Direct measurement of coupling between dendritic spines and shafts
Abstract
Characterization of the diffusional and electrotonic coupling of spines to the dendritic shaft is crucial to understanding neuronal integration and synaptic plasticity. Two-photon photobleaching and photorelease of fluorescein dextran were used to generate concentration gradients between spines and shafts in rat CA1 pyramidal neurons. Diffusional reequilibration was monitored with two-photon fluorescence imaging. The time course of reequilibration was exponential, with time constants in the range of 20 to 100 milliseconds, demonstrating chemical compartmentalization on such time scales. These values imply that electrical spine neck resistances are unlikely to exceed 150 megohms and more likely range from 4 to 50 megohms.
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Two-photon microscopy overcomes limitations of confocal microscopy through the use of a pulsed infrared light source that excites fluorophores by the combined energy of two photons arriving on the sample nearly simultaneously.
“Two-photon microscopy overcomes both of these limitations through the use of a pulsed infrared light source that excites fluorophores by the combined energy of two photons arriving on the sample nearly simultaneously (Denk et al., 1990; Svoboda et al., 1996).”
From Barriers in the brain: resolving dendritic spine morphology and compartmentalization (2014) · cites this paper as Svoboda et al., 1996
Early studies found that spines can compartmentalize cytoplasm as fluorescence recovery rates in dendritic spines are significantly lower than in the shaft.
“Using two-photon fluorescence recovery after photobleaching (FRAP) microscopy on hippocampal neurons filled with fluorescent dyes in cultured slices, early studies found that spines can indeed compartmentalize cytoplasm as fluorescence recovery rates in dendritic spines are significantly lower than in the shaft (Svoboda et al., 1996).”
From Barriers in the brain: resolving dendritic spine morphology and compartmentalization (2014) · cites this paper as Svoboda et al., 1996
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References 33 · Cited by 472
- Authors
- Svoboda, K., Tank, D. W., & Denk, W.
- Affiliations
- Alcatel-Lucent (France) (FR)
- Corresponding author
- Denk, Winfried
- Venue
- Science
- Published
- 1996-05-03
- Volume
- 272
- Issue
- 5262
- Pages
- 716-719
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2066002146
- Cited by
- 472
- References
- 33
- Citations by year
- 2026: 9 · 2025: 4 · 2024: 6 · 2023: 11 · 2022: 13 · 2021: 9 · 2020: 9 · 2019: 10 · 2018: 10 · 2017: 12 · 2016: 12 · 2015: 25 · 2014: 23 · 2013: 20 · 2012: 25
- FWCI
- 6.09
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Science (publishedVersion)
- PubMed (publishedVersion)
- Cold Spring Harbor Laboratory Institutional Repository (Cold Spring Harbor Laboratory) (acceptedVersion)
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Neuroscience › Cognitive Neuroscience › Neural dynamics and brain function
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Ion channel regulation and function
- Keywords
- dendritic spines, electrical coupling, dendritic shafts, CA1 pyramidal neurons, fluorescein dextran, concentration gradient, neural integration, synaptic plasticity
- MeSH terms
- Animals, Dendrites, Dextrans, Diffusion, Electric Conductivity, Fluoresceins, Fluorescence, Kinetics, Microscopy, Models, Neurological, Neuronal Plasticity, Electric Impedance, Pyramidal Cells, Rats, In Vitro Techniques
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