Cytokine-Induced Expression of HIV-1 in a Chronically Infected Promonocyte Cell Line
Abstract
A model system for cytokine-induced up-regulation of human immunodeficiency virus type 1 (HIV-1) expression in chronically infected promonocyte clones was established. The parent promonocyte cell line U937 was chronically infected with HIV-1 and from this line a clone, U1, was derived. U1 showed minimal constitutive expression of HIV-1, but virus expression was markedly up-regulated by a phytohemagglutinin-induced supernatant containing multiple cytokines and by recombinant granulocyte/macrophage colony-stimulating factor alone. Recombinant interleukin-1 (IL-1), IL-2, interferon-γ, and tumor necrosis factor-α did not up-regulate virus expression. Concomitant with the cytokine-induced up-regulation of HIV-1, expression of membrane-bound IL-1β was selectively induced in U1 in the absence of induction of other surface membrane proteins. This cytokine up-regulation of IL-1β was not seen in the uninfected parent U937 cell line. These studies have implications for the understanding of the mechanism of progression from a latent or low-level HIV-1 infection to a productive infection with resulting immunosuppression. In addition, this model can be used to delineate the potential mechanisms whereby HIV-1 infection regulates cellular gene expression.
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U1 is a chronically HIV-1 infected promonocytic cell subclone that exhibits low-level constitutive viral gene expression and replication and is an HIV-1 latency model for infected perivascular macrophages and monocytes.
“The promonocytic U-937 cell subclone U1, an HIV-1 latency model for infected perivascular macrophages and monocytes, is chronically HIV-1 infected and exhibits low-level constitutive viral gene expression and replication”
From RNA-directed gene editing specifically eradicates latent and prevents new HIV-1 infection (2014) · cites this paper as [19]
Cites
An inducible transcription factor activates expression of human immunodeficiency virus in T cells
Nabel, G. J., & Baltimore, D. (1987) · Nature · doi:10.1038/326711a0 · Cited by 2,076 · see citing works
The Role of Mononuclear Phagocytes in HTLV-III/LAV Infection
Gartner, S., Markovits, P., Markovitz, D. M., Kaplan, M. H., Gallo, R. C., & Popovič, M. (1986) · Science · doi:10.1126/science.3014648 · Cited by 1,808 · see citing works
Detection of AIDS Virus in Macrophages in Brain Tissue from AIDS Patients with Encephalopathy
Koenig, S., Gendelman, H. E., Orenstein, J. M., et al. (1986) · Science · doi:10.1126/science.3016903 · Cited by 1,704 · see citing works
Dinarello, C. A. (1984) · Clinical Infectious Diseases · doi:10.1093/clinids/6.1.51 · Cited by 1,647 · see citing works
Self-citationThere is more than one interleukin 1
Oppenheim, J. J., Kovacs, E. J., Matsushima, K., & Durum, S. K. (1986) · Immunology Today · doi:10.1016/0167-5699(86)90124-6 · Cited by 1,203 · see citing works
Selective Tropism of Lymphadenopathy Associated Virus (LAV) for Helper-Inducer T Lymphocytes
Klatzmann, D., Barré‐Sinoussi, F., Nugeyre, M.-T., Danguet, C., VILMER, E., Griscelli, C., Brun‐Vézinet, F., Rouzioux, C., Gluckman, J. C., Chermann, J., et al. (1984) · Science · doi:10.1126/science.6328660 · Cited by 969 · see citing works
Dual Infection of the Central Nervous System by AIDS Viruses with Distinct Cellular Tropisms
Koyanagi, Y., Miles, S. A., Mitsuyasu, R. T., et al. (1987) · Science · doi:10.1126/science.3646751 · Cited by 834 · see citing works
Rosen, C. A., Sodroski, J., & Haseltine, W. A. (1985) · Cell · doi:10.1016/s0092-8674(85)80062-3 · Cited by 824 · see citing works
Infection of monocyte/macrophages by human T lymphotropic virus type III.
