The DOI matches its Crossref record.
Isolation of Single-Copy Human Genes from a Library of Yeast Artificial Chromosome Clones
Abstract
A recently developed cloning system based on the propagation of large DNA molecules as linear, artificial chromosomes in the yeast Saccharomyces cerevisiae provides a potential method of cloning the entire human genome in segments of several hundred kilobase pairs. Most applications of this system will require the ability to recover specific sequences from libraries of yeast artificial chromosome clones and to propagate these sequences in yeast without alterations. Two single-copy genes have now been cloned from a library of yeast artificial chromosome clones that was prepared from total human DNA. Multiple, independent isolates were obtained of the genes encoding factor IX and plasminogen activator inhibitor type 2. The clones, which ranged in size from 60 to 650 kilobases, were stable on prolonged propagation in yeast and appear to contain faithful replicas of human DNA.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cited by
Expansion of an unstable trinucleotide CAG repeat in spinocerebellar ataxia type 1
Orr, H. T., Chung, M.-Y., Banfi, S., Kwiatkowski, T. J., Servadio, A., Beaudet, A. L., McCall, A. E., Duvick, L. A., Ranum, L. P. W., & Zoghbi, H. Y. (1993) · Nature Genetics · doi:10.1038/ng0793-221 · Cited by 1,632
Botstein, D., & Risch, N. (2003) · Nature Genetics · doi:10.1038/ng1090 · Cited by 1,503
Green, E. D., & Olson, M. V. (1990) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.87.3.1213 · Cited by 451
Self-citationPentao, L., Wise, C. A., Chinault, A. C., Patel, P. I., & Lupski, J. R. (1992) · Nature Genetics · doi:10.1038/ng1292-292 · Cited by 386
Chesi, M., Bergsagel, P., Brents, L., Smith, C., Gerhard, D., & Kuehl, W. (1996) · Blood · doi:10.1182/blood.v88.2.674.bloodjournal882674 · Cited by 277
Metadata
- Authors
- Brownstein, B. H., Silverman, G. A., Little, R. D., Burke, D. T., Korsmeyer, S. J., Schlessinger, D., & Olson, M. V.
- Venue
- Science
- Published
- 1989-06-16
- Volume
- 244
- Issue
- 4910
- Pages
- 1348-1351
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2040804173
- Cited by
- 316
- References
- 0
- FWCI
- 16.61
- Type
- journal article
- Language
- en
- Open access
- closed
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Isolation of Single-Copy Human Genes from a Library of Yeast Artificial Chromosome Clones. https://app.cochat.ai/papers/10.1126/science.2544027
- The paper
- Brownstein, B. H., Silverman, G. A., Little, R. D., Burke, D. T., Korsmeyer, S. J., Schlessinger, D., & Olson, M. V. (1989). Isolation of Single-Copy Human Genes from a Library of Yeast Artificial Chromosome Clones. Science. https://doi.org/10.1126/science.2544027
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
