The DOI matches its Crossref record; 19 of the 19 references it cites were found in a registry or an index.
Inhibition of Eukaryotic DNA Replication by Geminin Binding to Cdt1
Abstract
In all eukaryotic organisms, inappropriate firing of replication origins during the G 2 phase of the cell cycle is suppressed by cyclin-dependent kinases. Multicellular eukaryotes contain a second putative inhibitor of re-replication called geminin. Geminin is believed to block binding of the mini-chromosome maintenance (MCM) complex to origins of replication, but the mechanism of this inhibition is unclear. Here we show that geminin interacts tightly with Cdt1, a recently identified replication initiation factor necessary for MCM loading. The inhibition of DNA replication by geminin that is observed in cell-free DNA replication extracts is reversed by the addition of excess Cdt1. In the normal cell cycle, Cdt1 is present only in G 1 and S, whereas geminin is present in S and G 2 phases of the cell cycle. Together, these results suggest that geminin inhibits inappropriate origin firing by targeting Cdt1.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Geminin, an Inhibitor of DNA Replication, Is Degraded during Mitosis
McGarry, T. J., & Kirschner, M. W. (1998) · Cell · doi:10.1016/s0092-8674(00)81209-x · Cited by 897
Mammalian Cell Fusion : Studies on the Regulation of DNA Synthesis and Mitosis
RAO, P. N., & JOHNSON, R. T. (1970) · Nature · doi:10.1038/225159a0 · Cited by 760
Two steps in the assembly of complexes at yeast replication origins in vivo
Diffley, J. F. X., Cocker, J. H., Dowell, S. J., & Rowley, A. (1994) · Cell · doi:10.1016/0092-8674(94)90299-2 · Cited by 561
Cell cycle control of DNA replication
Stillman, B. (1996) · Science · doi:10.1126/science.274.5293.1659 · Cited by 507
The Cdt1 protein is required to license DNA for replication in fission yeast
Nishitani, H., Lygerou, Z., Nishimoto, T., & Nurse, P. (2000) · Nature · doi:10.1038/35007110 · Cited by 469
Phosphorylation of mammalian CDC6 by Cyclin A/CDK2 regulates its subcellular localization
Petersen, B. O. (1999) · The EMBO Journal · doi:10.1093/emboj/18.2.396 · Cited by 430
INITIATION OF DNA REPLICATION IN EUKARYOTIC CELLS
Dutta, A., & Bell, S. P. (1997) · Annual Review of Cell and Developmental Biology · doi:10.1146/annurev.cellbio.13.1.293 · Cited by 393
Self-citationHayles, J., Fisher, D., Woollard, A., & Nurse, P. (1994) · Cell · doi:10.1016/s0092-8674(94)90542-8 · Cited by 382
XCDT1 is required for the assembly of pre-replicative complexes in Xenopus laevis
Maiorano, D., Moreau, J., & Méchali, M. (2000) · Nature · doi:10.1038/35007104 · Cited by 356
Replication checkpoint requires phosphorylation of the phosphatase Cdc25 by Cds1 or Chk1
Zeng, Y., Forbes, K. C., Wu, Z., Moreno, S., Piwnica-Worms, H., & Enoch, T. (1998) · Nature · doi:10.1038/26766 · Cited by 344
Cited by
The anaphase promoting complex/cyclosome: a machine designed to destroy
Peters, J.-M. (2006) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm1988 · Cited by 1,315
Nasmyth, K. (2001) · Annual Review of Genetics · doi:10.1146/annurev.genet.35.102401.091334 · Cited by 805
Genome-wide association study of hematological and biochemical traits in a Japanese population
Kamatani, Y., Matsuda, K., Okada, Y., Kubo, M., Hosono, N., Daigo, Y., Nakamura, Y., & Kamatani, N. (2010) · Nature Genetics · doi:10.1038/ng.531 · Cited by 503
Regulation of Early Events in Chromosome Replication
Diffley, J. F. X. (2004) · Current Biology · doi:10.1016/j.cub.2004.09.019 · Cited by 386
Initiating DNA synthesis: from recruiting to activating the MCM complex
Lei, M., & Tye, B. K. (2001) · Journal of Cell Science · doi:10.1242/jcs.114.8.1447 · Cited by 365
Metadata
- Authors
- Wohlschlegel, J. A., Dwyer, B. T., Dhar, S. K., Cvetic, C., Walter, J. C., & Dutta, A.
- Venue
- Science
- Published
- 2000-12-22
- Volume
- 290
- Issue
- 5500
- Pages
- 2309-2312
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2029925140
- Cited by
- 699
- References
- 19
- FWCI
- 14.72
- Type
- journal article
- Language
- en
- Open access
- closed
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Inhibition of Eukaryotic DNA Replication by Geminin Binding to Cdt1. https://app.cochat.ai/papers/10.1126/science.290.5500.2309
- The paper
- Wohlschlegel, J. A., Dwyer, B. T., Dhar, S. K., Cvetic, C., Walter, J. C., & Dutta, A. (2000). Inhibition of Eukaryotic DNA Replication by Geminin Binding to Cdt1. Science. https://doi.org/10.1126/science.290.5500.2309
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