Ho, D. D., Rota, T. R., & Hirsch, M. (1986) · Journal of Clinical Investigation · doi:10.1172/jci112491 · Cited by 680 · see citing works
Feinberg, M. B., Jarrett, R. F., Aldovini, A., Gallo, R. C., & Wong‐Staal, F. (1986) · Cell · doi:10.1016/0092-8674(86)90062-0 · Cited by 667 · see citing works
Identification of a membrane-associated interleukin 1 in macrophages.
Kurt‐Jones, E. A., Beller, D. I., Mizel, S. B., & Unanue, E. R. (1985) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.82.4.1204 · Cited by 492 · see citing works
Folks, T. M., Powell, D., Lightfoote, M. M., Koenig, S., Fauci, A. S., Benn, S., Rabson, A. B., Daugherty, D. F., Gendelman, H. E., & HOGGAN, M. D. (1986) · The Journal of Experimental Medicine · doi:10.1084/jem.164.1.280 · Cited by 416 · see citing works
Self-citationTrans-activation of the human immunodeficiency virus long terminal repeat sequence by DNA viruses.
Gendelman, H. E., Phelps, W. C., Feigenbaum, L., Ostrove, J. M., Adachi, A., Howley, P. M., Khoury, G., Ginsberg, H. S., & Martin, M. A. (1986) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.83.24.9759 · Cited by 362 · see citing works
Nicholson, J. K. A., Cross, G. D., Callaway, C. S., et al. (1986) · The Journal of Immunology · doi:10.4049/jimmunol.137.1.323 · Cited by 340 · see citing works
Folks, T. M., Benn, S., Rabson, A. B., et al. (1985) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.82.13.4539 · Cited by 321 · see citing works
Self-citationGartner, S. (1986) · JAMA · doi:10.1001/jama.1986.03380170081023 · Cited by 319 · see citing works
Induction of HTLV-III/LAV from a Nonvirus-Producing T-Cell Line: Implications for Latency
Folks, T. M., Powell, D. M., Lightfoote, M. M., Benn, S., Martin, M. A., & Fauci, A. S. (1986) · Science · doi:10.1126/science.3003906 · Cited by 260 · see citing works
Self-citationReplicative and Cytopathic Potential of HTLV-III/LAV with sor Gene Deletions
Sodroski, J., Goh, W. C., Rosen, C. A., et al. (1986) · Science · doi:10.1126/science.3006244 · Cited by 214 · see citing works
Margolick, J. B., Volkman, D. J., Folks, T. M., et al. (1987) · The Journal of Immunology · doi:10.4049/jimmunol.138.6.1719 · Cited by 193 · see citing works
Self-citationClapham, P. R., Weiss, R. A., Dalgleish, A. G., Exley, M. A., Whitby, D., & Hogg, N. (1987) · Virology · doi:10.1016/0042-6822(87)90236-4 · Cited by 184 · see citing works
Induction of membrane-associated interleukin 1 by tumor necrosis factor in human fibroblasts.
Le, J., Weinstein, D., Gubler, U., & Vilček, J. (1987) · The Journal of Immunology · doi:10.4049/jimmunol.138.7.2137 · Cited by 169 · see citing works
Susceptibility of normal human lymphocytes to infection with HTLV-III/LAV.
Folks, T., Kelly, J. B., Benn, S., et al. (1986) · The Journal of Immunology · doi:10.4049/jimmunol.136.11.4049 · Cited by 164 · see citing works
Self-citationMelbye, M., BIGGAR, R. J., EBBESEN, P., et al. (1986) · Annals of Internal Medicine · doi:10.7326/0003-4819-104-4-496 · Cited by 163 · see citing works
Persistent Noncytopathic Infection of Normal Human T Lymphocytes with AIDS-Associated Retrovirus
Hoxie, J. A., Haggarty, B., Rackowski, J. L., et al. (1985) · Science · doi:10.1126/science.2994222 · Cited by 143 · see citing works
Hammer, S. M., Gillis, J. M., Groopman, J. E., & Rose, R. M. (1986) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.83.22.8734 · Cited by 130 · see citing works
Gerrard, T. L., Siegel, J. P., Dyer, D. R., et al. (1987) · The Journal of Immunology · doi:10.4049/jimmunol.138.8.2535 · Cited by 96 · see citing works
Harris, P. E., Ralph, P., Litcofsky, P. B., et al. (1985) · PubMed · Cited by 95 · see citing works
Interferon blocks interleukin 1-induced prostaglandin release from human peripheral monocytes.
