The DOI matches its Crossref record; 60 of the 60 references it cites were found in a registry or an index.
Induction of Membrane Ruffling and Fluid-Phase Pinocytosis in Quiescent Fibroblasts by ras Proteins
Abstract
Expression of the ras oncogene is thought to be one of the contributing events in the initiation of certain types of human cancer. To determine the cellular activities that are directly triggered by ras proteins, the early consequences of microinjection of the human H- ras proteins into quiescent rat embryo fibroblasts were investigated. Within 30 minutes to 1 hour after injection, cells show a marked increase in surface ruffles and fluid-phase pinocytosis. The rapid enhancement of membrane ruffling and pinocytosis is induced by both the proto-oncogenic and the oncogenic forms of the H- ras protein. The effects produced by the oncogenic protein persist for more than 15 hours after injection, whereas the effects of the proto-oncogenic protein are short-lived, being restricted to a 3-hour interval after injection. The stimulatory effect of the ras oncogene protein on ruffling and pinocytosis is dependent on the amount of injected protein and is accompanied by an apparent stimulation of phospholipase A 2 activity. These rapid changes in cell membrane activities induced by ras proteins may represent primary events in the mechanism of action of ras proteins.
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“The ability to stimulate macropinocytosis, a regulated form of endocytosis, is a distinctive feature of KRAS∗ activation,13 and PDAC cells harboring KRAS∗ rely on increased levels of macropinocytosis for nutrient salvaging to sustain uncontrolled cell growth.14”
From Therapeutic targeting of syndecan-1 axis overcomes acquired resistance to KRAS-targeted therapy in gastrointestinal cancers (2025) · cites this paper as [13]
In the citing paper
“The autophagy-MHC-I relationship provides mechanistic insight to functional genomics studies in mice, which have also linked autophagy and immune evasion in PDAC228.”
From Pancreatic cancer: Advances and challenges (2023) · cites this paper as [228]
Citations
Cites
Inositol trisphosphate and diacylglycerol as second messengers
Berridge, M. J. (1984) · Biochemical Journal · doi:10.1042/bj2200345 · Cited by 3,506
G proteins and dual control of adenylate cyclase
Gilman, A. G. (1984) · Cell · doi:10.1016/0092-8674(84)90336-2 · Cited by 1,772
Mechanism of activation of a human oncogene
Tabin, C. J., Bradley, S. M., Bargmann, C. I., Weinberg, R. A., Papageorge, A. G., Scolnick, E. M., Dhar, R., Lowy, D. R., & Chang, E. H. (1982) · Nature · doi:10.1038/300143a0 · Cited by 1,393
Reddy, E. P., Reynolds, R. K., Santos, E., & Barbacid, M. (1982) · Nature · doi:10.1038/300149a0 · Cited by 1,391
Intracellular phospholipases A
van den BOSCH, H. (1980) · Biochimica et Biophysica Acta (BBA) - Biomembranes · doi:10.1016/0005-2736(80)90574-x · Cited by 982
Human EJ bladder carcinoma oncogene is homologue of Harvey sarcoma virus ras gene
Parada, L. F., Tabin, C. J., Shih, C., & Weinberg, R. A. (1982) · Nature · doi:10.1038/297474a0 · Cited by 947
Requirement for ras proto-oncogene function during serum-stimulated growth of NIH 3T3 cells
Mulcahy, L. S., Smith, M. R., & Stacey, D. W. (1985) · Nature · doi:10.1038/313241a0 · Cited by 851
Expression of Cellular Oncogenes in Human Malignancies
Slamon, D. J., deKernion, J. B., Verma, I. M., & Cline, M. J. (1984) · Science · doi:10.1126/science.6538699 · Cited by 810
Zarbl, H., Sukumar, S. V., Arthur, A. V., Martı́n-Zanca, D., & Barbacid, M. (1985) · Nature · doi:10.1038/315382a0 · Cited by 807
Der, C. J., Krontiris, T. G., & Cooper, G. M. (1982) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.79.11.3637 · Cited by 793
Flagged first, then by citations
Research works printed but not linked: 2
- ALLISON, A.C., SYMPOSIA OF THE SOCIETY FOR EXPERIMENTAL BIOLOGY 28: 419 (1974).
- McClure, D. B., Cold Spring Harbor Conferences on Cell Proliferation 9: 345 (1982).
