, S.-K., Choi, Y. D., & Lee, J. S. (2003) · Plant Physiology · doi:10.1104/pp.009902 · Cited by 187
Engineering of complex protein sialylation in plants
Kallolimath, S., Castilho, A., Strasser, R., Grünwald-Gruber, C., Altmann, F., Strubl, S., Galuska, C. E., Zlatina, K., Galuska, S. P., Werner, S., et al. (2016) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1604371113 · Cited by 113
Metadata
- Authors
- Horsch, R. B., Fry, J. E., Hoffmann, N. L., Wallroth, M., Eichholtz, D., Rogers, S. G., & Fraley, R. T.
- Venue
- Science
- Published
- 1985-03-08
- Volume
- 227
- Issue
- 4691
- Pages
- 1229-1231
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2109975826
- Cited by
- 4,694
- References
- 0
- FWCI
- 39.53
- Type
- journal article
- Language
- en
- Open access
- closed
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- Horsch, R. B., Fry, J. E., Hoffmann, N. L., Wallroth, M., Eichholtz, D., Rogers, S. G., & Fraley, R. T. (1985). A Simple and General Method for Transferring Genes into Plants. Science. https://doi.org/10.1126/science.227.4691.1229
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