Browning, J. L., & Ribolini, A. (1987) · The Journal of Immunology · doi:10.4049/jimmunol.138.9.2857 · Cited by 88 · see citing works
Petit, A. J., Terpstra, F. G., & Miedema, F. (1987) · Journal of Clinical Investigation · doi:10.1172/jci113032 · Cited by 85 · see citing works
T cell induction of membrane IL 1 on macrophages.
Weaver, C. T., & Unanue, E. R. (1986) · The Journal of Immunology · doi:10.4049/jimmunol.137.12.3868 · Cited by 77 · see citing works
Amento, E. P., Kurnick, J. T., & Krane, S. M. (1985) · The Journal of Immunology · doi:10.4049/jimmunol.134.1.350 · Cited by 44 · see citing works
Hagiwara, H., Huang, H. J. S., Arai, N., et al. (1987) · The Journal of Immunology · doi:10.4049/jimmunol.138.8.2514 · Cited by 36 · see citing works
IL 1 induction by murine T cell clones: detection of an IL 1-inducing lymphokine.
Takács, L., Berzofsky, J. A., York-Jolley, J., et al. (1987) · The Journal of Immunology · doi:10.4049/jimmunol.138.7.2124 · Cited by 23 · see citing works
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Hu, W., Kaminski, R., Yang, F., Zhang, Y., Cosentino, L., Li, F., Luo, B., Alvarez-Carbonell, D., Garcia-Mesa, Y., Karn, J., et al. (2014) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1405186111 · Cited by 527 · see citing works
Poli, G., Kinter, A., Justement, J. S., Kehrl, J. H., Bressler, P., Stanley, S., & Fauci, A. S. (1990) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.87.2.782 · Cited by 457 · see citing works
Self-citationDoyon, G., Zerbato, J., Mellors, J. W., & Sluis-Cremer, N. (2013) · AIDS · doi:10.1097/qad.0b013e3283570620 · Cited by 107 · see citing works
Fan, S., Hsia, K., & Edgington, T. (1994) · Blood · doi:10.1182/blood.v84.5.1567.1567 · Cited by 38 · see citing works
Borghi, M. O., Panzeri, P., Shattock, R., Sozzani, S., Dobrina, A., & Meroni, P. L. (2001) · Clinical and Experimental Immunology · doi:10.1046/j.1365-2249.2000.01186.x · Cited by 21 · see citing works
- Authors
- Folks, T. M., Justement, J., Kinter, A., Dinarello, C. A., & Fauci, A. S.
- Affiliations
- National Institutes of Health (US)
- Tufts University (US)
- Venue
- Science
- Published
- 1987-11-06
- Volume
- 238
- Issue
- 4828
- Pages
- 800-802
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2036890038
- Cited by
- 905
- References
- 33
- Citations by year
- 2026: 4 · 2025: 8 · 2024: 16 · 2023: 7 · 2022: 11 · 2021: 9 · 2020: 15 · 2019: 17 · 2018: 10 · 2017: 12 · 2016: 14 · 2015: 17 · 2014: 19 · 2013: 18 · 2012: 20
- FWCI
- 10.47
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Science (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Immunology and Microbiology › Virology › HIV Research and Treatment
- Life Sciences › Immunology and Microbiology › Immunology › T-cell and Retrovirus Studies
- Health Sciences › Medicine › Infectious Diseases › HIV/AIDS drug development and treatment
- Keywords
- HIV-1, chronic HIV-1 infection, GM-CSF, phytohemagglutinin, IL-1β, interleukin-2, interferon-gamma, TNF-α, viral latency, HIV-1 gene expression regulation, immunosuppression
- MeSH terms
- Biological Products, Cell Line, Clone Cells, HIV, Monocytes, Recombinant Proteins, Cytokines
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