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Items 701–732 of 732
Schuldt, A. J. (2008) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm2572
Baker, N. M. (2019) · Carolina Digital Repository (University of North Carolina at Chapel Hill) · doi:10.17615/vf3z-jk38
Metabolic effects of oncogenic K-Ras in cancer cells
Bergner, R. (2018) · doi:10.25560/82596
Ras and Ras Signaling as a Therapeutic Target in Cancer
Sliker, B. H., & Campbell, P. M. (2021) · Elsevier eBooks · doi:10.1016/b978-0-12-820472-6.00006-2
Lopez, J. C. (2013) · Digital WPI
Ras Oncogenes and Cell Transformation
Marshall, C. J. (1987) · Springer eBooks · doi:10.1007/978-3-642-83118-8_23
Rappolee, D. A. (2003) · Humana Press eBooks · doi:10.1007/978-1-59259-335-4_3
Mechanisms of Endocytosis II Non-Clathrin
Parton, R. G., & Boucrot, E. (2022) · Elsevier eBooks · doi:10.1016/b978-0-12-821618-7.00026-2
Colombo, J. R., & Ukimura, O. (2006) · CRC Press eBooks · doi:10.3109/9781420021202-107
Swanson, J. A. (2022) · Elsevier eBooks · doi:10.1016/b978-0-12-821618-7.00058-4
The PMA Phorbol Ester Tumor Promoter Increases Canonical Wnt Signaling Via MacropinocytosisPreprint
Tejeda‐Muñoz, N., Azbazdar, Y., Monka, J., Binder, G., Dayrit, A., Ayala, R., O’Brien, N. A., & Robertis, E. M. D. (2023) · eLife · doi:10.7554/elife.89141.2
Tejeda‐Muñoz, N., Azbazdar, Y., Monka, J., Binder, G., Dayrit, A., Ayala, R., O’Brien, N. A., & Robertis, E. M. D. (2023) · eLife · doi:10.7554/elife.89141.2.sa3
Therapeutic Targeting of KRAS Oncogene in Pancreatic Ductal Adenocarcinoma (PDAC)
Song, J. J. (2024) · Journal of Student Research · doi:10.47611/jsrhs.v13i1.6375
Takeda, M., Theardy, M. S., Sorokin, A. V., Coker, O., Marie, P. K., Chen, S., Yang, Z., Nguyen, P. T.-M., Wei, Y., Yao, J., et al. (2024) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2024.08.06.606865
Yao, L., Zhang, W., Yang, X., Yi, M., & Jia, K. (2024) · eLife · doi:10.7554/elife.104772.1
Yao, L., Zhang, W., Yang, X., Yi, M., & Jia, K. (2025) · eLife · doi:10.7554/elife.104772.2
Yao, L., Zhang, W., Yang, X., Yi, M., & Jia, K. (2025) · eLife · doi:10.7554/elife.104772.3
Rosas, I. O., McDowell-Sanchez, A. K., Sanchez, S., Cala-García, J., Cohen, A. R. W., Echartea, M. E. R., Ochsner, S. A., Kraushaar, D. C., Celada, L. J., Sun, D., et al. (2025) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2025.07.09.663937
PP2A activation drives aberrant macropinocytosis and cell death in pancreatic ductal adenocarcinomaPreprint
Baral, G., Pfeffer, C. M., Filippelli, S. N., Doraivel, I., Heil, B. N., Gartenhaus, L. E., Hansen, K., Wu, J., Narla, G., Doud, E. H., et al. (2025) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2025.07.14.664742
Ras signalling at the actin cortex: Coordinating local and global changes to cell morphology
Tyler, J. J., & Matthews, H. K. (2025) · Current Opinion in Cell Biology · doi:10.1016/j.ceb.2025.102600
Lazareth, H. (2019) · theses.fr (ABES) · doi:10.70675/d4895365zd459z4e19za2bcza381f1de771a
Plant virus-derived nanoparticles for the imaging and treatment of cancer
Gamper, C. (2019) · theses.fr (ABES) · doi:10.70675/844eefddz7e34z4b59z978bzda1d6c32d297
Modulation of Oncogenic KRAS Signaling by Branched Actin-driven Cell Membrane ProtrusionsPreprint
Gihana, G. M., Bhatt, K., Chang, B.-J., Murali, V. S., Zhou, F., Noh, J., Ravishankar, R., Borges, H., Lin, J., Oceguera, J., et al. (2026) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.64898/2026.04.09.717047
Robb, R. N., Ackermann, S. E., Degan, S. E., Taylor, K. E., Yang, R. Y., Marler, A. Z., Calvert, V. S., Pierobon, M., Lyons, S. P., Barker, N. K., et al. (2026) · Cancer Research Communications · doi:10.1158/2767-9764.crc-25-0731 · Cited by 2
Functional Specialization of Clathrin‐Independent Endocytic Routes: Mechanisms and Cellular Roles
Benvenuto, A. F., Fardin, A., Balestra, M., Fiore, P. P. D., Tordonato, C., & Sigismund, S. (2026) · Traffic · doi:10.1111/tra.70049
BindingDB (2021) · UC San Diego · doi:10.7270/q2n87f26
Signal transduction therapy: a new paradigm
Kohn, E. C. (1995) · Springer eBooks · doi:10.1007/978-1-4757-0136-4_26
Metadata
- Authors
- Bar-Sagi, D., & Feramisco, J. R.
- Affiliations
- Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724
- Cold Spring Harbor Laboratory (US)
- Venue
- Science
- Published
- 1986-09-05
- Volume
- 233
- Issue
- 4768
- Pages
- 1061-1068
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W1969560454
- Cited by
- 734
- References
- 60
- Citations by year
- 2026: 6 · 2025: 15 · 2024: 17 · 2023: 23 · 2022: 17 · 2021: 21 · 2020: 17 · 2019: 25 · 2018: 23 · 2017: 19 · 2016: 14 · 2015: 25 · 2014: 14 · 2013: 11 · 2012: 5
- FWCI
- 14.12
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Science (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Cancer Institute (CA39811, CA07896)
- National Institute of General Medical Sciences (GM 28277)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Kinase Regulation and GTPase Signaling
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
- Keywords
- H-Ras, oncogenic Ras, ruffles, fluid-phase pinocytosis, quiescent fibroblasts, phospholipase A2, cell membrane dynamics, microinjection, tumor initiation
- MeSH terms
- Animals, Cell Cycle, Cell Membrane, Cells, Cultured, Culture Media, DNA, Humans, Microinjections, Oncogene Proteins, Viral, Phospholipases A, Phospholipids, Pinocytosis, Time Factors, GTP-Binding Proteins, Rats, Phospholipases A2
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