The DOI matches its Crossref record; 17 of the 17 references it cites were found in a registry or an index.
Systematic screening of yeast artificial-chromosome libraries by use of the polymerase chain reaction.
Open accessAbstract
We have developed an approach for screening ordered arrays of yeast artificial-chromosome (YAC) clones containing human DNA that is based on the polymerase chain reaction (PCR). This approach is designed to determine the locations of positive clones within a YAC library that is stored as individual clones in 96-well microtiter plates. The high sensitivity and specificity of the PCR allow the detection of target sequences in DNA prepared from pools of 1920 or more YAC clones. The PCR-based screening protocol is performed in two successive stages, which effectively limit the location of a positive clone to four microtiter plates (384 clones). Final localization of each positive clone is accomplished by conventional DNA.DNA hybridization using a single filter containing the YAC clones from the appropriate four microtiter plates. This PCR-based screening strategy has proven highly efficient, allowing the identification and isolation of numerous YAC clones containing specific human genes. The prospects of developing a strategy for screening YAC libraries based completely on PCR assays are discussed, as are the potential applications of this approach to the systematic analysis of the human genome.
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The reeler gene encodes a protein with an EGF–like motif expressed by pioneer neurons
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Metadata
- Authors
- Green, E. D., & Olson, M. V.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1990-02
- Volume
- 87
- Issue
- 3
- Pages
- 1213-1217
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W1990074059
- Cited by
- 451
- References
- 17
- FWCI
- 21.61
- Type
- journal article
- Language
- en
- Open access
- green
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