Cited by 787Bacterial Genetics and BiotechnologyEnzyme Structure and FunctionDrug Transport and Resistance Mechanisms

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Siderophore-Mediated Iron Transport: Crystal Structure of FhuA with Bound Lipopolysaccharide

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Ferguson, Andrew D., Hofmann, Eckhard, Coulton, James W., Diederichs, Kay, Welte, Wolfram·1998·Science

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Abstract

FhuA, the receptor for ferrichrome-iron in Escherichia coli, is a member of a family of integral outer membrane proteins, which, together with the energy-transducing protein TonB, mediate the active transport of ferric siderophores across the outer membrane of Gram-negative bacteria. The three-dimensional structure of FhuA is presented here in two conformations: with and without ferrichrome-iron at resolutions of 2.7 and 2.5 angstroms, respectively. FhuA is a beta barrel composed of 22 antiparallel beta strands. In contrast to the typical trimeric arrangement found in porins, FhuA is monomeric. Located within the beta barrel is a structurally distinct domain, the "cork," which mainly consists of a four-stranded beta sheet and four short alpha helices. A single lipopolysaccharide molecule is noncovalently associated with the membrane-embedded region of the protein. Upon binding of ferrichrome-iron, conformational changes are transduced to the periplasmic pocket of FhuA, signaling the ligand-loaded status of the receptor. Sequence homologies and mutagenesis data are used to propose a structural mechanism for TonB-dependent siderophore-mediated transport across the outer membrane.

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Authors: Ferguson, A. D., Hofmann, E., Coulton, J. W., Diederichs, K., & Welte, W.
Affiliations: University of Konstanz (DE)
Corresponding author: Welte, Wolfram
ORCID: Ferguson, Andrew D.
Hofmann, Eckhard
Diederichs, Kay
Welte, Wolfram
Venue: Science
Publisher: American Association for the Advancement of Science (AAAS)
Published: 1998-12-18
Volume: 282
Issue: 5397
Pages: 2215-2220
ISSN: 0036-8075 (ISSN-L)
1095-9203
DOI: 10.1126/science.282.5397.2215
OpenAlex: W2084608838
Cited by: 787
References: 45
Citations by year: 2026: 7 · 2025: 9 · 2024: 15 · 2023: 10 · 2022: 10 · 2021: 17 · 2020: 13 · 2019: 14 · 2018: 16 · 2017: 21 · 2016: 19 · 2015: 12 · 2014: 22 · 2013: 24 · 2012: 27
FWCI: 22.45
Type: journal article
Language: en
Open access: green
nbn-resolving.de
Licence: cc-by-nc-nd
Locations: Science (publishedVersion)
PubMed (publishedVersion)
KOPS (University of Konstanz) (submittedVersion, cc-by-nc-nd)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
Life Sciences › Biochemistry, Genetics and Molecular Biology › Structural Biology › Enzyme Structure and Function
Health Sciences › Medicine › Oncology › Drug Transport and Resistance Mechanisms
Keywords: FhuA, siderophore transport, TonB, outer membrane receptor, lipopolysaccharide binding, beta-barrel proteins, Escherichia coli, Gram-negative bacteria, iron acquisition, conformational change, TonB-dependent transport, protein crystal structure
MeSH terms: Bacterial Outer Membrane Proteins, Bacterial Proteins, Binding Sites, Biological Transport, Active, Cell Membrane, Diffusion, Escherichia coli, Ferric Compounds, Ferrichrome, Hydrogen Bonding, Ligands, Lipopolysaccharides, Membrane Proteins, Models, Molecular, Protein Conformation, Receptors, Virus, Signal Transduction, Protein Structure, Secondary, Crystallography, X-Ray, Escherichia coli Proteins

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Siderophore-Mediated Iron Transport: Crystal Structure of FhuA with Bound Lipopolysaccharide

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