Two Pairs of Recombination Signals Are Sufficient to Cause Immunoglobulin V-(D)-J Joining
Abstract
The minimum sequence requirements for antigen receptor V-(D)-J joining were studied by constructing recombination-substrates containing synthetic recombination signals and introducing them into a recombination-competent pre-B cell line. Two sets of heptamer (CACTGTG) and nonamer (GGTTTTTGT) sequences were shown to be sufficient to cause the V-(D)-J joining, if the 12- and 23-base pair spacer rule is satisfied. A point mutation in the heptamer sequence, or a change in the combination of the two spacer lengths, drastically reduced the recombination.
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The structure of Transib TIRs corresponds closely to the structure of RSSs, which are composed of conserved heptamers 5′-CACAGTG separated by variable 22-bp spacers from ACAAAAACC-like nonamers.
“This corresponds closely to the structure of RSSs, which are composed of the conserved heptamers 5′- CACAGTG separated by a variable 22-bp spacers from ACAAAAACC-like nonamers [1,5–7].”
From RAG1 Core and V(D)J Recombination Signal Sequences Were Derived from Transib Transposons (2005) · cites this paper as [5]
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References 34 · Cited by 218
- Authors
- Akira, S., Okazaki, K., & Sakano, H.
- Affiliations
- University of California, Berkeley (US)
- ORCID
- Akira, Shizuo
- Venue
- Science
- Published
- 1987-11-20
- Volume
- 238
- Issue
- 4830
- Pages
- 1134-1138
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2106188260
- Cited by
- 218
- References
- 34
- Citations by year
- 2025: 3 · 2023: 1 · 2021: 3 · 2020: 2 · 2019: 2 · 2018: 4 · 2017: 4 · 2016: 5 · 2015: 5 · 2014: 1 · 2013: 4 · 2012: 4
- FWCI
- 4.30
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Science (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institute of Allergy and Infectious Diseases (AI18790)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA Repair Mechanisms
- Life Sciences › Immunology and Microbiology › Immunology › T-cell and B-cell Immunology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Keywords
- V(D)J recombination, immunoglobulin gene rearrangement, heptamer, spacer length, pre-B cells, antigen receptor gene rearrangement
- MeSH terms
- Animals, Base Sequence, Genes, Immunoglobulin, Genetic Vectors, Humans, Immunoglobulin Variable Region, Immunoglobulin kappa-Chains, Chromosome Inversion, Receptors, Antigen, T-Cell, Recombination, Genetic, Retroviridae, Sequence Homology, Nucleic Acid, Structure-Activity Relationship, Mice
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