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Generation of a Large Combinatorial Library of the Immunoglobulin Repertoire in Phage Lambda
Abstract
A novel bacteriophage lambda vector system was used to express in Escherichia coli a combinatorial library of Fab fragments of the mouse antibody repertoire. The system allows rapid and easy identification of monoclonal Fab fragments in a form suitable for genetic manipulation. It was possible to generate, in 2 weeks, large numbers of monoclonal Fab fragments against a transition state analog hapten. The methods described may supersede present-day hybridoma technology and facilitate the production of catalytic and other antibodies.
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Citations
Cited by
Drug Discovery: A Historical Perspective
Drews, J. (2000) · Science · doi:10.1126/science.287.5460.1960 · Cited by 2,760
Making antibodies by phage display technology
Winter, G., Griffiths, A. D., Hawkins, R. E., & Hoogenboom, H. R. (1994) · Annual Review of Immunology · doi:10.1146/annurev.iy.12.040194.002245 · Cited by 1,573
Hoogenboom, H. R., Griffiths, A. D., Johnson, K. S., Chiswell, D. J., Hudson, P., & Winter, G. (1991) · Nucleic Acids Research · doi:10.1093/nar/19.15.4133 · Cited by 1,020
At the Crossroads of Chemistry and Immunology: Catalytic Antibodies
Lerner, R. A., Benkovic, S. J., & Schultz, P. G. (1991) · Science · doi:10.1126/science.2024118 · Cited by 668
Self-citationAntibody arrays for high-throughput screening of antibody–antigen interactions
de Wildt, R. M. T., Mundy, C. R., Gorick, B. D., & Tomlinson, I. M. (2000) · Nature Biotechnology · doi:10.1038/79494 · Cited by 624
Metadata
- Authors
- Huse, W. D., Sastry, L., Iverson, S. A., Kang, A. S., Alting-Mees, M., Burton, D. R., Benkovic, S. J., & Lerner, R. A.
- Venue
- Science
- Published
- 1989-12-08
- Volume
- 246
- Issue
- 4935
- Pages
- 1275-1281
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2061530347
- Cited by
- 805
- References
- 0
- FWCI
- 72.54
- Type
- journal article
- Language
- en
- Open access
- closed
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- Huse, W. D., Sastry, L., Iverson, S. A., Kang, A. S., Alting-Mees, M., Burton, D. R., Benkovic, S. J., & Lerner, R. A. (1989). Generation of a Large Combinatorial Library of the Immunoglobulin Repertoire in Phage Lambda. Science. https://doi.org/10.1126/science.2531466
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
