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Mouse Embryonic Stem Cells and Reporter Constructs to Detect Developmentally Regulated Genes
Abstract
A strategy was devised for identifying regions of the mouse genome that are transcriptionally active in a temporally and spatially restricted manner during development. The approach is based on the introduction into embryonic stem cells of two types of lacZ reporter constructs that can be activated by flanking mouse genomic sequences. Embryonic stem cells containing the lacZ constructs were used to produce chimaeric mice. Developmental regulation of lacZ expression occurred at a high frequency. Molecular cloning of the flanking endogenous genes and introduction of these potential insertional mutations into the mouse germ line should provide an efficient means of identifying and mutating novel genes important for the control of mammalian development.
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Citations
Cited by
Imaging Neuronal Subsets in Transgenic Mice Expressing Multiple Spectral Variants of GFP
Feng, G., Mellor, R. H., Bernstein, M., Keller-Peck, C., Nguyen, Q. T., Wallace, M., Nerbonne, J. M., Lichtman, J. W., & Sanes, J. R. (2000) · Neuron · doi:10.1016/s0896-6273(00)00084-2 · Cited by 3,042
A conditional knockout resource for the genome-wide study of mouse gene function
Skarnes, W. C., Rosen, B., West, A. P., Koutsourakis, M., Bushell, W., Iyer, V., Mujica, A. O., Thomas, M., Harrow, J., Cox, T., et al. (2011) · Nature · doi:10.1038/nature10163 · Cited by 1,708
Self-citationMuseum of spatial transcriptomics
Moses, L., & Pachter, L. (2022) · Nature Methods · doi:10.1038/s41592-022-01409-2 · Cited by 1,255
Friedrich, G., & Soriano, P. (1991) · Genes & Development · doi:10.1101/gad.5.9.1513 · Cited by 1,151
Multilineage differentiation from human embryonic stem cell lines
Odorico, J. S., Kaufman, D. S., & Thomson, J. A. (2001) · Stem Cells · doi:10.1634/stemcells.19-3-193 · Cited by 969
Metadata
- Authors
- Gossler, A., Joyner, A. L., Rossant, J., & Skarnes, W. C.
- Venue
- Science
- Published
- 1989-04-28
- Volume
- 244
- Issue
- 4903
- Pages
- 463-465
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2082103215
- Cited by
- 487
- References
- 0
- FWCI
- 11.41
- Type
- journal article
- Language
- en
- Open access
- closed
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- Gossler, A., Joyner, A. L., Rossant, J., & Skarnes, W. C. (1989). Mouse Embryonic Stem Cells and Reporter Constructs to Detect Developmentally Regulated Genes. Science. https://doi.org/10.1126/science.2497519
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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