The DOI matches its Crossref record; 34 of the 34 references it cites were found in a registry or an index.
An integrated cell‐free metabolic platform for protein production and synthetic biology
Open accessAbstract
Cell-free systems offer a unique platform for expanding the capabilities of natural biological systems for useful purposes, i.e. synthetic biology. They reduce complexity, remove structural barriers, and do not require the maintenance of cell viability. Cell-free systems, however, have been limited by their inability to co-activate multiple biochemical networks in a single integrated platform. Here, we report the assessment of biochemical reactions in an Escherichia coli cell-free platform designed to activate natural metabolism, the Cytomim system. We reveal that central catabolism, oxidative phosphorylation, and protein synthesis can be co-activated in a single reaction system. Never before have these complex systems been shown to be simultaneously activated without living cells. The Cytomim system therefore promises to provide the metabolic foundation for diverse ab initio cell-free synthetic biology projects. In addition, we describe an improved Cytomim system with enhanced protein synthesis yields (up to 1200 mg/l in 2 h) and lower costs to facilitate production of protein therapeutics and biochemicals that are difficult to make in vivo because of their toxicity, complexity, or unusual cofactor requirements.
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Cites
Cell-free translation reconstituted with purified components
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Towards synthesis of a minimal cell
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Self-citationA new class of uncoupling agents — Carbonyl cyanide phenylhydrazones
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Construction of an in vitro bistable circuit from synthetic transcriptional switches
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Cited by
Cell-free gene expression: an expanded repertoire of applications
Silverman, A. D., Karim, A. S., & Jewett, M. C. (2020) · Nature Reviews Genetics · doi:10.1038/s41576-019-0186-3 · Cited by 593
Self-citationCell-free synthetic biology: Thinking outside the cell
Hodgman, C. E., & Jewett, M. C. (2012) · Metabolic Engineering · doi:10.1016/j.ymben.2011.09.002 · Cited by 425
Self-citationSystems biology perspectives on minimal and simpler cells
Xavier, J. C., Patil, K. R., & Rocha, I. (2014) · Microbiology and Molecular Biology Reviews · doi:10.1128/mmbr.00050-13 · Cited by 58
Metadata
- Authors
- Jewett, M. C., Calhoun, K. A., Voloshin, A., Wuu, J. J., & Swartz, J. R.
- Venue
- Molecular Systems Biology
- Published
- 2008-01
- Volume
- 4
- Issue
- 1
- Pages
- 220-220
- ISSN
- 1744-4292 (ISSN-L)
- OpenAlex
- W2111497213
- Cited by
- 384
- References
- 34
- FWCI
- 5.09
- Type
- journal article
- Language
- en
- Open access
- gold
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- Jewett, M. C., Calhoun, K. A., Voloshin, A., Wuu, J. J., & Swartz, J. R. (2008). An integrated cell‐free metabolic platform for protein production and synthetic biology. Molecular Systems Biology. https://doi.org/10.1038/msb.2008.57
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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