The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.
Detecting and Measuring Cotranslational Protein Degradation in Vivo
Abstract
Nascent polypeptides emerging from the ribosome and not yet folded may at least transiently present degradation signals similar to those recognized by the ubiquitin system in misfolded proteins. The ubiquitin sandwich technique was used to detect and measure cotranslational protein degradation in living cells. More than 50 percent of nascent protein molecules bearing an amino-terminal degradation signal can be degraded cotranslationally, never reaching their mature size before their destruction by processive proteolysis. Thus, the folding of nascent proteins, including abnormal ones, may be in kinetic competition with pathways that target these proteins for degradation cotranslationally.
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Cites
A multiubiquitin chain is confined to specific lysine in a targeted short-lived protein
Chau, V., Tobias, J. W., Bachmair, A., Marriott, D., Ecker, D. J., Gonda, D. K., & Varshavsky, A. (1989) · Science · doi:10.1126/science.2538923 · Cited by 1,535
Self-citationThe N-end rule: functions, mysteries, uses
Varshavsky, A. (1996) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.93.22.12142 · Cited by 859
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Myocytes Die by Multiple Mechanisms in Failing Human Hearts
Kostin, S., Pool, L., Elsässer, A., Hein, S., Drexler, H. C. A., Arnon, E., Hayakawa, Y., Zimmermann, R., Bauer, E., Klövekorn, W.-P., et al. (2003) · Circulation Research · doi:10.1161/01.res.0000067471.95890.5c · Cited by 564
Quantitating Protein Synthesis, Degradation, and Endogenous Antigen Processing
Princiotta, M. F., Finzi, D., Qian, S.-B., Gibbs, J., Schuchmann, S., Buttgereit, F., Bennink, J. R., & Yewdell, J. W. (2003) · Immunity · doi:10.1016/s1074-7613(03)00051-7 · Cited by 521
Proteasomal degradation of tau protein
David, D. C., Layfield, R., Serpell, L., Narain, Y., Goedert, M., & Spillantini, M. G. (2002) · Journal of Neurochemistry · doi:10.1046/j.1471-4159.2002.01137.x · Cited by 327
Nguyen, K. T., Lee, C.-S., Mun, S.-H., Truong, N. T., Park, S. K., & Hwang, C.-S. (2019) · Journal of Biological Chemistry · doi:10.1074/jbc.ra118.005556 · Cited by 89
Glenn, K. A., Nelson, R. F., Wen, H. M., Mallinger, A. J., & Paulson, H. L. (2008) · Journal of Biological Chemistry · doi:10.1074/jbc.m709508200 · Cited by 86
Metadata
- Authors
- Turner, G. C., & Varshavsky, A.
- Venue
- Science
- Published
- 2000-09-22
- Volume
- 289
- Issue
- 5487
- Pages
- 2117-2120
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2071847586
- Cited by
- 246
- References
- 2
- FWCI
- 5.29
- Type
- journal article
- Language
- en
- Open access
- closed
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