The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Generation of cDNA Probes Directed by Amino Acid Sequence: Cloning of Urate Oxidase
Abstract
Urate oxidase (E.C. 1.7.3.3) catalyzes the oxidation of uric acid to allantoin in most mammals except humans and certain primates. The amino-terminal amino acid sequence for porcine urate oxidase was determined and used in a novel procedure for generating complementary DNA (cDNA) probes to this amino acid sequence. The procedure is based on the polymerase chain reaction and utilizes mixed oligonucleotide primers complementary to the reverse translation products of an amino acid sequence. This rapid and simple cDNA cloning procedure is generally applicable and requires only a partial amino acid sequence. A cDNA probe developed by this procedure was used to isolate a full-length porcine urate oxidase cDNA and to demonstrate the presence of homologous genomic sequences in humans.
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Cites
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Cited by
Frohman, M. A., Dush, M. K., & Martin, G. R. (1988) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.85.23.8998 · Cited by 4,577
Chen, H., Charlat, O., Tartaglia, L. A., Woolf, E. A., Weng, X., Ellis, S. J., Lakey, N. D., Culpepper, J., More, K. J., Breitbart, R. E., et al. (1996) · Cell · doi:10.1016/s0092-8674(00)81294-5 · Cited by 2,214
Recent Advances in the Polymerase Chain Reaction
Erlich, H. A., Gelfand, D., & Sninsky, J. J. (1991) · Science · doi:10.1126/science.2047872 · Cited by 1,077
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Kieran, M., Blank, V., Logeat, F., Vandekerckhove, J., Lottspeich, F., Le Bail, O., Urban, M. B., Kourilsky, P., Baeuerle, P. A., & Israël, A. (1990) · Cell · doi:10.1016/0092-8674(90)90275-j · Cited by 874
Metadata
- Authors
- Lee, C. C., Wu, X., Gibbs, R. A., Cook, R. G., Muzny, D. M., & Caskey, C. T.
- Venue
- Science
- Published
- 1988-03-11
- Volume
- 239
- Issue
- 4845
- Pages
- 1288-1291
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W1970627065
- Cited by
- 319
- References
- 1
- FWCI
- 49.93
- Type
- journal article
- Language
- en
- Open access
- closed
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- Lee, C. C., Wu, X., Gibbs, R. A., Cook, R. G., Muzny, D. M., & Caskey, C. T. (1988). Generation of cDNA Probes Directed by Amino Acid Sequence: Cloning of Urate Oxidase. Science. https://doi.org/10.1126/science.3344434
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