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Cited by 549

The DOI matches its Crossref record; 25 of the 25 references it cites were found in a registry or an index.

Probing Individual Molecules with Confocal Fluorescence Microscopy

Nie, Shuming, Chiu, Daniel T., Zare, Richard N.·1994·Science

Abstract

Confocal fluorescence microscopy coupled with a diffraction-limited laser beam and a high-efficiency detection system has been used to study the diffusive movement and emission process of individual fluorescent molecules in the liquid phase at room temperature. The high detection sensitivity achieved at fast data acquisition speeds (greater than 1 kilohertz) allows real-time observation of single-molecule fluorescence without statistical analysis. The results show fluorescence-cycle saturation at the single-molecule level and multiple recrossings of a single molecule into and out of the probe volume as well as the triplet state.

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Metadata

Authors
Nie, S., Chiu, D. T., & Zare, R. N.
Venue
Science
Published
1994-11-11
Volume
266
Issue
5187
Pages
1018-1021
ISSN
  • 0036-8075 (ISSN-L)
  • 1095-9203
OpenAlex
W2004188090
Cited by
549
References
25
FWCI
20.63
Type
journal article
Language
en
Open access
closed

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CoChat. (2026). Citation check: Probing Individual Molecules with Confocal Fluorescence Microscopy. https://app.cochat.ai/papers/10.1126/science.7973650
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Nie, S., Chiu, D. T., & Zare, R. N. (1994). Probing Individual Molecules with Confocal Fluorescence Microscopy. Science. https://doi.org/10.1126/science.7973650

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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.