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Alterations of Single Molecule Fluorescence Lifetimes in Near-Field Optical Microscopy
Abstract
Fluorescence lifetimes of single Rhodamine 6G molecules on silica surfaces were measured with pulsed laser excitation, time-correlated single photon counting, and near-field scanning optical microscopy (NSOM). The fluorescence lifetime varies with the position of a molecule relative to a near-field probe. Qualitative features of lifetime decreases are consistent with molecular excited state quenching effects near metal surfaces. The technique of NSOM provides a means of altering the environment of a single fluorescent molecule and its decay kinetics in a repeatable fashion.
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Citations
Cited by
Probing Individual Molecules with Confocal Fluorescence Microscopy
Nie, S., Chiu, D. T., & Zare, R. N. (1994) · Science · doi:10.1126/science.7973650 · Cited by 549
Bout, D. A. V., Yip, W.-T., Hu, D., Fu, D.-K., Swager, T. M., & Barbara, P. F. (1997) · Science · doi:10.1126/science.277.5329.1074 · Cited by 521
Near-Field Scanning Optical Microscopy
Dunn, R. C. (1999) · Chemical Reviews · doi:10.1021/cr980130e · Cited by 508
Controlled non-classical photon emission from single conjugated polymer molecules
Hollars, C. W., Lane, S. M., & Huser, T. (2003) · Chemical Physics Letters · doi:10.1016/s0009-2614(03)00091-5 · Cited by 96
Metadata
- Authors
- Ambrose, W. P., Goodwin, P. M., Keller, R. A., & Martin, J. C.
- Venue
- Science
- Published
- 1994-07-15
- Volume
- 265
- Issue
- 5170
- Pages
- 364-367
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W1983312070
- Cited by
- 341
- References
- 0
- FWCI
- 34.38
- Type
- journal article
- Language
- en
- Open access
- closed
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- CoChat. (2026). Citation check: Alterations of Single Molecule Fluorescence Lifetimes in Near-Field Optical Microscopy. https://app.cochat.ai/papers/10.1126/science.265.5170.364
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- Ambrose, W. P., Goodwin, P. M., Keller, R. A., & Martin, J. C. (1994). Alterations of Single Molecule Fluorescence Lifetimes in Near-Field Optical Microscopy. Science. https://doi.org/10.1126/science.265.5170.364
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Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
